{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T19:31:34Z","timestamp":1777923094381,"version":"3.51.4"},"reference-count":5,"publisher":"SAGE Publications","issue":"8","license":[{"start":{"date-parts":[[2021,8,1]],"date-time":"2021-08-01T00:00:00Z","timestamp":1627776000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Microbial Drug Resistance"],"published-print":{"date-parts":[[2021,8]]},"abstract":"\n In this study, a rare strain of\n Escherichia coli<\/jats:italic>\n co-harboring\n bla<\/jats:italic>\n CTX-M-32<\/jats:sub>\n and\n bla<\/jats:italic>\n CTX-M-2<\/jats:sub>\n genes was characterized by whole genome sequencing.\n Escherichia coli<\/jats:italic>\n strain was recovered from a bovine cecal sample collected at slaughter. The strain was resistant to third and fourth generation cephalosporins, fluoroquinolones, aminoglycosides, tetracycline and sulfamethoxazole, which was in concordance with the genotype:\n bla<\/jats:italic>\n CTX-M-32<\/jats:sub>\n and\n bla<\/jats:italic>\n CTX-M-2<\/jats:sub>\n ,\n aac(3)<\/jats:italic>\n -Via and\n tet(A)<\/jats:italic>\n and\n sul1<\/jats:italic>\n genes, respectively. The\n E. coli<\/jats:italic>\n strain was characterized as pathogenic, and belongs to the ST226 (CC226), serotype O141ab\/ac:H5, phylogenetic group A and fumC27\/fimH41 types. No virulence genes were found in the genome. According to the plasmid analysis, IncFIB, IncHI2 and p0111 were present. The analysis of the contigs revealed that the\n bla<\/jats:italic>\n CTX-M-32<\/jats:sub>\n was located in the chromosome, upstream IS\n 5<\/jats:italic>\n -like\/IS\n Kpn26<\/jats:italic>\n and IS\n 1380<\/jats:italic>\n -like\/IS\n Ec9<\/jats:italic>\n sequences, whereas\n bla<\/jats:italic>\n CTX-M-2<\/jats:sub>\n was in the p0111 plasmid, within an integron class 1, and flanked by\n Orf3<\/jats:italic>\n \/qacE\u03941 and IS\n 91<\/jats:italic>\n \/IS\n CR1<\/jats:italic>\n . To our knowledge, this is the first report of a multidrug resistant\n Escherichia coli<\/jats:italic>\n strain co-harboring two CTX-M enzymes in a healthy bovine from Portugal. This finding allows a better understanding on the dissemination of these resistance mechanism among bacteria of animal origin.\n <\/jats:p>","DOI":"10.1089\/mdr.2020.0520","type":"journal-article","created":{"date-parts":[[2021,2,17]],"date-time":"2021-02-17T13:52:43Z","timestamp":1613569963000},"page":"1155-1157","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":7,"title":["Occurrence of a Rare Multidrug Resistant\n Escherichia coli<\/i>\n Coharboring\n bla<\/i>\n CTX-M-32<\/sub>\n and\n bla<\/i>\n CTX-M-2<\/sub>\n Genes in a Bovine"],"prefix":"10.1177","volume":"27","author":[{"given":"C\u00e9lia","family":"Le\u00e3o","sequence":"first","affiliation":[{"name":"Laboratory of Bacteriology and Mycology, National Institute of Agrarian and Veterinary Research (INIAV, IP), Oeiras, Portugal."},{"name":"Laboratory of Antibiotic Resistance, Environment and Development, MED\u2014Mediterranean Institute for Agriculture, \u00c9vora, Portugal."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lurdes","family":"Clemente","sequence":"additional","affiliation":[{"name":"Laboratory of Bacteriology and Mycology, National Institute of Agrarian and Veterinary Research (INIAV, IP), Oeiras, Portugal."},{"name":"Faculty of Veterinary Science, CIISA\u2014Centre for Interdisciplinary Research in Animal Health, Lisbon, Portugal."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vanessa","family":"Guerra","sequence":"additional","affiliation":[{"name":"Laboratory of Bacteriology and Mycology, National Institute of Agrarian and Veterinary Research (INIAV, IP), Oeiras, Portugal."},{"name":"Faculty of Science, University of Lisbon, Lisbon, Portugal."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ana","family":"Botelho","sequence":"additional","affiliation":[{"name":"Laboratory of Bacteriology and Mycology, National Institute of Agrarian and Veterinary Research (INIAV, IP), Oeiras, Portugal."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ana","family":"Amaro","sequence":"additional","affiliation":[{"name":"Laboratory of Bacteriology and Mycology, National Institute of Agrarian and Veterinary Research (INIAV, IP), Oeiras, Portugal."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2021,8,12]]},"reference":[{"key":"e_1_3_5_2_2","doi-asserted-by":"publisher","DOI":"10.3109\/1040841X.2012.691460"},{"key":"e_1_3_5_3_2","first-page":"pp","article-title":"Extended-spectrum-\u03b2-lactamase and carbapenemase-producing Enterobacteriaceae in food-producing animals in Europe","author":"Silva N.","year":"2019","unstructured":"Silva N., , Carvalho I., , Currie C., , Sousa M., , Igrejas G., , and Poeta P.. 2019. Extended-spectrum-\u03b2-lactamase and carbapenemase-producing Enterobacteriaceae in food-producing animals in Europe. In: J.-L. Capelo-Mart\u00ednez and G. Igrejas (ed.), Antibiotic Drug Resistance. John Wiley & Sons, Inc, New, Jersey, pp. 261\u2013273.","journal-title":"In: J.-L. Capelo-Mart\u00ednez and G. Igrejas (ed.), Antibiotic Drug Resistance. John Wiley & Sons, Inc, New"},{"key":"e_1_3_5_4_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2012.00110"},{"key":"e_1_3_5_5_2","doi-asserted-by":"crossref","first-page":"2308","DOI":"10.1128\/AAC.48.6.2308-2313.2004","article-title":"High-level resistance to ceftazidime conferred by a novel enzyme, CTX-M-32, derived from CTX-M-1 through a single Asp240-Gly substitution","volume":"48","author":"Cartelle M.","year":"2004","unstructured":"Cartelle M., , del Mar Tomas M., , Molina F., , Moure R., , Villanueva R., , and Bou G.. 2004. High-level resistance to ceftazidime conferred by a novel enzyme, CTX-M-32, derived from CTX-M-1 through a single Asp240-Gly substitution. Antimicrob. Agents Chemother. 48:2308\u20132313.","journal-title":"Antimicrob. Agents Chemother"},{"key":"e_1_3_5_6_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkp498"}],"container-title":["Microbial Drug Resistance"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/full-xml\/10.1089\/mdr.2020.0520","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1089\/mdr.2020.0520","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1089\/mdr.2020.0520","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T20:39:03Z","timestamp":1777667943000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/full\/10.1089\/mdr.2020.0520"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,8]]},"references-count":5,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2021,8]]}},"alternative-id":["10.1089\/mdr.2020.0520"],"URL":"https:\/\/doi.org\/10.1089\/mdr.2020.0520","relation":{},"ISSN":["1076-6294","1931-8448"],"issn-type":[{"value":"1076-6294","type":"print"},{"value":"1931-8448","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,8]]}}}