{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T18:56:37Z","timestamp":1792004197602,"version":"4.4.0"},"reference-count":38,"publisher":"SAGE Publications","issue":"8","license":[{"start":{"date-parts":[[2019,10,1]],"date-time":"2019-10-01T00:00:00Z","timestamp":1569888000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Microbial Drug Resistance"],"published-print":{"date-parts":[[2019,10,1]]},"abstract":"<jats:sec>\n                    <jats:title>Objectives:<\/jats:title>\n                    <jats:p>\n                      The primary aim of this study was to investigate the presence of antibiotic resistance genes (ARGs) and plasmid replicon types for 75 multidrug-resistant (MDR)\n                      <jats:italic toggle=\"yes\">Salmonella<\/jats:italic>\n                      Enteritidis isolates from children with gastroenteritis. We also evaluated the association among biofilm formation,\n                      <jats:italic toggle=\"yes\">in vitro<\/jats:italic>\n                      invasion capacity, and antibiotic resistance phenotypes.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Materials and Methods:<\/jats:title>\n                    <jats:p>\n                      Twenty-two ARGs and 18 different plasmid incompatibility types were investigated using polymerase chain reaction (PCR) and DNA sequencing.\n                      <jats:italic toggle=\"yes\">In vitro<\/jats:italic>\n                      invasion capacity of\n                      <jats:italic toggle=\"yes\">S.<\/jats:italic>\n                      Enteritidis isolates possessing different antibiotic resistance patterns was assessed using the Caco2 human intestinal epithelial cell line and biofilm formation was performed in a 96-well polystyrene well format using crystal violet detection.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results:<\/jats:title>\n                    <jats:p>\n                      The presence of plasmid-mediated quinolone resistance genes and \u03b2-lactamase genes was established using PCR amplification. All the tested\n                      <jats:italic toggle=\"yes\">S.<\/jats:italic>\n                      Enteritidis isolates that were fluoroquinolone resistant possessed\n                      <jats:italic toggle=\"yes\">gyrA<\/jats:italic>\n                      mutations and 50% also possessed mutations in\n                      <jats:italic toggle=\"yes\">parC<\/jats:italic>\n                      . MDR\n                      <jats:italic toggle=\"yes\">S.<\/jats:italic>\n                      Enteritidis isolates containing three (29\/75) or four (21\/75) plasmid replicon types were predominant and 71\/75 carried both FIIs and FIC replicon-type plasmids. MDR isolates were strong or moderate biofilm producers and a significant positive association (\n                      <jats:italic toggle=\"yes\">p<\/jats:italic>\n                      \u2009&lt;\u20090.05) between antibiotic resistance and biomass of biofilms was observed in the strains assayed. A ceftiofur-resistant strain was significantly more invasive (\n                      <jats:italic toggle=\"yes\">p<\/jats:italic>\n                      \u2009&lt;\u20090.01) than the other isolates.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions:<\/jats:title>\n                    <jats:p>\n                      We observed a high incidence of ARGs and diversity of plasmids in\n                      <jats:italic toggle=\"yes\">S.<\/jats:italic>\n                      Enteritidis isolates from children. Biofilm formation and invasion capacity highlight a significant hazard to public health.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1089\/mdr.2018.0421","type":"journal-article","created":{"date-parts":[[2019,5,23]],"date-time":"2019-05-23T11:45:57Z","timestamp":1558611957000},"page":"1191-1198","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":15,"title":["Characterization of Antibiotic Resistance Genes, Plasmids, Biofilm Formation, and\n                    <i>In Vitro<\/i>\n                    Invasion Capacity of\n                    <i>Salmonella<\/i>\n                    Enteritidis Isolates from Children with Gastroenteritis"],"prefix":"10.1177","volume":"25","author":[{"given":"Xiujuan","family":"Zhou","sequence":"first","affiliation":[{"name":"MOST-USDA Joint Research Center for Food Safety, School of Agriculture and Biology, State Key Laboratory of Microbial Metabolism, Shanghai Jiao Tong University, Shanghai, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meiya","family":"Li","sequence":"additional","affiliation":[{"name":"Academy of Chinese Medical Sciences, Zhejiang Chinese Medical University, Hangzhou, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li","family":"Xu","sequence":"additional","affiliation":[{"name":"MOST-USDA Joint Research Center for Food Safety, School of Agriculture and Biology, State Key Laboratory of Microbial Metabolism, Shanghai Jiao Tong University, Shanghai, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chunlei","family":"Shi","sequence":"additional","affiliation":[{"name":"MOST-USDA Joint Research Center for Food Safety, School of Agriculture and Biology, State Key Laboratory of Microbial Metabolism, Shanghai Jiao Tong University, Shanghai, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xianming","family":"Shi","sequence":"additional","affiliation":[{"name":"MOST-USDA Joint Research Center for Food Safety, School of Agriculture and Biology, State Key Laboratory of Microbial Metabolism, Shanghai Jiao Tong University, Shanghai, China."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2019,10,11]]},"reference":[{"key":"e_1_3_4_2_2","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/civ729"},{"key":"e_1_3_4_3_2","article-title":"Changes in epidemiology and antimicrobial susceptibility of nontyphoid Salmonella in children in southern Taiwan, 1997\u20132016","author":"Lo H.Y.","year":"2018","unstructured":"Lo H.Y., , Lai F.P, , and Yang Y.J. 2018. 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