{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T19:30:06Z","timestamp":1777923006945,"version":"3.51.4"},"reference-count":56,"publisher":"SAGE Publications","issue":"12","license":[{"start":{"date-parts":[[2020,12,1]],"date-time":"2020-12-01T00:00:00Z","timestamp":1606780800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Microbial Drug Resistance"],"published-print":{"date-parts":[[2020,12,1]]},"abstract":"<jats:p>\n                    The aim of this work was to determine the susceptibility, molecular profile, and clonal relationship in\n                    <jats:italic toggle=\"yes\">Streptococcus agalactiae<\/jats:italic>\n                    (group B\n                    <jats:italic toggle=\"yes\">Streptococcus<\/jats:italic>\n                    [GBS]) isolated from vaginal-rectal swab samples. We worked with 200 isolates collected from pregnant women between 35 and 37 weeks of gestation. The macrolide\u2013lincosamide\u2013streptogramin B (MLS\n                    <jats:sub>B<\/jats:sub>\n                    ) resistance phenotypes were determined using the double-disc assay. Susceptibility to erythromycin (ERI) and clindamycin (CLI) was performed with the E-test. Resistance genes\n                    <jats:italic toggle=\"yes\">ermB<\/jats:italic>\n                    and\n                    <jats:italic toggle=\"yes\">ermTR<\/jats:italic>\n                    were detected by polymerase chain reaction. Clonal studies were performed using the random amplification of polymorphic DNA. Twelve (6%) of the isolates were resistant to ERI and 10 (5%) of them to CLI. Fifty percent of the resistant strains corresponded to serotype III, 25% to serotype V, and the remaining 25% to serotype Ia, II, and nontypeable strains. The cMLS\n                    <jats:sub>B<\/jats:sub>\n                    phenotype was detected in eight strains (66.67%) and the iMLS\n                    <jats:sub>B<\/jats:sub>\n                    phenotype in four (33.33%). The minimum inhibitory concentration values were between 1.5 and 16\u2009\u03bcg\/mL for ERI, and between 1 and 32\u2009\u03bcg\/mL for CLI. Out of the 25 strains susceptible to ERI and CLI, the presence of the\n                    <jats:italic toggle=\"yes\">ermB<\/jats:italic>\n                    gene was detected in eight of them and the\n                    <jats:italic toggle=\"yes\">ermTR<\/jats:italic>\n                    gene in one strain. The\n                    <jats:italic toggle=\"yes\">ermB<\/jats:italic>\n                    gene was detected in the 12 strains that initially had some macrolide resistance phenotype. The\n                    <jats:italic toggle=\"yes\">ermTR<\/jats:italic>\n                    gene was detected in three out of the four strains with the iMLS\n                    <jats:sub>B<\/jats:sub>\n                    phenotype. The resistance to macrolides in the province of Misiones is due to multiclonal spread. The phenotypic and genotypic characterization of macrolide resistance in GBS strains are crucial to contribute to the correct intrapartum prophylactic antibiotic therapy of allergic pregnant women and the epidemiological surveillance of these strains.\n                  <\/jats:p>","DOI":"10.1089\/mdr.2019.0328","type":"journal-article","created":{"date-parts":[[2020,4,21]],"date-time":"2020-04-21T17:37:57Z","timestamp":1587490677000},"page":"1472-1481","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":5,"title":["Phenotypic and Genotypic Characterization of Resistance to Macrolides and Lincosamides in\n                    <i>Streptococcus agalactiae<\/i>\n                    Isolated from Pregnant Women in Misiones, Argentina"],"prefix":"10.1177","volume":"26","author":[{"given":"Marina","family":"Novosak","sequence":"first","affiliation":[{"name":"C\u00e1tedra de Bacteriolog\u00eda, Departamento de Microbiolog\u00eda, Facultad de Ciencias Exactas, Qu\u00edmicas y Naturales (FCEQyN), Universidad Nacional de Misiones (UNaM), Misiones, Argentina."},{"name":"Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas (CONICET), Buenos Aires, Argentina."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fernando","family":"Bobadilla","sequence":"additional","affiliation":[{"name":"C\u00e1tedra de Bacteriolog\u00eda, Departamento de Microbiolog\u00eda, Facultad de Ciencias Exactas, Qu\u00edmicas y Naturales (FCEQyN), Universidad Nacional de Misiones (UNaM), Misiones, Argentina."},{"name":"Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas (CONICET), Buenos Aires, Argentina."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Osvaldo","family":"Delgado","sequence":"additional","affiliation":[{"name":"Planta Piloto de Procesos Industriales Microbiol\u00f3gicos (PROIMI-CONICET), Tucum\u00e1n, Argentina."},{"name":"Centro de Biolog\u00eda Molecular y Biotecnolog\u00eda (CEBIOTEC), Facultad de Ciencias Exactas y Naturales (FACEN), Universidad Nacional de Catamarca (UNCa), Catamarca, Argentina."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marta","family":"Vergara","sequence":"additional","affiliation":[{"name":"C\u00e1tedra de Bacteriolog\u00eda, Departamento de Microbiolog\u00eda, Facultad de Ciencias Exactas, Qu\u00edmicas y Naturales (FCEQyN), Universidad Nacional de Misiones (UNaM), Misiones, Argentina."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Margarita","family":"Laczeski","sequence":"additional","affiliation":[{"name":"C\u00e1tedra de Bacteriolog\u00eda, Departamento de Microbiolog\u00eda, Facultad de Ciencias Exactas, Qu\u00edmicas y Naturales (FCEQyN), Universidad Nacional de Misiones (UNaM), Misiones, Argentina."},{"name":"Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas (CONICET), Buenos Aires, Argentina."},{"name":"Laboratorio de Biotecnolog\u00eda Molecular, Instituto de Biotecnolog\u00eda Misiones \u201cDra. Mar\u00eda Ebe Reca\u201d (InBioMis), FCEQyN, UNaM, Misiones, Argentina."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2020,12,3]]},"reference":[{"key":"e_1_3_3_2_2","unstructured":"Baker C. 2000. Group B streptococcal infections. In E. Kaplan and D. Stevens (ed.) Streptococcal infections. clinical aspects microbiology and molecular pathogenesis. 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