{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T18:47:24Z","timestamp":1792003644073,"version":"4.4.0"},"reference-count":38,"publisher":"SAGE Publications","issue":"12","license":[{"start":{"date-parts":[[2021,12,1]],"date-time":"2021-12-01T00:00:00Z","timestamp":1638316800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Microbial Drug Resistance"],"published-print":{"date-parts":[[2021,12,1]]},"abstract":"<jats:p>\n                    Considering the concept of \u201cOne Health,\u201d the aim of this study was to determine susceptibility profiles of\n                    <jats:italic toggle=\"yes\">Escherichia coli<\/jats:italic>\n                    in piglets' intestinal microbiota from different farms in Portugal. Beyond antimicrobial susceptibility, the occurrence of multiple antibiotic resistance and detection of phenotypic\/genotypic extended-spectrum beta-lactamases (ESBLs) and plasmid mediated AmpC beta-lactamases (pAmpC) were done. From 10 different pig farms, 340\n                    <jats:italic toggle=\"yes\">E. coli<\/jats:italic>\n                    isolates were obtained from 75 feces samples. Susceptibility to amoxicillin-clavulanic acid (AMC), piperacillin (PIP), cefoxitin (FOX), ceftazidime (CAZ), cefepime (FEP), aztreonam (AZT), imipenem (IP), amikacin (AK), ciprofloxacin (CIP), and trimethoprim-sulfamethoxazole (SXT) was determined. Five-gene panel for amplification of\n                    <jats:italic toggle=\"yes\">bla<\/jats:italic>\n                    genes was used for ESBL (TEM, SHV, CTX-M) and pAmpC (CMY-2, ACC). Among\n                    <jats:italic toggle=\"yes\">E. coli<\/jats:italic>\n                    isolates, 209 were distributed in three resistance profiles: 57.7% MDR, 3.5% extensively drug-resistance (XDR) (resistant to CIP, SXT, and beta-lactams, except IP, with variability to AK) and 0.3% pandrug-resistance (PDR) (resistant to all antibiotics used). pAmpC and\/or ESBLs genes were presented in 65% of the isolates. Presence of different associations of\n                    <jats:italic toggle=\"yes\">bla<\/jats:italic>\n                    genes in the same isolate was the most observed (31%), and the most common were an ESBL (TEM) and a pAmpC (CMY-2). Presence of three or four\n                    <jats:italic toggle=\"yes\">bla<\/jats:italic>\n                    genes in various associations were detected. These isolates were very resistant, especially those with four genes, which were resistant to beta-lactams (except IP), CIP, and SXT. This study showed a surprisingly high rate of MDR\n                    <jats:italic toggle=\"yes\">E. coli<\/jats:italic>\n                    isolated in Portuguese piglets, with enzymes that impair activity of the most used antibiotics in human therapeutic.\n                  <\/jats:p>","DOI":"10.1089\/mdr.2020.0387","type":"journal-article","created":{"date-parts":[[2021,6,30]],"date-time":"2021-06-30T15:13:41Z","timestamp":1625066021000},"page":"1742-1749","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":8,"title":["Plasmid-Encoded AmpC and Extended-Spectrum Beta-Lactamases in Multidrug-Resistant\n                    <i>Escherichia coli<\/i>\n                    Isolated from Piglets in Portugal"],"prefix":"10.1177","volume":"27","author":[{"given":"Olga","family":"Cardoso","sequence":"first","affiliation":[{"name":"Univ Coimbra, CIEPQPF, FFUC, Coimbra, Portugal."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sofia","family":"Os\u00f3rio","sequence":"additional","affiliation":[{"name":"Augusto Virg\u00edlio Sousa &amp; Filho, Lda, Portugal."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fernando","family":"Ramos","sequence":"additional","affiliation":[{"name":"Univ Coimbra, FFUC, Coimbra, Portugal."},{"name":"Univ Porto, REQUIMTE\/LAQV, Porto, Portugal."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Manuel","family":"Donato","sequence":"additional","affiliation":[{"name":"Univ Coimbra, CIMAGO, Coimbra, Portugal."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2021,12,6]]},"reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S2542-5196(17)30141-9"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1503141112"},{"key":"e_1_3_3_4_2","unstructured":"World Health Organization. One Health. 2017. Available at www.who.int\/features\/qa\/one-health\/en accessed October30 2020."},{"key":"e_1_3_3_5_2","unstructured":"INE. 2017. Balan\u00e7a Alimentar Portuguesa 2012\u20132016. Available at www.ine.pt accessed October30 2020."},{"key":"e_1_3_3_6_2","unstructured":"https:\/\/www.racius.com\/suinicultura accessed March15 2021."},{"key":"e_1_3_3_7_2","unstructured":"European Parliament and the Council of the European Union. 