{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T19:30:07Z","timestamp":1777923007891,"version":"3.51.4"},"reference-count":20,"publisher":"SAGE Publications","issue":"9","license":[{"start":{"date-parts":[[2020,9,1]],"date-time":"2020-09-01T00:00:00Z","timestamp":1598918400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Microbial Drug Resistance"],"published-print":{"date-parts":[[2020,9,1]]},"abstract":"<jats:sec>\n                    <jats:title>Background:<\/jats:title>\n                    <jats:p>\n                      The aim of this study was to investigate the characteristics of carbapenem-resistant\n                      <jats:italic toggle=\"yes\">Klebsiella pneumoniae<\/jats:italic>\n                      (CRKP) ST76 isolates collected during an outbreak in a hospital's intensive care unit and neurosurgery unit.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods:<\/jats:title>\n                    <jats:p>Seventeen separate clinical isolates of CRKP were collected from patients from March 2016 to February 2017. Bacterial isolates were identified, and antimicrobial susceptibility testing was conducted using the VITEK-2 compact system. Isolates containing antibiotic resistance genes were characterized by polymerase chain reaction and DNA sequencing. Clonal relatedness was assessed by multilocus sequence typing and pulsed-field gel electrophoresis. Conjugation experiments were performed to determine the transferability of plasmids with antibiotic resistance. The genomic features and mobile genetic elements of ST76 CRKP were detected by whole genome sequencing.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results:<\/jats:title>\n                    <jats:p>\n                      ST76 KPC-2\u2013producing CRKP prevailed in our hospital, causing an outbreak. The strains also carried\n                      <jats:italic toggle=\"yes\">bla<\/jats:italic>\n                      <jats:sub>SHV-1<\/jats:sub>\n                      ,\n                      <jats:italic toggle=\"yes\">bla<\/jats:italic>\n                      <jats:sub>CTX-M-15<\/jats:sub>\n                      ,\n                      <jats:italic toggle=\"yes\">bla<\/jats:italic>\n                      <jats:sub>TEM-1<\/jats:sub>\n                      ,\n                      <jats:italic toggle=\"yes\">qnrB<\/jats:italic>\n                      , and\n                      <jats:italic toggle=\"yes\">acc(6\u2032)Ib-cr<\/jats:italic>\n                      resistance genes. Plasmids from 17.7% of the isolated strains bearing these resistance genes could be transferred into the recipient\n                      <jats:italic toggle=\"yes\">Escherichia coli<\/jats:italic>\n                      J53 through conjugation. Sequencing results showed that the KP4 genome mainly consisted of a circular chromosome and three antibiotic resistance plasmids. The plasmid carrying the\n                      <jats:italic toggle=\"yes\">bla<\/jats:italic>\n                      <jats:sub>KPC-2<\/jats:sub>\n                      gene was located on a 437\u2009kb IncFIB (pQil) plasmid with Tn\n                      <jats:italic toggle=\"yes\">1721<\/jats:italic>\n                      -\n                      <jats:italic toggle=\"yes\">bla<\/jats:italic>\n                      <jats:sub>KPC-2<\/jats:sub>\n                      -\u0394T\n                      <jats:italic toggle=\"yes\">3<\/jats:italic>\n                      gene structure. Genes conferring resistance against aminoglycosides, quinolones, fluoroquinolones, beta-lactamase, phenicols, sulfonamides, and trimethoprims and the presence of virulence-associated genes related to iron acquisition or adhesins were determined.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion:<\/jats:title>\n                    <jats:p>\n                      This is the first report of the whole genome sequence of a KPC-2\u2013producing\n                      <jats:italic toggle=\"yes\">K. pneumoniae<\/jats:italic>\n                      ST76 isolate. This work provides a basis for understanding antibiotic resistance and resistant plasmid transmission. Relevant departments should implement infection control and prevention measures to reduce the incidence of nosocomial infections.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1089\/mdr.2019.0363","type":"journal-article","created":{"date-parts":[[2020,3,9]],"date-time":"2020-03-09T16:36:45Z","timestamp":1583771805000},"page":"1009-1018","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":17,"title":["Outbreak of KPC-2\u2013Producing\n                    <i>Klebsiella pneumoniae<\/i>\n                    ST76 Isolates in an Intensive Care Unit and Neurosurgery Unit"],"prefix":"10.1177","volume":"26","author":[{"given":"Shanshan","family":"Su","sequence":"first","affiliation":[{"name":"Department of Microbiology, Yongchuan Hospital of Chongqing Medical University, Chongqing, China."},{"name":"Department of Microbiology, The First People's Hospital of Jingzhou City, Jingzhou, China."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chunjiang","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Microbiology, First Affiliated Hospital of Jiamusi University, Jiamusi, China."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yongxin","family":"Zhao","sequence":"additional","affiliation":[{"name":"Department of Microbiology, First Affiliated Hospital of Jiamusi University, Jiamusi, China."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lan","family":"Yu","sequence":"additional","affiliation":[{"name":"Department of Microbiology, First Affiliated Hospital of Jiamusi University, Jiamusi, China."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yong","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Microbiology, First Affiliated Hospital of Jiamusi University, Jiamusi, China."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuchao","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Microbiology, First Affiliated Hospital of Jiamusi University, Jiamusi, China."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mingjia","family":"Bao","sequence":"additional","affiliation":[{"name":"Center for Disease Control and Prevention, Jiamusi, China."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yu","family":"Fu","sequence":"additional","affiliation":[{"name":"Center for Disease Control and Prevention, Jiamusi, China."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jisheng","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Yongchuan Hospital of Chongqing Medical University, Chongqing, China."},{"name":"Department of Microbiology, First Affiliated Hospital of Jiamusi University, Jiamusi, China."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaoli","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Yongchuan Hospital of Chongqing Medical University, Chongqing, China."},{"name":"Department of Microbiology, First Affiliated Hospital of Jiamusi University, Jiamusi, China."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2020,9,9]]},"reference":[{"key":"e_1_3_6_2_2","doi-asserted-by":"publisher","DOI":"10.1099\/jmm.0.036483-0"},{"key":"e_1_3_6_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01397-14"},{"key":"e_1_3_6_4_2","doi-asserted-by":"publisher","DOI":"10.2147\/IDR.S212333"},{"key":"e_1_3_6_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.45.4.1151-1161.2001"},{"key":"e_1_3_6_6_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/jiw282"},{"key":"e_1_3_6_7_2","volume-title":"Klebsiella pneumoniae","author":"Dong N.","unstructured":"Dong N., , Zhang R., , Liu L., , Li R., , Lin D., , Chan E.W, , and Chen S.. 2018. Genome analysis of clinical multilocus sequence Type 11 Klebsiella pneumoniae. from China. Microb. Genom. 4: e00, 0149."},{"key":"e_1_3_6_8_2","doi-asserted-by":"publisher","DOI":"10.1111\/apm.12433"},{"key":"e_1_3_6_9_2","doi-asserted-by":"publisher","DOI":"10.1089\/mdr.2018.0014"},{"key":"e_1_3_6_10_2","volume-title":"Klebsiella pneumoniae","author":"Zhang X.","unstructured":"Zhang X., , Chen D., , Xu G., , Huang W., , and Wang X.. 2018. Molecular epidemiology and drug resistant mechanism in carbapenem-resistant Klebsiella pneumoniae. isolated from pediatric patients in Shanghai, China. 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