{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T19:29:34Z","timestamp":1777922974842,"version":"3.51.4"},"reference-count":40,"publisher":"SAGE Publications","issue":"7","license":[{"start":{"date-parts":[[2020,7,1]],"date-time":"2020-07-01T00:00:00Z","timestamp":1593561600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Microbial Drug Resistance"],"published-print":{"date-parts":[[2020,7,1]]},"abstract":"\n The dissemination of extended-spectrum \u03b2-lactamases encoding genes in\n Escherichia coli<\/jats:italic>\n , especially in the uropathogenic O25b-ST131\n E. coli<\/jats:italic>\n clone, constitutes a real concern. We aimed to identify the molecular mechanisms of resistance to cephalosporins among\n E. coli<\/jats:italic>\n clinical isolates and to estimate the prevalence of the uropathogenic O25b-ST131 clone in our study. Forty-two cephalosporin-resistant\n E. coli<\/jats:italic>\n implicated in urinary tract infections were collected from the Regional Hospital of a southeastern Tunisian Island from April 2015 to August 2016. Molecular screening of \u03b2-lactamases encoding genes by PCR and sequencing showed that the majority of our isolates harbored\n bla<\/jats:italic>\n CTX-M<\/jats:sub>\n gene (\n bla<\/jats:italic>\n CTX-M-15<\/jats:sub>\n [\n n<\/jats:italic>\n \u2009=\u200936],\n bla<\/jats:italic>\n CTX-M-14<\/jats:sub>\n [\n n<\/jats:italic>\n \u2009=\u20092]). Nevertheless, the\n bla<\/jats:italic>\n SHV<\/jats:sub>\n ,\n bla<\/jats:italic>\n TEM<\/jats:sub>\n , and\n bla<\/jats:italic>\n OXA-1<\/jats:sub>\n genes were not detected. Various class C \u03b2-lactamases encoding genes were observed in association or not with\n bla<\/jats:italic>\n CTX-M<\/jats:sub>\n genes and were as follows:\n bla<\/jats:italic>\n ampC<\/jats:sub>\n (\n n<\/jats:italic>\n \u2009=\u200914),\n bla<\/jats:italic>\n CMY-42<\/jats:sub>\n (\n n<\/jats:italic>\n \u2009=\u20097),\n bla<\/jats:italic>\n CMY-2<\/jats:sub>\n (\n n<\/jats:italic>\n \u2009=\u20091), and\n bla<\/jats:italic>\n DHA-4<\/jats:sub>\n (\n n<\/jats:italic>\n \u2009=\u20091). The research of O25b-ST131 clone was carried out by duplex PCR (\n pab<\/jats:italic>\n B and\n trp<\/jats:italic>\n A genes) and revealed that most of our isolates (\n n<\/jats:italic>\n \u2009=\u200930) belonged to this clone. We also noted that the majority of our isolates belonged to the B2 phylogenetic group (\n n<\/jats:italic>\n \u2009=\u200932), five isolates to the B1 phylogenetic group, three isolates to the D phylogenetic group, and only two isolates belonged to the A phylogenetic group. Our study provides new epidemiological information about\n E. coli<\/jats:italic>\n clinical isolates in this area. Indeed, this is the first report of CTX-M-14 producing O25b-ST131\n E. coli<\/jats:italic>\n in our country and the first report of DHA-4 and CMY-42 producing\n E. coli<\/jats:italic>\n in Tunisia.\n <\/jats:p>","DOI":"10.1089\/mdr.2019.0076","type":"journal-article","created":{"date-parts":[[2020,1,9]],"date-time":"2020-01-09T11:13:41Z","timestamp":1578568421000},"page":"741-746","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":11,"title":["Characterization of O25b-ST131\n Escherichia coli<\/i>\n Clone Producing CTX-M-15, DHA-4, and CMY-42 in Urinary Tract Infections in a Tunisian Island"],"prefix":"10.1177","volume":"26","author":[{"given":"Olfa","family":"Dziri","sequence":"first","affiliation":[{"name":"Laboratoire des Microorganismes et Biomol\u00e9cules Actives LR03ES03, Facult\u00e9 des Sciences de Tunis, Universit\u00e9 de Tunis El Manar, El-Manar II, Tunisia."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Raoudha","family":"Dziri","sequence":"additional","affiliation":[{"name":"Laboratoire des Microorganismes et Biomol\u00e9cules Actives LR03ES03, Facult\u00e9 des Sciences de Tunis, Universit\u00e9 de Tunis El Manar, El-Manar II, Tunisia."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Abderrahmen","family":"Maraoub","sequence":"additional","affiliation":[{"name":"H\u00f4pital R\u00e9gional Sadok Mkaddem de Djerba, Avenue Habib Bourguiba Houmet Souk Djerba, Tunis, Tunisia."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chedly","family":"Chouchani","sequence":"additional","affiliation":[{"name":"Laboratoire des Microorganismes et Biomol\u00e9cules Actives LR03ES03, Facult\u00e9 des Sciences de Tunis, Universit\u00e9 de Tunis El Manar, El-Manar II, Tunisia."},{"name":"Laboratoire de Recherche Sciences et Technologies de l'Environnement LR16ES09, Institut Sup\u00e9rieur des Sciences et Technologies de l' Environnement de Borj-Cedria, Universit\u00e9 de Carthage, Technop\u00f4le de Borj-Cedria, Hammam-Lif, Tunisia."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2020,7,7]]},"reference":[{"key":"e_1_3_4_2_2","doi-asserted-by":"publisher","DOI":"10.1542\/pir.36.4.167"},{"key":"e_1_3_4_3_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrurol.2010.190"},{"key":"e_1_3_4_4_2","first-page":"286","volume":"51","author":"Johnson J.","year":"2010","unstructured":"Johnson J., , Johnston B., , Clabots C., , Kuskowski M., , and Castanheira M.. 2010. Escherichia coli sequence type ST131 as the major cause of serious multidrug-resistant E. coli infections in the United States. Clin. Infect. Dis. 51:286\u2013294.","journal-title":"Escherichia coli sequence type ST131 as the major cause of serious multidrug-resistant E. coli infections in the United States. Clin. Infect. 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Plasmid-mediated extended-spectrum beta-lactamase (CTX-M-3 like) from India and gene association with insertion sequence ISEcp1. FEMS Microbiol. Lett. 201:237\u2013241.","journal-title":"FEMS Microbiol. Lett"},{"key":"e_1_3_4_14_2","doi-asserted-by":"publisher","DOI":"10.1089\/mdr.2005.11.58"},{"key":"e_1_3_4_15_2","first-page":"85","article-title":"Extended spectrum \u03b2-lactamases producing Klebsiella pneumoniae from the neonatal intensive care unit at the university teaching hospital in Lusaka, Zambia","volume":"4","author":"Mumbula E.","year":"2015","unstructured":"Mumbula E., , Kwenda G., , Samutela M., , Kalonda A., , Mwansa J., , Mwenya D., , Koryolova L., , Kaile T., , Marimo C., , and Lukwesa-Musyani C.. 2015. Extended spectrum \u03b2-lactamases producing Klebsiella pneumoniae from the neonatal intensive care unit at the university teaching hospital in Lusaka, Zambia. J. Med. Sci. Technol. 4:85\u201391.","journal-title":"J. Med. Sci. 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