{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T19:29:09Z","timestamp":1777922949227,"version":"3.51.4"},"reference-count":19,"publisher":"SAGE Publications","issue":"8","license":[{"start":{"date-parts":[[2019,10,1]],"date-time":"2019-10-01T00:00:00Z","timestamp":1569888000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Microbial Drug Resistance"],"published-print":{"date-parts":[[2019,10,1]]},"abstract":"<jats:p>\n                    The dissemination of antimicrobial resistance genes and the bacterium that harbor them have increasingly become a public concern, especially in low- and middle-income countries. The present study used whole-genome sequencing to analyze 10 KPC-2-producing\n                    <jats:italic toggle=\"yes\">Klebsiella pneumoniae<\/jats:italic>\n                    isolates obtained from clinical specimens originated from Brazilian hospitals. The study documents a relevant \u201csnapshot\u201d of the presence of class 1 integrons in 90% of the strains presenting different gene cassettes (\n                    <jats:italic toggle=\"yes\">dfrA30, dfrA15, dfrA12, dfrA14, aadA1, aadA2,<\/jats:italic>\n                    and\n                    <jats:italic toggle=\"yes\">aac(6\u2032)Iq<\/jats:italic>\n                    ), associated or not with transposons. Two strains presented nonclassical integron (lacking the normal 3\u2032conserved segment). In general, most strains showed a complex resistome, characterizing them as highly resistant. Integrons, a genetically stable and efficient system, confer to bacteria as highly adaptive and low cost evolution potential to bacteria, even more serious when associated with high-risk clones, indicating an urgent need for control and prevention strategies to avoid the spread of resistance determinants in Brazil. Despite this, although the class 1 integron identified in the KPC-2-producing\n                    <jats:italic toggle=\"yes\">K. pneumoniae<\/jats:italic>\n                    clones is important, our findings suggest that other elements probably have a greater impact on the spread of antimicrobial resistance, since many of these important genes were not related to this cassette.\n                  <\/jats:p>","DOI":"10.1089\/mdr.2018.0437","type":"journal-article","created":{"date-parts":[[2019,5,10]],"date-time":"2019-05-10T10:11:43Z","timestamp":1557483103000},"page":"1127-1131","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":8,"title":["Molecular Detection of Class 1 Integron-Associated Gene Cassettes in KPC-2-Producing\n                    <i>Klebsiella pneumoniae<\/i>\n                    Clones by Whole-Genome Sequencing"],"prefix":"10.1177","volume":"25","author":[{"given":"Bruna","family":"Fuga","sequence":"first","affiliation":[{"name":"Laborat\u00f3rio de Microbiologia Molecular, Instituto de Ci\u00eancias Biom\u00e9dicas, Universidade Federal de Uberl\u00e2ndia, Uberl\u00e2ndia, Brazil."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sabrina","family":"Royer","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Microbiologia Molecular, Instituto de Ci\u00eancias Biom\u00e9dicas, Universidade Federal de Uberl\u00e2ndia, Uberl\u00e2ndia, Brazil."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paola Amaral de","family":"Campos","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Microbiologia Molecular, Instituto de Ci\u00eancias Biom\u00e9dicas, Universidade Federal de Uberl\u00e2ndia, Uberl\u00e2ndia, Brazil."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Melina Lorraine","family":"Ferreira","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Microbiologia Molecular, Instituto de Ci\u00eancias Biom\u00e9dicas, Universidade Federal de Uberl\u00e2ndia, Uberl\u00e2ndia, Brazil."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Iara","family":"Rossi","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Microbiologia Molecular, Instituto de Ci\u00eancias Biom\u00e9dicas, Universidade Federal de Uberl\u00e2ndia, Uberl\u00e2ndia, Brazil."