{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T01:36:27Z","timestamp":1790732187817,"version":"4.1.0"},"reference-count":34,"publisher":"SAGE Publications","issue":"10","license":[{"start":{"date-parts":[[2021,10,1]],"date-time":"2021-10-01T00:00:00Z","timestamp":1633046400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Microbial Drug Resistance"],"published-print":{"date-parts":[[2021,10]]},"abstract":"<jats:p>\n                    In a large-scale dairy farm, it is important to take countermeasure of prevention against mastitis of dairy cows, and it is especially important to establish hygiene and risk management to prevent the emergence and spread of antibiotic-resistant bacteria. In this study, we have performed bacteriological testing of clinical and subclinical mastitis and investigation of antimicrobial resistance bacteria in a large-scale farm for 1 year. The bacteria isolated most frequently from 1,549 samples of 952 cow, including cows with recurring mastitis were\n                    <jats:italic toggle=\"yes\">Staphylococcus<\/jats:italic>\n                    non-\n                    <jats:italic toggle=\"yes\">aureus<\/jats:italic>\n                    (SNA) (27.6%), followed by\n                    <jats:italic toggle=\"yes\">Escherichia coli<\/jats:italic>\n                    (18.9%),\n                    <jats:italic toggle=\"yes\">Klebsiella pneumoniae<\/jats:italic>\n                    (12.3%). Although\n                    <jats:italic toggle=\"yes\">Staphylococcus aureus<\/jats:italic>\n                    was isolated at 7.7% from milk sample, no methicillin-resistant\n                    <jats:italic toggle=\"yes\">S. aureus<\/jats:italic>\n                    was found. The incidence of extended-spectrum \u03b2-lactamase (ESBL)-producing\n                    <jats:italic toggle=\"yes\">E. coli<\/jats:italic>\n                    was 1.4% and ESBL-producing\n                    <jats:italic toggle=\"yes\">K. pneumoniae<\/jats:italic>\n                    was 1.4% of all samples, even though third- and fourth-generation cephalosporins were not used for antimicrobial treatment of mastitis in this farm. Although these genotypes of ESBL-producing\n                    <jats:italic toggle=\"yes\">E. coli<\/jats:italic>\n                    and\n                    <jats:italic toggle=\"yes\">K. pneumoniae<\/jats:italic>\n                    were mainly composed of CTX-M-15 and TEM-1 and CTX-M-2 and TEM-116, respectively, there was no spread and persist of predominant clonal type. Appropriate farm management, such as segregation and culling of infected animals and monitors of trends in antimicrobial resistance among mastitis pathogens, may have contributed these results.\n                  <\/jats:p>","DOI":"10.1089\/mdr.2020.0481","type":"journal-article","created":{"date-parts":[[2021,4,26]],"date-time":"2021-04-26T12:32:33Z","timestamp":1619440353000},"page":"1450-1454","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":19,"title":["A 1-Year Investigation of Extended-Spectrum Beta-Lactamase-Producing\n                    <i>Escherichia coli<\/i>\n                    and\n                    <i>Klebsiella pneumoniae<\/i>\n                    Isolated from Bovine Mastitis at a Large-Scale Dairy Farm in Japan"],"prefix":"10.1177","volume":"27","author":[{"given":"Takako","family":"Taniguchi","sequence":"first","affiliation":[{"name":"Division of Research &amp; Inspection for Infectious Diseases, Center for Animal Disease Control, University of Miyazaki, Miyazaki, Japan."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Khin Maung","family":"Latt","sequence":"additional","affiliation":[{"name":"Laboratory of Veterinary Public Health, Department of Veterinary Science, University of Miyazaki, Miyazaki, Japan."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elpita","family":"Tarigan","sequence":"additional","affiliation":[{"name":"Division of Research &amp; Inspection for Infectious Diseases, Center for Animal Disease Control, University of Miyazaki, Miyazaki, Japan."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fusae","family":"Yano","sequence":"additional","affiliation":[{"name":"Division of Research &amp; Inspection for Infectious Diseases, Center for Animal Disease Control, University of Miyazaki, Miyazaki, Japan."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiroyuki","family":"Sato","sequence":"additional","affiliation":[{"name":"Laboratory of Veterinary Clinical Radiology, Department of Veterinary Medical Science, Faculty of Agriculture, University of Miyazaki, Miyazaki, Japan."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tomoya","family":"Minamino","sequence":"additional","affiliation":[{"name":"Veterinary Clinic, Honkawa Ranch, Hita, Japan."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Naoaki","family":"Misawa","sequence":"additional","affiliation":[{"name":"Division of Research &amp; Inspection for Infectious Diseases, Center for Animal Disease Control, University of Miyazaki, Miyazaki, Japan."},{"name":"Laboratory of Veterinary Public Health, Department of Veterinary Science, University of Miyazaki, Miyazaki, Japan."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2021,10,14]]},"reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1016\/S1090-0233(05)80007-3","article-title":"The costs of production diseases in dairy herds in England","volume":"154","author":"Kossaibati M.A.","year":"1997","unstructured":"Kossaibati M.A., , and Esslemont R.J.. 1997. The costs of production diseases in dairy herds in England. Vet. J. 154:41\u201351.","journal-title":"Vet. J"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2012.10.015"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00920-13"},{"key":"e_1_3_3_5_2","first-page":"373","volume":"16","author":"Holmes M.A.","year":"2011","unstructured":"Holmes M.A., , and Zadoks R.N.. 2011. Methicillin resistant S. aureus in human and bovine mastitis. J. Mammary Gland Biol. Neoplasia, 16:373\u2013382.","journal-title":"Methicillin resistant S. aureus in human and bovine mastitis. J. Mammary Gland Biol. 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