{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T19:29:34Z","timestamp":1777922974886,"version":"3.51.4"},"reference-count":29,"publisher":"SAGE Publications","issue":"3","license":[{"start":{"date-parts":[[2020,3,1]],"date-time":"2020-03-01T00:00:00Z","timestamp":1583020800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Microbial Drug Resistance"],"published-print":{"date-parts":[[2020,3]]},"abstract":"\n The emergence of\n bla<\/jats:italic>\n NDM<\/jats:sub>\n particularly in Gram-negative bacteria is a burden on the health care system in developing countries. Hence, this study was initiated to screen New Delhi Metallo-\u03b2-lactamase (NDM)-producing Gram-negative bacterial strains from neonatal intensive care unit (NICU) of an Indian Hospital. A total of 18\n bla<\/jats:italic>\n NDM<\/jats:sub>\n -producing isolates were detected in the present study. Out of 18\n bla<\/jats:italic>\n NDM<\/jats:sub>\n variant isolates, 6 were\n Klebsiella pneumoniae<\/jats:italic>\n , 4\n Escherichia coli<\/jats:italic>\n , 2\n Enterobacter aerogenes<\/jats:italic>\n , 1\n Acinetobacter lwoffii<\/jats:italic>\n , 1\n Enterobacter cloacae<\/jats:italic>\n , 3\n Acinobacter baumannii,<\/jats:italic>\n and 1\n Cedecea davisae<\/jats:italic>\n from NICU, showing resistance against all antibiotics, except colistin and polymixin. The transferability of resistance determinants was tested by conjugation. Transfer of\n bla<\/jats:italic>\n NDM<\/jats:sub>\n -producing strains was successful in all 18 strains. In the case of transconjugants, the minimum inhibitory concentration values were found to decrease. The\n bla<\/jats:italic>\n NDM<\/jats:sub>\n -producing isolates contained detectable plasmids of size 66, 38, and 6\u2009kb. Plasmi\/d-based replicon typing revealed the incompatibility types Inc (A\/C, FIIA, FIC, K, F, W, FIA, P, X, FIB, B\/O) in\n bla<\/jats:italic>\n NDM<\/jats:sub>\n -carrying isolates. This study revealed the outbreak of multiple variants of\n bla<\/jats:italic>\n NDM<\/jats:sub>\n (13 NDM-1, 4 NDM-5, and 1 NDM-7). Moreover, other resistance markers, viz.\n bla<\/jats:italic>\n OXA-1<\/jats:sub>\n ,\n \n bla\n C<\/jats:sub>\n <\/jats:italic>\n MY-1<\/jats:sub>\n ,\n bla<\/jats:italic>\n VIM-1<\/jats:sub>\n , and\n bla<\/jats:italic>\n SHV-1<\/jats:sub>\n coassociated with\n bla<\/jats:italic>\n NDM<\/jats:sub>\n were also found. In this study, we reported NDM\u2013producing\n C. davisae<\/jats:italic>\n as a first report to the best of our knowledge. This study is an attempt to reveal the dissemination of\n bla<\/jats:italic>\n NDM<\/jats:sub>\n isolated from neonates in NICU and their efficient transferability among Gram-negative bacilli through horizontal gene transfer.\n <\/jats:p>","DOI":"10.1089\/mdr.2019.0092","type":"journal-article","created":{"date-parts":[[2019,8,9]],"date-time":"2019-08-09T10:43:20Z","timestamp":1565347400000},"page":"284-289","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":32,"title":["Outbreak of Efficiently Transferred Carbapenem-Resistant\n bla<\/i>\n NDM<\/sub>\n -Producing Gram-Negative Bacilli Isolated from Neonatal Intensive Care Unit of an Indian Hospital"],"prefix":"10.1177","volume":"26","author":[{"given":"Shamsi","family":"Khalid","sequence":"first","affiliation":[{"name":"Medical Microbiology and Molecular Biology Laboratory, Interdisciplinary Biotechnology Unit, Aligarh Muslim University, Aligarh, India."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nayeem","family":"Ahmad","sequence":"additional","affiliation":[{"name":"Medical Microbiology and Molecular Biology Laboratory, Interdisciplinary Biotechnology Unit, Aligarh Muslim University, Aligarh, India."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Seyed M.","family":"Ali","sequence":"additional","affiliation":[{"name":"Peripatetics Department, JN Medical College and Hospital, Aligarh Muslim University, Aligarh, India."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Asad U.","family":"Khan","sequence":"additional","affiliation":[{"name":"Medical Microbiology and Molecular Biology Laboratory, Interdisciplinary Biotechnology Unit, Aligarh Muslim University, Aligarh, India."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2020,3,5]]},"reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"crossref","first-page":"182","DOI":"10.1136\/archdischild-2011-300097","article-title":"Multi-drug resistant Gram negative bacilli causing early neonatal sepsis in India","volume":"97","author":"Viswanathan R.","year":"2012","unstructured":"Viswanathan R., , Singh A.K, , Basu S., , Chatterjee S., , Sardar S., , and Isaacs D.. 2012. 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