{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,5]],"date-time":"2026-05-05T09:18:27Z","timestamp":1777972707971,"version":"3.51.4"},"reference-count":42,"publisher":"SAGE Publications","issue":"1","license":[{"start":{"date-parts":[[2022,1,1]],"date-time":"2022-01-01T00:00:00Z","timestamp":1640995200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Microbial Drug Resistance"],"published-print":{"date-parts":[[2022,1,1]]},"abstract":"<jats:sec>\n                    <jats:title>Objective:<\/jats:title>\n                    <jats:p>\n                      This study's main objective was to analyze the antibiotic susceptibility profile of\n                      <jats:italic toggle=\"yes\">Escherichia coli<\/jats:italic>\n                      isolates obtained from a fecal sample of a captive Baird's tapir (\n                      <jats:italic toggle=\"yes\">Tapirus bairdii<\/jats:italic>\n                      ) in Costa Rica.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Materials and Methods:<\/jats:title>\n                    <jats:p>\n                      The fecal sample was collected inside the enclosure on March 3, 2017, right after the animal defecated. Samples were cultured on MacConkey agar plates nonsupplemented and supplemented with 2\u2009\u03bcg\/mL of cefotaxime. Bacterial identification and antibiotic susceptibility were performed with the Vitek 2 Compact System and the Kirby Bauer disk diffusion method, respectively. Polymerase chain reaction amplification was performed to detect\n                      <jats:italic toggle=\"yes\">bla<\/jats:italic>\n                      <jats:sub>CTX-M<\/jats:sub>\n                      beta-lactamase genes. Resistant isolates were subjected to whole-genome sequencing (WGS).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results:<\/jats:title>\n                    <jats:p>\n                      After evaluating several antibiotic classes, a multidrug-resistant\n                      <jats:italic toggle=\"yes\">E. coli<\/jats:italic>\n                      strain with extended-spectrum beta-lactamase phenotype was isolated. Resistance to cefotaxime, cefepime, ampicillin, ampicillin\/sulbactam, and tetracycline was detected. WGS analysis showed the presence of\n                      <jats:italic toggle=\"yes\">bla<\/jats:italic>\n                      <jats:sub>CTX-M-1<\/jats:sub>\n                      ,\n                      <jats:italic toggle=\"yes\">bla<\/jats:italic>\n                      <jats:sub>TEM-1B<\/jats:sub>\n                      , and\n                      <jats:italic toggle=\"yes\">tet<\/jats:italic>\n                      (B) genes. The presence of IncN plasmids and Col156 was also detected.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion:<\/jats:title>\n                    <jats:p>Our findings are according with the notion that animals' high density enhances the spread of resistant determinants in a captive environment in a limited space, where the likelihood of direct or indirect contact with other animals and humans is more frequent.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1089\/mdr.2020.0339","type":"journal-article","created":{"date-parts":[[2021,7,27]],"date-time":"2021-07-27T17:30:00Z","timestamp":1627407000000},"page":"143-148","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":6,"title":["First Report of a Multidrug-Resistant ST58\n                    <i>Escherichia coli<\/i>\n                    Harboring Extended-Spectrum Beta-Lactamase of the CTX-M-1 Class in a Fecal Sample of a Captive Baird's Tapir (\n                    <i>Tapirus bairdii<\/i>\n                    ) in Costa Rica, Central America"],"prefix":"10.1177","volume":"28","author":[{"given":"Jorge","family":"Rojas-Jim\u00e9nez","sequence":"first","affiliation":[{"name":"Warnell School of Forestry and Natural Resources, The University of Georgia, Athens, Georgia, USA."},{"name":"Costa Rica Wildlife Foundation, San Jos\u00e9, Costa Rica."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mar\u00eda Antonieta","family":"Jim\u00e9nez-Pearson","sequence":"additional","affiliation":[{"name":"Instituto Costarricense de Investigaci\u00f3n y Ense\u00f1anza en Nutrici\u00f3n y Salud, San Jos\u00e9, Costa Rica."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Francisco","family":"Duarte-Mart\u00ednez","sequence":"additional","affiliation":[{"name":"Instituto Costarricense de Investigaci\u00f3n y Ense\u00f1anza en Nutrici\u00f3n y Salud, San Jos\u00e9, Costa Rica."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Esteban","family":"Brenes-Mora","sequence":"additional","affiliation":[{"name":"Costa Rica Wildlife Foundation, San Jos\u00e9, Costa Rica."