{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,11]],"date-time":"2026-10-11T13:46:36Z","timestamp":1791726396158,"version":"4.3.4"},"reference-count":79,"publisher":"SAGE Publications","issue":"11","license":[{"start":{"date-parts":[[2021,11,1]],"date-time":"2021-11-01T00:00:00Z","timestamp":1635724800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Microbial Drug Resistance"],"published-print":{"date-parts":[[2021,11,1]]},"abstract":"<jats:p>\n                    This study describes comparative occurrence and characterization of multidrug-resistant (MDR)\n                    <jats:italic toggle=\"yes\">Escherichia coli<\/jats:italic>\n                    and\n                    <jats:italic toggle=\"yes\">Klebsiella pneumoniae<\/jats:italic>\n                    (KP) in healthy cattle (HC) and cattle with diarrhea (DC) in India. During 2018\u20132020, 72 MDR isolates, including 35\n                    <jats:italic toggle=\"yes\">E. coli<\/jats:italic>\n                    (DC: 27; HC 8) and 37\n                    <jats:italic toggle=\"yes\">K. pneumoniae<\/jats:italic>\n                    (DC: 34; HC: 3), from 251 rectal swabs (DC: 219; HC: 32) were investigated for extended-spectrum beta-lactamase (ESBL), AmpC type \u03b2-lactamase and carbapenemase production, antimicrobial susceptibility profile, biofilm production, and efflux pump activity. Fifty-five MDR isolates were ESBL producers (ESBLPs) (DC: 50; HC: 5) and ESBLPs from DC were coresistant to multiple antibiotics. The\n                    <jats:italic toggle=\"yes\">bla<\/jats:italic>\n                    CTX-M gene (50) was the most frequently detected \u03b2-lactamases followed by\n                    <jats:italic toggle=\"yes\">bla<\/jats:italic>\n                    AmpC (22),\n                    <jats:italic toggle=\"yes\">bla<\/jats:italic>\n                    TEM1 (13),\n                    <jats:italic toggle=\"yes\">bla<\/jats:italic>\n                    CMY-6 (6),\n                    <jats:italic toggle=\"yes\">bla<\/jats:italic>\n                    OXA1 (5),\n                    <jats:italic toggle=\"yes\">bla<\/jats:italic>\n                    PER (2),\n                    <jats:italic toggle=\"yes\">bla<\/jats:italic>\n                    DHA, and\n                    <jats:italic toggle=\"yes\">bla<\/jats:italic>\n                    FOX and\n                    <jats:italic toggle=\"yes\">bla<\/jats:italic>\n                    SHV12 (1 each). Plasmid-mediated quinolone resistance determinants\n                    <jats:italic toggle=\"yes\">qnrB<\/jats:italic>\n                    ,\n                    <jats:italic toggle=\"yes\">qnrS<\/jats:italic>\n                    ,\n                    <jats:italic toggle=\"yes\">qnrA<\/jats:italic>\n                    , and\n                    <jats:italic toggle=\"yes\">qepA<\/jats:italic>\n                    were detected in 18, 16, 2, and 3 isolates, respectively. Twenty three isolates revealed mutation in\n                    <jats:italic toggle=\"yes\">gyrA<\/jats:italic>\n                    and\n                    <jats:italic toggle=\"yes\">parC<\/jats:italic>\n                    genes. Tetracycline-resistance markers\n                    <jats:italic toggle=\"yes\">tetA<\/jats:italic>\n                    ,\n                    <jats:italic toggle=\"yes\">tetB<\/jats:italic>\n                    ,\n                    <jats:italic toggle=\"yes\">tetC<\/jats:italic>\n                    , and\n                    <jats:italic toggle=\"yes\">tetE<\/jats:italic>\n                    were detected in 33, 10, 3, and 2 isolates, respectively. Only one of the 41 imipenem-resistant isolates harbored\n                    <jats:italic toggle=\"yes\">bla<\/jats:italic>\n                    NDM-5 and two were colistin-resistant. Altogether, 20 MDR isolates were strong biofilm producers and 19 harbored different virulence factors. This is the first ever report from India on the presence of MDR Enterobacteriaceae with resistance to even last-resort antimicrobials in the bovine diarrhea.\n                  <\/jats:p>","DOI":"10.1089\/mdr.2020.0298","type":"journal-article","created":{"date-parts":[[2021,4,29]],"date-time":"2021-04-29T11:02:50Z","timestamp":1619694170000},"page":"1457-1469","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":23,"title":["Characterization of Multidrug-Resistant Biofilm-Producing\n                    <i>Escherichia coli<\/i>\n                    and\n                    <i>Klebsiella pneumoniae<\/i>\n                    in Healthy Cattle and Cattle with Diarrhea"],"prefix":"10.1177","volume":"27","author":[{"given":"Samiran","family":"Bandyopadhyay","sequence":"first","affiliation":[{"name":"ICAR-Indian Veterinary Research Institute, Eastern Regional Station, Kolkata, India."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Debaraj","family":"Bhattacharyya","sequence":"additional","affiliation":[{"name":"ICAR-Indian Veterinary Research Institute, Eastern Regional Station, Kolkata, India."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Indranil","family":"Samanta","sequence":"additional","affiliation":[{"name":"Department of Veterinary Microbiology, Faculty of Veterinary and Animal Sciences, West Bengal University of Animal and Fishery Sciences, Kolkata, India."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jaydeep","family":"Banerjee","sequence":"additional","affiliation":[{"name":"ICAR-Indian Veterinary Research Institute, Eastern Regional Station, Kolkata, India."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Md","family":"Habib","sequence":"additional","affiliation":[{"name":"ICAR-Indian Veterinary Research Institute, Eastern Regional Station, Kolkata, India."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tapan K.","family":"Dutta","sequence":"additional","affiliation":[{"name":"C.V.Sc. &amp; A.H, Central Agricultural University, Aizawl, India."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Triveni","family":"Dutt","sequence":"additional","affiliation":[{"name":"Division of Livestock Production and Management, ICAR\u2014Indian Veterinary Research Institute, Izatnagar, India."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2021,11,11]]},"reference":[{"key":"e_1_3_5_2_2","first-page":"2050","article-title":"Global perspective studies team","volume":"2030","author":"Alexandratos N.","year":"2012","unstructured":"Alexandratos N., , and Bruinsma J.. 2012. 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