{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T19:30:05Z","timestamp":1777923005155,"version":"3.51.4"},"reference-count":24,"publisher":"SAGE Publications","issue":"10","license":[{"start":{"date-parts":[[2020,9,28]],"date-time":"2020-09-28T00:00:00Z","timestamp":1601251200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Microbial Drug Resistance"],"published-print":{"date-parts":[[2020,10]]},"abstract":"\n Objectives:<\/jats:title>\n \n This study evaluated the diagnostic performance of the eazyplex\n \u00ae<\/jats:sup>\n SuperBug CRE (eSBCRE) system, based on a loop-mediated isothermal amplification (LAMP), for the detection of the most common extended-spectrum beta-lactamases (ESBL) and carbapenemase genes in 140 clinical isolates of\n Escherichia coli<\/jats:italic>\n .\n <\/jats:p>\n <\/jats:sec>\n \n Materials and Methods:<\/jats:title>\n \n ESBL (\n bla<\/jats:italic>\n CTX-M-1group<\/jats:sub>\n and\n bla<\/jats:italic>\n CTX-M-9group<\/jats:sub>\n ) and carbapenemase (\n bla<\/jats:italic>\n KPC<\/jats:sub>\n ,\n bla<\/jats:italic>\n VIM<\/jats:sub>\n ,\n bla<\/jats:italic>\n NDM<\/jats:sub>\n ,\n bla<\/jats:italic>\n OXA-48<\/jats:sub>\n , and\n bla<\/jats:italic>\n OXA-181<\/jats:sub>\n ) genes were detected using the eSBCRE test and compared with the results obtained by PCR, real-time PCR, and phenotypic methods.\n <\/jats:p>\n <\/jats:sec>\n \n Results:<\/jats:title>\n \n Concordant results of 100% between PCR\/real-time PCR and eSBCRE assays were observed. Two of 140\n E. coli<\/jats:italic>\n isolates were positive for both ESBL and carbapenemase genes according to eSBCRE, PCR, and real-time PCR assays, whereas they were negative in double-disk synergy test. Of 16\n E. coli<\/jats:italic>\n isolates suspected of producing carbapenemase, 9 were positive for 48-oxacillinases (OXA-48) by 30\u2009\u03bcg temocillin test, whereas the\n bla<\/jats:italic>\n OXA-48<\/jats:sub>\n was found only in 1\n E. coli<\/jats:italic>\n isolate by all molecular methods. Maximum threshold time values (minutes:seconds) in the eSBCRE test were 6:00, 11:15, 11:00, and 9:00 for the\n bla<\/jats:italic>\n CTX-M-1group<\/jats:sub>\n ,\n bla<\/jats:italic>\n CTX-M-9group<\/jats:sub>\n ,\n bla<\/jats:italic>\n VIM<\/jats:sub>\n , and\n bla<\/jats:italic>\n OXA-48<\/jats:sub>\n genes, respectively.\n <\/jats:p>\n <\/jats:sec>\n \n Conclusions:<\/jats:title>\n \n The eSBCRE test based on LAMP method is a reliable, easy-to-use, and timesaving molecular system, which can be successfully used in the routine diagnostic for the rapid detection of the most common ESBL and carbapenemase genes among clinical\n E. coli<\/jats:italic>\n isolates.\n <\/jats:p>\n <\/jats:sec>","DOI":"10.1089\/mdr.2019.0311","type":"journal-article","created":{"date-parts":[[2020,1,9]],"date-time":"2020-01-09T11:14:00Z","timestamp":1578568440000},"page":"1245-1249","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":7,"title":["Rapid Detection of Genes Encoding Extended-Spectrum Beta-Lactamase and Carbapenemase in Clinical\n Escherichia coli<\/i>\n Isolates with eazyplex SuperBug CRE System"],"prefix":"10.1177","volume":"26","author":[{"given":"Patrycja","family":"Zalas-Wi\u0119cek","sequence":"first","affiliation":[{"name":"Department of Microbiology, Ludwik Rydygier Collegium Medicum, Nicolaus Copernicus University, Dr Antoni Jurasz University Hospital No 1, Bydgoszcz, Poland."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eugenia","family":"Gospodarek-Komkowska","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Ludwik Rydygier Collegium Medicum, Nicolaus Copernicus University, Dr Antoni Jurasz University Hospital No 1, Bydgoszcz, Poland."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Agata","family":"Smalczewska","sequence":"additional","affiliation":[{"name":"Student Research Club at Department of Microbiology, Ludwik Rydygier Collegium Medicum, Nicolaus Copernicus University, Bydgoszcz, Poland."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2020,9,28]]},"reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"crossref","first-page":"437","DOI":"10.1093\/femsre\/fuw005","article-title":"Escherichia coli: an old friend with new tidings","volume":"40","author":"Vila J.","year":"2016","unstructured":"Vila J., , S\u00e1ez-L\u00f3pez E., , Johnson J.R, , R\u00f6mling U., , Dobrindt U., , Cant\u00f3n R., , Giske C.G, , Naas T., , Carattoli A., , Mart\u00ednez-Medina M., , Bosch J., , Retamar P., , Rodr\u00edguez-Ba\u00f1o J., , Baquero F., , and Soto S.M.. 2016. 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