{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T19:29:57Z","timestamp":1777922997696,"version":"3.51.4"},"reference-count":36,"publisher":"SAGE Publications","issue":"5","license":[{"start":{"date-parts":[[2020,5,1]],"date-time":"2020-05-01T00:00:00Z","timestamp":1588291200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Microbial Drug Resistance"],"published-print":{"date-parts":[[2020,5]]},"abstract":"<jats:p>\n                    Surface water is suspected of playing a role in the development and spread of antimicrobial-resistant (AR) bacteria, including human pathogens. In our previous study, 496\n                    <jats:italic toggle=\"yes\">Escherichia coli<\/jats:italic>\n                    isolates were recovered from water samples collected over a 2-year period from the Upper Oconee watershed, Athens, GA, United States, of which 34 (6.9%) were AR isolates. Of these, six isolates were selected based on their multidrug resistance (MDR) phenotypes, the presence of mobile genetic elements, and their pathogenic potential and were subjected to whole-genome sequence (WGS) analysis to enhance our understanding of environmental MDR\n                    <jats:italic toggle=\"yes\">E. coli<\/jats:italic>\n                    isolates. This study is the first report on genomic characterization of MDR\n                    <jats:italic toggle=\"yes\">E. coli<\/jats:italic>\n                    from environmental water in the United States through a WGS approach. The sequences of the six MDR\n                    <jats:italic toggle=\"yes\">E. coli<\/jats:italic>\n                    isolates were analyzed and the locations of their AR genes were identified. One of the\n                    <jats:italic toggle=\"yes\">E. coli<\/jats:italic>\n                    isolates was an ST131 epidemic strain, which also produced an extended-spectrum \u03b2-lactamase encoded by the\n                    <jats:italic toggle=\"yes\">bla<\/jats:italic>\n                    <jats:sub>CTX-M-15<\/jats:sub>\n                    gene, carried on a plasmid that is a member of a very rarely reported family of phage-like plasmids. This is the first time an in-depth sequence analysis has been done on a\n                    <jats:italic toggle=\"yes\">bla<\/jats:italic>\n                    <jats:sub>CTX-M-15<\/jats:sub>\n                    - containing phage-like plasmid, the presence of which suggests a new emerging mechanism of AR gene transmission.\n                  <\/jats:p>","DOI":"10.1089\/mdr.2019.0306","type":"journal-article","created":{"date-parts":[[2019,11,14]],"date-time":"2019-11-14T12:02:10Z","timestamp":1573732930000},"page":"447-455","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":6,"title":["Genomic Analysis of Multidrug-Resistant\n                    <i>Escherichia coli<\/i>\n                    from Surface Water in Northeast Georgia, United States: Presence of an ST131 Epidemic Strain Containing\n                    <i>bla<\/i>\n                    <sub>CTX-M-15<\/sub>\n                    on a Phage-Like Plasmid"],"prefix":"10.1177","volume":"26","author":[{"given":"Sohyun","family":"Cho","sequence":"first","affiliation":[{"name":"Department of Microbiology, University of Georgia, Athens, Georgia, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sushim K.","family":"Gupta","sequence":"additional","affiliation":[{"name":"Bacterial Epidemiology and Antimicrobial Resistance Research Unit, Agricultural Research Service, U.S. Department of Agriculture, Athens, Georgia, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elizabeth A.","family":"McMillan","sequence":"additional","affiliation":[{"name":"Department of Microbiology, University of Georgia, Athens, Georgia, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Poonam","family":"Sharma","sequence":"additional","affiliation":[{"name":"Bacterial Epidemiology and Antimicrobial Resistance Research Unit, Agricultural Research Service, U.S. Department of Agriculture, Athens, Georgia, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hazem","family":"Ramadan","sequence":"additional","affiliation":[{"name":"Hygiene and Zoonoses Department, Faculty of Veterinary Medicine, Mansoura University, Mansoura, Egypt."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thomas","family":"Jov\u00e9","sequence":"additional","affiliation":[{"name":"Univ. Limoges, INSERM, CHU Limoges, RESINFIT, U1092, Limoges, France."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Charlene R.","family":"Jackson","sequence":"additional","affiliation":[{"name":"Bacterial Epidemiology and Antimicrobial Resistance Research Unit, Agricultural Research Service, U.S. Department of Agriculture, Athens, Georgia, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jonathan G.","family":"Frye","sequence":"additional","affiliation":[{"name":"Bacterial Epidemiology and Antimicrobial Resistance Research Unit, Agricultural Research Service, U.S. Department of Agriculture, Athens, Georgia, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2020,5,7]]},"reference":[{"key":"e_1_3_6_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.11.1.142"},{"key":"e_1_3_6_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijmm.2005.07.002"},{"key":"e_1_3_6_4_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2013.00258"},{"key":"e_1_3_6_5_2","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/cit355"},{"key":"e_1_3_6_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00002-11"},{"key":"e_1_3_6_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mib.2006.08.011"},{"key":"e_1_3_6_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(08)70041-0"},{"key":"e_1_3_6_9_2","doi-asserted-by":"crossref","first-page":"161","DOI":"10.1128\/CMR.00036-08","article-title":"AmpC beta-lactamases","volume":"22","author":"Jacoby, G.A","year":"2009","unstructured":"Jacoby, G.A. 2009. AmpC beta-lactamases. Clin. Microbiol. Rev. 22:161\u2013182.","journal-title":"Clin. Microbiol. Rev"},{"key":"e_1_3_6_10_2","doi-asserted-by":"crossref","first-page":"417","DOI":"10.3389\/fmicb.2016.00417","article-title":"Escherichia coli beta-lactamases: what really matters","volume":"7","author":"Bajaj P.","year":"2016","unstructured":"Bajaj P., , Singh N.S, , and Virdi J.S.. 2016. Escherichia coli beta-lactamases: what really matters. Front. Microbiol. 7:417.","journal-title":"Front. 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