{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,19]],"date-time":"2026-05-19T20:19:13Z","timestamp":1779221953583,"version":"3.51.4"},"reference-count":36,"publisher":"SAGE Publications","issue":"7","license":[{"start":{"date-parts":[[2020,7,1]],"date-time":"2020-07-01T00:00:00Z","timestamp":1593561600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Microbial Drug Resistance"],"published-print":{"date-parts":[[2020,7,1]]},"abstract":"<jats:sec>\n                    <jats:title>Objectives:<\/jats:title>\n                    <jats:p>The aim was to assess the potential advantage of combined genotypic testing with phenotypic antimicrobial susceptibility testing (AST) to detect AmpC \u03b2-lactamases (AmpC) and extended-spectrum \u03b2-lactamases (ESBL) producing Enterobacteriaceae isolated from blood cultures in a pediatric population.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Materials and Methods:<\/jats:title>\n                    <jats:p>All first-time Enterobacteriaceae isolates recovered from blood cultures of pediatric patients at the Cincinnati Children's Hospital Medical Center between January 2017 and December 2018 were evaluated. The Check-MDR CT103XL \u03b2-lactamase assay was used to determine the presence of AmpC and ESBL, while AST was performed using the VITEK 2 platform. Phenotypic ESBL resistance was defined by resistance to either ceftriaxone or ceftazidime using Clinical and Laboratory Standards Institute breakpoints, while combined cefoxitin resistance with ceftriaxone or ceftazidime resistance was used to detect AmpCs (as per European Committee on Antimicrobial Susceptibility Testing standards).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results:<\/jats:title>\n                    <jats:p>Overall, there were 170 isolates. Genotypically, 21 (12.4%) had AmpC and 18 (10.6%) had ESBL genes detected. Phenotypically, 11 (6.5%) isolates were AmpC and 26 (15.3%) were ESBL producing organisms. Genotypic testing identified an additional 14 AmpC and two ESBL isolates that failed to meet phenotypic criteria.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions:<\/jats:title>\n                    <jats:p>Using combined genotypic and phenotypic methods to detect AmpC and ESBL producing organisms increased the identification of resistant organism and provided potentially clinical relevant data to guide the treatment of resistant organisms.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1089\/mdr.2019.0432","type":"journal-article","created":{"date-parts":[[2020,3,17]],"date-time":"2020-03-17T09:58:12Z","timestamp":1584439092000},"page":"825-830","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":2,"title":["Combined Molecular and Phenotypic Antimicrobial Susceptibility Testing Is Beneficial in Detection of ESBL and AmpC Beta-Lactamase Producing Isolates of Enterobacteriaceae in Pediatric Patients with Bloodstream Infections"],"prefix":"10.1177","volume":"26","author":[{"given":"Eleanor A.","family":"Powell","sequence":"first","affiliation":[{"name":"Department of Pathology and Laboratory Medicine, Cincinnati Children's Hospital Medical Center (CCHMC), Cincinnati, Ohio, USA."},{"name":"Department of Pathology and Laboratory Medicine, University of Cincinnati, Cincinnati, Ohio, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nadim","family":"Khalil","sequence":"additional","affiliation":[{"name":"Division of Pediatric Infectious Diseases, CCHMC, Cincinnati, Ohio, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Barbara","family":"DeBurger","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine, Cincinnati Children's Hospital Medical Center (CCHMC), Cincinnati, Ohio, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joel E.","family":"Mortensen","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine, Cincinnati Children's Hospital Medical Center (CCHMC), Cincinnati, Ohio, USA."},{"name":"Department of Pathology and Laboratory Medicine, University of Cincinnati, Cincinnati, Ohio, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2020,7,7]]},"reference":[{"key":"e_1_3_6_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijid.2006.07.008"},{"key":"e_1_3_6_3_2","first-page":"161","volume":"19","author":"Matsumura Y.","year":"2013","unstructured":"Matsumura Y., , Nagao M., , Iguchi M., , Yagi T., , Komori T., , Fujita N., , Yamamoto M., , Matsushima A., , Takakura S., , and Ichiyama S.. 2013. Molecular and clinical characterization of plasmid-mediated AmpC \u03b2-lactamase-producing Escherichia coli bacteraemia: a comparison with extended-spectrum \u03b2-lactamase-producing and non-resistant E. coli bacteraemia. Clin. Microbiol. Infect. 