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Randall","family":"Arguedas","sequence":"additional","affiliation":[{"name":"Universidad T\u00e9cnica Nacional, Sede Atenas, Costa Rica."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4428-3340","authenticated-orcid":false,"given":"El\u00edas","family":"Barquero-Calvo","sequence":"additional","affiliation":[{"name":"Programa de Investigaci\u00f3n en Enfermedades Tropicales, Escuela de Medicina Veterinaria, Universidad Nacional de Costa Rica, Lagunilla, Costa Rica."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2022,1,13]]},"reference":[{"key":"e_1_3_4_2_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749-4877.2012.00316.x"},{"key":"e_1_3_4_3_2","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.1501105"},{"key":"e_1_3_4_4_2","doi-asserted-by":"publisher","DOI":"10.1111\/btp.12627"},{"key":"e_1_3_4_5_2","doi-asserted-by":"crossref","unstructured":"Garc\u00eda M. Jordan C. O'Farril G. et al. 2016. Tapirus bairdii. The IUCN Red List of Threatened Species. 2016:e.T21471A45173340. Available at https:\/\/dx.doi.org\/10.2305\/IUCN.UK.2016-1.RLTS.T21471A45173340.en (accessed June18 2021).","DOI":"10.2305\/IUCN.UK.2016-1.RLTS.T21471A45173340.en"},{"key":"e_1_3_4_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.biocon.2020.108501"},{"key":"e_1_3_4_7_2","unstructured":"Brenes-Mora E. 2018. Activity patterns habitat selection and knockdowns of tapir (tapirus bairdii) in a forest complex sectioned by a road in the Talamanca mountain range Costa Rica [in Portuguese]. Universidad Nacional de Costa Rica Heredia Costa Rica. Available at http:\/\/hdl.handle.net\/11056\/14856 (accessed June18 2021)."},{"key":"e_1_3_4_8_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749-4877.2012.00323.x"},{"key":"e_1_3_4_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2008.07.022"},{"key":"e_1_3_4_10_2","doi-asserted-by":"publisher","DOI":"10.1111\/zph.12182"},{"key":"e_1_3_4_11_2","first-page":"yet","article-title":"Extended-spectrum beta-lactamases producing E","author":"Guenther S.","year":"2011","unstructured":"Guenther S., , Ewers C., , and Wieler L.H.. 2011. Extended-spectrum beta-lactamases producing E. coli in, wildlife, yet another form of environmental pollution? Front. Microbiol. 2:1\u201313.","journal-title":"coli in"},{"key":"e_1_3_4_12_2","first-page":"648","article-title":"Molecular mechanisms of antibiotic resistance in Escherichia coli\u2014associated diarrhea","volume":"28","author":"Mosquito S.","year":"2011","unstructured":"Mosquito S., , Ruiz J., , Bauer J.L, , and Ochoa T.J.. 2011. Molecular mechanisms of antibiotic resistance in Escherichia coli\u2014associated diarrhea. Rev. Peru. Med. Exp. Salud Publica, 28:648\u2013656.","journal-title":"Rev. Peru. Med. Exp. Salud Publica"},{"key":"e_1_3_4_13_2","doi-asserted-by":"publisher","DOI":"10.1111\/1758-2229.12873"},{"key":"e_1_3_4_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01707-08"},{"key":"e_1_3_4_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jgar.2018.09.014"},{"key":"e_1_3_4_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0123-9392(10)70088-6"},{"key":"e_1_3_4_17_2","doi-asserted-by":"publisher","DOI":"10.1638\/1042-7260-44.1.173"},{"key":"e_1_3_4_18_2","doi-asserted-by":"publisher","DOI":"10.3390\/microorganisms9040834"},{"key":"e_1_3_4_19_2","unstructured":"CLSI. 2020. Performance Standards for Antimicrobial Susceptibility Testing; document M100. 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Call of the wild: antibiotic resistance genes in natural environments. Nat. Rev. 8:251\u2013259.","journal-title":"Nat. Rev"},{"key":"e_1_3_4_33_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1758-2229.2010.00142.x"},{"key":"e_1_3_4_34_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01167-19"},{"key":"e_1_3_4_35_2","doi-asserted-by":"publisher","DOI":"10.1111\/tbed.13686"},{"key":"e_1_3_4_36_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.66.10.4555-4558.2000"},{"key":"e_1_3_4_37_2","doi-asserted-by":"publisher","DOI":"10.1111\/1758-2229.12019"},{"key":"e_1_3_4_38_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00253-020-10670-4"},{"key":"e_1_3_4_39_2","first-page":"1804","volume-title":"Transbound. Emerg. Dis","author":"de Carvalho M.P.N.","year":"2020","unstructured":"de Carvalho M.P.N., , Fernandes M.R., , Sellera F.P., et al. 2020. International clones of extended-spectrum-\u03b2-lactamase (CTX-M)-producing Escherichia coli in peri-urban wild animals, Brazil. 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