{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T19:30:06Z","timestamp":1777923006840,"version":"3.51.4"},"reference-count":62,"publisher":"SAGE Publications","issue":"9","license":[{"start":{"date-parts":[[2020,9,1]],"date-time":"2020-09-01T00:00:00Z","timestamp":1598918400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Microbial Drug Resistance"],"published-print":{"date-parts":[[2020,9,1]]},"abstract":"<jats:p>\n                    Oxacillinases (OXA) have been mostly described in Enterobacteriaceae,\n                    <jats:italic toggle=\"yes\">Acinetobacter<\/jats:italic>\n                    , and\n                    <jats:italic toggle=\"yes\">Pseudomonas<\/jats:italic>\n                    species. Recent years have witnessed an increased prevalence of intrinsic and\/or acquired \u03b2-lactamase-producing\n                    <jats:italic toggle=\"yes\">Acinetobacter<\/jats:italic>\n                    in food-producing animals. This study was conducted to assess the prevalence of OXA among selected bacterial species and to characterize these enzymes by\n                    <jats:italic toggle=\"yes\">in silico<\/jats:italic>\n                    analysis. Screening of\n                    <jats:sc>OXA<\/jats:sc>\n                    was performed by PCR amplification using specific pairs of oligonucleotides. Overall, 40 pairs of primers were designed, of which 6 were experimentally tested\n                    <jats:italic toggle=\"yes\">in vitro<\/jats:italic>\n                    . Among 49 bacterial isolates examined, the presence of\n                    <jats:italic toggle=\"yes\">bla<\/jats:italic>\n                    <jats:sub>OXA-1-like<\/jats:sub>\n                    genes was confirmed in 20 cases (41%; 19 times in\n                    <jats:italic toggle=\"yes\">Klebsiella pneumoniae<\/jats:italic>\n                    and once in\n                    <jats:italic toggle=\"yes\">Enterobacter cloacae<\/jats:italic>\n                    ). No\n                    <jats:sc>OXA<\/jats:sc>\n                    were found in animal isolates. The study results confirmed the specificity of the designed oligonucleotide pairs. Furthermore, the designed primers were found to possess the ability to specifically detect 90.2% of all\n                    <jats:sc>OXA<\/jats:sc>\n                    . These facts suggest that the\n                    <jats:italic toggle=\"yes\">in silico<\/jats:italic>\n                    and\n                    <jats:italic toggle=\"yes\">in vitro<\/jats:italic>\n                    tested primers could be used for single or multiplex PCR to screen for the presence of\n                    <jats:sc>OXA<\/jats:sc>\n                    in various bacteria, as well as to monitor their spread. At the same time, the presence of conserved characteristic amino acids and motifs was confirmed by\n                    <jats:italic toggle=\"yes\">in silico<\/jats:italic>\n                    analysis of sequences of representative members of\n                    <jats:sc>OXA<\/jats:sc>\n                    .\n                  <\/jats:p>","DOI":"10.1089\/mdr.2019.0330","type":"journal-article","created":{"date-parts":[[2020,3,26]],"date-time":"2020-03-26T11:47:42Z","timestamp":1585223262000},"page":"1023-1037","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":13,"title":["PCR Detection of Oxacillinases in Bacteria"],"prefix":"10.1177","volume":"26","author":[{"given":"Patrik","family":"Mlynarcik","sequence":"first","affiliation":[{"name":"Department of Microbiology, Faculty of Medicine and Dentistry, Palacky University Olomouc, Olomouc, Czech Republic."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andrea","family":"Chalachanova","sequence":"additional","affiliation":[{"name":"Department of Cell Biology and Genetics, Faculty of Science, Palacky University Olomouc, Olomouc, Czech Republic."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Iva","family":"Vagnerov\u0103","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Faculty of Medicine and Dentistry, Palacky University Olomouc, Olomouc, Czech Republic."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ondrej","family":"Holy","sequence":"additional","affiliation":[{"name":"Department of Public Health, Faculty of Medicine and Dentistry, Palacky University Olomouc, Olomouc, Czech Republic."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Simona","family":"Zatloukalova","sequence":"additional","affiliation":[{"name":"Department of Public Health, Faculty of Medicine and Dentistry, Palacky University Olomouc, Olomouc, Czech Republic."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Milan","family":"Kolar","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Faculty of Medicine and Dentistry, Palacky University Olomouc, Olomouc, Czech Republic."},{"name":"Institute of Molecular and Translational Medicine, Faculty of Medicine and Dentistry, Palacky University Olomouc, Olomouc, Czech Republic."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2020,9,9]]},"reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1093\/clinids\/10.4.681"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01512-08"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.2174\/1381612805666230112185101"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01483-13"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.48.6.2043-2048.2004"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-1033.1989.tb14649.x"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01517-09"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01132-06"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.48.1.15-22.2004"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00116-06"},{"key":"e_1_3_3_12_2","first-page":"located","article-title":"First detection of the OXA-40 carbapenemase in P","author":"Sevillano E.","year":"2009","unstructured":"Sevillano E., , Gallego L., , and Garcia-Lobo J.M.. 2009. 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