19:161\u2013168.","journal-title":"Molecular and clinical characterization of plasmid-mediated AmpC \u03b2-lactamase-producing Escherichia coli bacteraemia: a comparison with extended-spectrum \u03b2-lactamase-producing and non-resistant E. coli bacteraemia. Clin. Microbiol. Infect"},{"key":"e_1_3_6_4_2","doi-asserted-by":"publisher","DOI":"10.1086\/420816"},{"key":"e_1_3_6_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00036-08"},{"key":"e_1_3_6_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.46.5.1481-1491.2002"},{"key":"e_1_3_6_7_2","doi-asserted-by":"publisher","DOI":"10.1186\/s13104-018-3729-3"},{"key":"e_1_3_6_8_2","unstructured":"Clinical Laboratory Standards Institute. 2019. M100-Performance Standards for Antimicrobial Susceptibility Testing. 29th ed. Clinical and Laboratory Standards Institute Wayne PA."},{"key":"e_1_3_6_9_2","unstructured":"The European Committee on Antimicrobial Susceptibility Testing. Breakpoint tables for interpretation of MICs and zone diameters. Version 9.0 [Internet]. 2019. Available from: www.eucast.org\/fileadmin\/src\/media\/PDFs\/EUCAST_files\/Breakpoint_tables\/v_9.0_Breakpoint_Tables.pdf"},{"key":"e_1_3_6_10_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkt136"},{"key":"e_1_3_6_11_2","unstructured":"Clinical Laboratory Standards Institute. M100-Performance Standards for Antimicrobial Susceptibility Testing. 19th ed. Clinical and Laboratory Standards Institute; 2009."},{"key":"e_1_3_6_12_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-0691.2011.03703.x"},{"key":"e_1_3_6_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmi.2019.04.025"},{"key":"e_1_3_6_14_2","doi-asserted-by":"publisher","DOI":"10.1086\/647952"},{"key":"e_1_3_6_15_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.02130-10"},{"key":"e_1_3_6_16_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6976.2011.00268.x"},{"key":"e_1_3_6_17_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00126-09"},{"key":"e_1_3_6_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.clinthera.2016.11.025"},{"key":"e_1_3_6_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.diagmicrobio.2017.04.011"},{"key":"e_1_3_6_20_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12941-016-0168-7"},{"key":"e_1_3_6_21_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dks156"},{"key":"e_1_3_6_22_2","first-page":"220","volume":"10","author":"Sanders W.E.","year":"1997","unstructured":"Sanders W.E., , and Sanders C.C.. 1997. Enterobacter spp.: pathogens poised to flourish at the turn of the century. Clin. Microbiol. Rev. 10:220\u2013241.","journal-title":"Enterobacter spp.: pathogens poised to flourish at the turn of the century. Clin. Microbiol. Rev"},{"key":"e_1_3_6_23_2","doi-asserted-by":"publisher","DOI":"10.1186\/s13756-017-0177-0"},{"key":"e_1_3_6_24_2","doi-asserted-by":"publisher","DOI":"10.7326\/0003-4819-115-8-585"},{"key":"e_1_3_6_25_2","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/ciu182"},{"key":"e_1_3_6_26_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.48.10.3720-3728.2004"},{"key":"e_1_3_6_27_2","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/cis916"},{"key":"e_1_3_6_28_2","doi-asserted-by":"publisher","DOI":"10.1001\/jama.2018.12163"},{"key":"e_1_3_6_29_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dks301"},{"key":"e_1_3_6_30_2","doi-asserted-by":"publisher","DOI":"10.1093\/ofid\/ofw132"},{"key":"e_1_3_6_31_2","doi-asserted-by":"publisher","DOI":"10.1099\/jmm.0.068981-0"},{"key":"e_1_3_6_32_2","doi-asserted-by":"publisher","DOI":"10.1089\/mdr.2017.0018"},{"key":"e_1_3_6_33_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.43.8.1924"},{"key":"e_1_3_6_34_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.diagmicrobio.2017.04.006"},{"key":"e_1_3_6_35_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.2002.tb11164.x"},{"key":"e_1_3_6_36_2","first-page":"e0150643","article-title":"Genotypic and phenotypic detection of AmpC \u03b2-lactamases in Enterobacter spp","volume":"11","author":"Khari F.I.M.","year":"2016","unstructured":"Khari F.I.M., , Karunakaran R., , Rosli R., , and Tay S.T.. 2016. Genotypic and phenotypic detection of AmpC \u03b2-lactamases in Enterobacter spp. isolated from a teaching hospital in Malaysia. PLoS One, 11:e0150643.","journal-title":"isolated from a teaching hospital in Malaysia. PLoS One"},{"key":"e_1_3_6_37_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.02697-14"}],"container-title":["Microbial Drug Resistance"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/full-xml\/10.1089\/mdr.2019.0432","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1089\/mdr.2019.0432","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1089\/mdr.2019.0432","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T20:37:01Z","timestamp":1777667821000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1089\/mdr.2019.0432"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,7,1]]},"references-count":36,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2020,7,1]]}},"alternative-id":["10.1089\/mdr.2019.0432"],"URL":"https:\/\/doi.org\/10.1089\/mdr.2019.0432","relation":{},"ISSN":["1076-6294","1931-8448"],"issn-type":[{"value":"1076-6294","type":"print"},{"value":"1931-8448","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,7,1]]}}}