{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T19:31:58Z","timestamp":1777923118109,"version":"3.51.4"},"reference-count":40,"publisher":"SAGE Publications","issue":"11","license":[{"start":{"date-parts":[[2021,11,1]],"date-time":"2021-11-01T00:00:00Z","timestamp":1635724800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Microbial Drug Resistance"],"published-print":{"date-parts":[[2021,11,1]]},"abstract":"<jats:p>\n                    The\n                    <jats:italic toggle=\"yes\">bla<\/jats:italic>\n                    genes identification present in 94 phenotypically resistant\n                    <jats:italic toggle=\"yes\">Escherichia coli<\/jats:italic>\n                    isolated from feces or intestinal contents of young calves with diarrhea or enteritis in Belgium was performed by microarrays (MA) and whole genome sequencing (WGS). According to their resistance phenotypes to 8 \u03b2-lactams at the disk diffusion assay these 94\n                    <jats:italic toggle=\"yes\">E. coli<\/jats:italic>\n                    produced a narrow-spectrum-\u03b2-lactamase (NSBL), an extended-spectrum-\u03b2-lactamase (ESBL) or a cephalosporinase (AmpC). All ESBL-encoding genes identified by MA and WGS belonged to the\n                    <jats:italic toggle=\"yes\">\n                      bla\n                      <jats:sub>CTX-M<\/jats:sub>\n                    <\/jats:italic>\n                    family, with a majority to the\n                    <jats:italic toggle=\"yes\">\n                      bla\n                      <jats:sub>CTX-M-1<\/jats:sub>\n                    <\/jats:italic>\n                    subfamily. Two different genes encoding an AmpC,\n                    <jats:italic toggle=\"yes\">\n                      bla\n                      <jats:sub>CMY-2<\/jats:sub>\n                    <\/jats:italic>\n                    , and\n                    <jats:italic toggle=\"yes\">\n                      bla\n                      <jats:sub>DHA-1<\/jats:sub>\n                    <\/jats:italic>\n                    were detected in isolates with an AmpC phenotype. The\n                    <jats:italic toggle=\"yes\">\n                      bla\n                      <jats:sub>TEM-1<\/jats:sub>\n                    <\/jats:italic>\n                    and the\n                    <jats:italic toggle=\"yes\">\n                      bla\n                      <jats:sub>OXA-1<\/jats:sub>\n                    <\/jats:italic>\n                    were detected alone in isolates with a NSBL phenotype or in combination with ESBL-\/AmpC-encoding\n                    <jats:italic toggle=\"yes\">bla<\/jats:italic>\n                    genes. Furthermore, the WGS identified mutations in the\n                    <jats:italic toggle=\"yes\">ampC<\/jats:italic>\n                    gene promoter at nucleotides -42 (C&gt;T) and\/or -18 (G&gt;A) that could not be identified by MA, in several isolates with an AmpC-like resistance phenotype. No carbapenemase-encoding gene was detected. To our knowledge this is the first survey on the identification of\n                    <jats:italic toggle=\"yes\">bla<\/jats:italic>\n                    genes in\n                    <jats:italic toggle=\"yes\">E. coli<\/jats:italic>\n                    isolated from young diarrheic or septicemic calves in Belgium.\n                  <\/jats:p>","DOI":"10.1089\/mdr.2020.0472","type":"journal-article","created":{"date-parts":[[2021,4,29]],"date-time":"2021-04-29T11:02:14Z","timestamp":1619694134000},"page":"1578-1584","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":8,"title":["Identification of \u03b2-Lactamase-Encoding (\n                    <i>bla<\/i>\n                    ) Genes in Phenotypically \u03b2-Lactam-Resistant\n                    <i>Escherichia coli<\/i>\n                    Isolated from Young Calves in Belgium"],"prefix":"10.1177","volume":"27","author":[{"given":"Virginie","family":"Gu\u00e9rin","sequence":"first","affiliation":[{"name":"Bacteriology, Department of Infectious and Parasitic Diseases, FARAH and Faculty of Veterinary Medicine, ULi\u00e8ge, Belgium."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Damien","family":"Thiry","sequence":"additional","affiliation":[{"name":"Bacteriology, Department of Infectious and Parasitic Diseases, FARAH and Faculty of Veterinary Medicine, ULi\u00e8ge, Belgium."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pierrick","family":"Lucas","sequence":"additional","affiliation":[{"name":"Anses Sequencing Platform, ANSES, Ploufragan-Plouzan\u00e9-Niort Laboratory, Ploufragan, France."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yannick","family":"Blanchard","sequence":"additional","affiliation":[{"name":"Anses Sequencing Platform, ANSES, Ploufragan-Plouzan\u00e9-Niort Laboratory, Ploufragan, France."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fr\u00e9d\u00e9ric","family":"Cawez","sequence":"additional","affiliation":[{"name":"Biological Macromolecules, Department of Life Sciences, Center for Protein Engineering (CIP), ULi\u00e8ge, Belgium."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paola Sandra","family":"Mercuri","sequence":"additional","affiliation":[{"name":"Biological Macromolecules, Department of Life Sciences, Center for Protein Engineering (CIP), ULi\u00e8ge, Belgium."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Moreno","family":"Galleni","sequence":"additional","affiliation":[{"name":"Biological Macromolecules, Department of Life Sciences, Center for Protein Engineering (CIP), ULi\u00e8ge, Belgium."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marc","family":"Saulmont","sequence":"additional","affiliation":[{"name":"Regional Animal Health and Identification Association (ARSIA), Ciney, Belgium."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jacques","family":"Mainil","sequence":"additional","affiliation":[{"name":"Bacteriology, Department of Infectious and Parasitic Diseases, FARAH and Faculty of Veterinary Medicine, ULi\u00e8ge, Belgium."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2021,11,11]]},"reference":[{"key":"e_1_3_5_2_2","unstructured":"Organisation for Economic Co-operation and Development (OECD). 2018. 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