2003. Regulation (EC) No 1831\/2003. Off. J. Eur. Union L 268\/29-42."},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.3390\/tropicalmed4010022"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.2175\/106143017X15023776270179"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/S2542-5196(19)30130-5"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1089\/mdr.2018.0184"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1080\/1040841X.2020.1715339"},{"key":"e_1_3_3_13_2","first-page":"278","article-title":"The European Union summary report on antimicrobial resistance in zoonotic and indicator bacteria from humans, animals and food in 2017","volume":"17","author":"EFSA (European Food Safety Authority) and ECDC (European Centre for Disease Prevention and Control)","year":"2019","unstructured":"EFSA (European Food Safety Authority) and ECDC (European Centre for Disease Prevention and Control). 2019. The European Union summary report on antimicrobial resistance in zoonotic and indicator bacteria from humans, animals and food in 2017. EFSA J. 17:5598, 278 pp.","journal-title":"EFSA J"},{"key":"e_1_3_3_14_2","first-page":"166","article-title":"The European Union Summary Report on Antimicrobial Resistance in zoonotic and indicator bacteria from humans, animals and food in 2017\/2018","volume":"18","author":"EFSA (European Food Safety Authority) and ECDC (European Centre for Disease Prevention and Control),","year":"2020","unstructured":"EFSA (European Food Safety Authority) and ECDC (European Centre for Disease Prevention and Control),. 2020. The European Union Summary Report on Antimicrobial Resistance in zoonotic and indicator bacteria from humans, animals and food in 2017\/2018. EFSA J. 18:6007, 166 pp.","journal-title":"EFSA J"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01076-18"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkx397"},{"key":"e_1_3_3_17_2","unstructured":"The European Committee on Antimicrobial Susceptibility Testing. 2019. Breakpoint tables for interpretation of MICs and zone diameters. Version 9.0. Available at www.eucast.org accessed October30 2020."},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-0691.2011.03570.x"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00219-10"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00622-08"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00576-07"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01943-07"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.1186\/s13756-019-0614-3"},{"key":"e_1_3_3_24_2","unstructured":"and WHO FAO. 2019. Joint FAO\/WHO Expert Meeting in collaboration with OIE on Foodborne Antimicrobial Resistance: Role of the Environment Crops and Biocides\u2014Meeting report. Microbiological Risk Assessment Series no. 34. Rome."},{"key":"e_1_3_3_25_2","doi-asserted-by":"publisher","DOI":"10.3390\/antibiotics9100678"},{"key":"e_1_3_3_26_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2672.2009.04570.x"},{"key":"e_1_3_3_27_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0157049"},{"key":"e_1_3_3_28_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0095623"},{"key":"e_1_3_3_29_2","volume-title":"et al","author":"Liu X.","year":"1756","unstructured":"Liu X., , Liu H., , Wang L., et al. 2018. Molecular characterization of extended-spectrum beta-lactamase-producing multidrug resistant, Escherichia coli, from swine in Northwest China. Front. 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Drug Resist. 25:1250\u20131256."},{"key":"e_1_3_3_37_2","doi-asserted-by":"publisher","DOI":"10.1111\/jfs.12282"},{"key":"e_1_3_3_38_2","doi-asserted-by":"publisher","DOI":"10.1111\/jam.14211"},{"key":"e_1_3_3_39_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0174094"}],"container-title":["Microbial Drug Resistance"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/full-xml\/10.1089\/mdr.2020.0387","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1089\/mdr.2020.0387","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1089\/mdr.2020.0387","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T20:38:35Z","timestamp":1777667915000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1089\/mdr.2020.0387"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,12,1]]},"references-count":38,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2021,12,1]]}},"alternative-id":["10.1089\/mdr.2020.0387"],"URL":"https:\/\/doi.org\/10.1089\/mdr.2020.0387","relation":{},"ISSN":["1076-6294","1931-8448"],"issn-type":[{"value":"1076-6294","type":"print"},{"value":"1931-8448","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,12,1]]}}}