{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T19:15:28Z","timestamp":1790709328954,"version":"4.1.0"},"reference-count":41,"publisher":"SAGE Publications","issue":"12","license":[{"start":{"date-parts":[[2021,12,1]],"date-time":"2021-12-01T00:00:00Z","timestamp":1638316800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Microbial Drug Resistance"],"published-print":{"date-parts":[[2021,12,1]]},"abstract":"<jats:p>\n                    The development of carbapenem resistance in extraintestinal pathogenic\n                    <jats:italic toggle=\"yes\">Escherichia coli<\/jats:italic>\n                    (ExPEC) has significant clinical implications, particularly in countries where second-line antimicrobials are not readily available, rendering treatments ineffective, and ExPEC infections untreatable. Thus, early detection of high-risk ExPEC lineages and raising awareness of the specific mechanisms underlying carbapenem resistance are mandatory for the selection of appropriate treatment options and the prevention of\n                    <jats:italic toggle=\"yes\">E. coli<\/jats:italic>\n                    spread. This study aims to investigate the phenotypic and genotypic features of the first NDM-5 carbapenemase-producing ExPEC strain isolated from the blood of a patient admitted to the Maputo Central Hospital (MCH), in Mozambique.\n                    <jats:italic toggle=\"yes\">E. coli<\/jats:italic>\n                    SSM100 isolate was identified by MALDI-TOF, it displayed high-level resistance to third generation cephalosporins, carbapenems, fluoroquinolones, and aminoglycosides, performing antimicrobial susceptibilities testing by VITEK 2 system.\n                    <jats:italic toggle=\"yes\">E. coli<\/jats:italic>\n                    SSM100 isolate was classified through whole-genome sequencing as ST405-D-O102: H6, a globally distributed lineage associated with antimicrobial resistance, carrying the\n                    <jats:italic toggle=\"yes\">bla<\/jats:italic>\n                    NDM-5 gene located on an F1:A1:B49 plasmid, coharboring\n                    <jats:italic toggle=\"yes\">bla<\/jats:italic>\n                    CTX-M-15,\n                    <jats:italic toggle=\"yes\">bla<\/jats:italic>\n                    TEM-1,\n                    <jats:italic toggle=\"yes\">aadA2<\/jats:italic>\n                    ,\n                    <jats:italic toggle=\"yes\">sul1<\/jats:italic>\n                    , and\n                    <jats:italic toggle=\"yes\">dfrA12<\/jats:italic>\n                    genes. In addition, mutations in\n                    <jats:italic toggle=\"yes\">gyrA<\/jats:italic>\n                    (S83L and D87N),\n                    <jats:italic toggle=\"yes\">parC<\/jats:italic>\n                    (S80I and E84V), and\n                    <jats:italic toggle=\"yes\">parE<\/jats:italic>\n                    (I529L) conferring fluoroquinolone resistance were also found. Moreover, SSM100 isolate carried 88 virulence genes, of which 28 are reported to be associated with UPEC. The emergence of NDM-5 carbapenemase in a pandemic ST405-D-O102:H6 clone in Mozambique is of great concern. Locations of extended-spectrum \u03b2-lactamase determinants and NDM-5 carbapenemase gene on Inc\n                    <jats:italic toggle=\"yes\">F<\/jats:italic>\n                    -plasmid can increase their spread reinforcing the need for antimicrobial surveillance and the urgent introduction of carbapenemase detection tests in diagnostic laboratories of the country.\n                  <\/jats:p>","DOI":"10.1089\/mdr.2020.0334","type":"journal-article","created":{"date-parts":[[2021,6,2]],"date-time":"2021-06-02T12:13:18Z","timestamp":1622635998000},"page":"1633-1640","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":20,"title":["Extraintestinal Pathogenic\n                    <i>Escherichia coli<\/i>\n                    ST405 Isolate Coharboring blaNDM-5 and blaCTXM-15: A New Threat in Mozambique"],"prefix":"10.1177","volume":"27","author":[{"given":"Jos\u00e9 Jo\u00e3o","family":"Sumbana","sequence":"first","affiliation":[{"name":"Department of Biological Sciences, Eduardo Mondlane University, Maputo, Mozambique."},{"name":"Department of Biomedical Sciences, University of Sassari, Sassari, Italy."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7871-3911","authenticated-orcid":false,"given":"Antonella","family":"Santona","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences, University of Sassari, Sassari, Italy."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maura","family":"Fiamma","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences, University of Sassari, Sassari, Italy."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elisa","family":"Taviani","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences, University of Sassari, Sassari, Italy."},{"name":"Center of Biotechnology, Eduardo Mondlane University, Maputo, Mozambique."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Massimo","family":"Deligios","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences, University of Sassari, Sassari, Italy."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tom\u00e1s","family":"Zimba","sequence":"additional","affiliation":[{"name":"Maputo Central Hospital, Maputo, Mozambique."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gilberto","family":"Lucas","sequence":"additional","affiliation":[{"name":"Maputo Central Hospital, Maputo, Mozambique."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jahit","family":"Sacarlal","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Faculty of Medicine, Eduardo Mondlane University, Maputo, Mozambique."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Salvatore","family":"Rubino","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences, University of Sassari, Sassari, Italy."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bianca","family":"Paglietti","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences, University of Sassari, Sassari, Italy."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2021,12,6]]},"reference":[{"key":"e_1_3_5_2_2","first-page":"e000197","article-title":"Extended-spectrum \u03b2-lactamase-producing and carbapenemase-producing Enterobacteriaceae","volume":"4","author":"Wilson H.","year":"2018","unstructured":"Wilson H., , and T\u00f6r\u00f6k M.E.. 2018. 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