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luiz Gustavo","family":"Machado","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Microbiologia Molecular, Instituto de Ci\u00eancias Biom\u00e9dicas, Universidade Federal de Uberl\u00e2ndia, Uberl\u00e2ndia, Brazil."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Louise Teixeira","family":"Cerdeira","sequence":"additional","affiliation":[{"name":"Faculdade de Ci\u00eancias Farmac\u00eauticas, Universidade de S\u00e3o Paulo, S\u00e3o Paulo, Brazil."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Deivid William da","family":"Fonseca Batist\u00e3o","sequence":"additional","affiliation":[{"name":"Faculdade de Medicina, Universidade Federal de Uberl\u00e2ndia, Uberl\u00e2ndia, Brazil."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cristiane Silveira de","family":"Brito","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Microbiologia Molecular, Instituto de Ci\u00eancias Biom\u00e9dicas, Universidade Federal de Uberl\u00e2ndia, Uberl\u00e2ndia, Brazil."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nilton","family":"Lincopan","sequence":"additional","affiliation":[{"name":"Faculdade de Ci\u00eancias Farmac\u00eauticas, Universidade de S\u00e3o Paulo, S\u00e3o Paulo, Brazil."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paulo Pinto","family":"Gontijo-Filho","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Microbiologia Molecular, Instituto de Ci\u00eancias Biom\u00e9dicas, Universidade Federal de Uberl\u00e2ndia, Uberl\u00e2ndia, Brazil."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rosineide Marques","family":"Ribas","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Microbiologia Molecular, Instituto de Ci\u00eancias Biom\u00e9dicas, Universidade Federal de Uberl\u00e2ndia, Uberl\u00e2ndia, Brazil."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2019,10,10]]},"reference":[{"key":"e_1_3_4_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/MMBR.00056-13"},{"key":"e_1_3_4_3_2","doi-asserted-by":"publisher","DOI":"10.1186\/s40168-018-0516-2"},{"key":"e_1_3_4_4_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0179169"},{"key":"e_1_3_4_5_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2012.00119"},{"key":"e_1_3_4_6_2","first-page":"MDNA3","article-title":"The integron: Adaptation on demand","volume":"3","author":"Escudero J.A.","year":"2014","unstructured":"Escudero J.A., , Loot C., , Nivina A., , and Mazel D. 2014. The integron: Adaptation on demand. Microbiol. Spectrum. 3:MDNA3-0019.","journal-title":"Microbiol. Spectrum"},{"key":"e_1_3_4_7_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0075805"},{"key":"e_1_3_4_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bjm.2016.10.002"},{"key":"e_1_3_4_9_2","doi-asserted-by":"publisher","DOI":"10.1093\/femsre\/fux013"},{"key":"e_1_3_4_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/S2214-109X(18)30278-X"},{"key":"e_1_3_4_11_2","unstructured":"CLSI. 2018. Performance Standards for Antimicrobial Susceptibility Testing; Document M100-S28. Clinical and Laboratory Standards Institute Wayne PA."},{"key":"e_1_3_4_12_2","unstructured":"Galetti R. 2010. Estudo de Pseudomonas aeruginosa produtoras de metalo-beta-lactamases e de genes envolvidos na resist\u00eancia aos carbapen\u00eamicos. Dissertation Universidade de S\u00e3o Paulo."},{"key":"e_1_3_4_13_2","doi-asserted-by":"publisher","DOI":"10.1099\/jmm.0.000711"},{"key":"e_1_3_4_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijantimicag.2018.08.011"},{"key":"e_1_3_4_15_2","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.000099"},{"key":"e_1_3_4_16_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01019-15"},{"key":"e_1_3_4_17_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dks214"},{"key":"e_1_3_4_18_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dks396"},{"key":"e_1_3_4_19_2","doi-asserted-by":"publisher","DOI":"10.1099\/jmm.0.053645-0"},{"key":"e_1_3_4_20_2","first-page":"1","article-title":"Mechanisms of antibiotic resistance","volume":"4","author":"Munita J.M.","year":"2016","unstructured":"Munita J.M., , and Arias C.A. 2016. Mechanisms of antibiotic resistance. 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