{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,27]],"date-time":"2026-10-27T19:07:56Z","timestamp":1793128076921,"version":"4.5.0"},"reference-count":43,"publisher":"SAGE Publications","issue":"3","license":[{"start":{"date-parts":[[2022,3,1]],"date-time":"2022-03-01T00:00:00Z","timestamp":1646092800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Microbial Drug Resistance"],"published-print":{"date-parts":[[2022,3,1]]},"abstract":"<jats:p>\n                    The detection rate of extended-spectrum \u03b2-lactamase (ESBL)-producing Enterobacterales, microorganisms associated with health care settings, has significantly increased worldwide. Moreover, their community incidence has increased in several countries. In this study, we investigated the prevalence and genetic diversity of ESBL-producing\n                    <jats:italic toggle=\"yes\">Escherichia coli<\/jats:italic>\n                    isolated from 547 nonduplicated stool specimens from healthy Japanese individuals, between 2015 and 2019.\n                    <jats:italic toggle=\"yes\">E. coli<\/jats:italic>\n                    were isolated on deoxycholate-hydrogen sulfide-lactose (DHL) agar and identified by MALDI-TOF MS, ESBL were screened through disk diffusion method (cefotaxime with or without clavulanate), and genetic detection and genotyping were performed by PCR and DNA sequencing. Clonal similarities between ESBL-producing and nonproducing isolates were assessed by multilocus sequence typing (MLST). The prevalence of ESBL-producing\n                    <jats:italic toggle=\"yes\">E. coli<\/jats:italic>\n                    was 9.7% (53\/547). These bacteria harbored CTX-M genes, from which CTX-M-9 (31\/53, 58.5%) and CTX-M-1 (13\/53, 24.5%) groups were the predominant. The MLST analysis revealed that ST131 genotype prevailed within ESBL-producing\n                    <jats:italic toggle=\"yes\">E. coli<\/jats:italic>\n                    (15\/53), whereas ST95 (10\/53) and ST73 (8\/53) prevailed among non-ESBL producers, with ST131 being present in only four isolates. Overall, a high prevalence rate of CTX-M-type ESBL-producing\n                    <jats:italic toggle=\"yes\">E. coli<\/jats:italic>\n                    was detected. CTX-M-9 group-producing ST131 predominated among healthy Japanese individuals, similar to that observed in hospital isolates. CTX-M-type ESBL may disseminate clonally among hospital patients and subsequently, within the community.\n                  <\/jats:p>","DOI":"10.1089\/mdr.2021.0062","type":"journal-article","created":{"date-parts":[[2022,1,6]],"date-time":"2022-01-06T14:36:11Z","timestamp":1641479771000},"page":"355-360","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":10,"title":["Predominance of CTX-M-9 Group Among ESBL-Producing\n                    <i>Escherichia coli<\/i>\n                    Isolated from Healthy Individuals in Japan"],"prefix":"10.1177","volume":"28","author":[{"given":"Takashi","family":"Masui","sequence":"first","affiliation":[{"name":"Department of Otolaryngology-Head and Neck Surgery, Nara Medical University, Kashihara, Japan."},{"name":"Department of Microbiology and Infectious Diseases, Nara Medical University, Kashihara, Japan."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2086-2591","authenticated-orcid":false,"given":"Ryuichi","family":"Nakano","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Infectious Diseases, Nara Medical University, Kashihara, Japan."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Akiyo","family":"Nakano","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Infectious Diseases, Nara Medical University, Kashihara, Japan."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kai","family":"Saito","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Infectious Diseases, Nara Medical University, Kashihara, Japan."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuki","family":"Suzuki","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Infectious Diseases, Nara Medical University, Kashihara, Japan."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Naoki","family":"Kakuta","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Infectious Diseases, Nara Medical University, Kashihara, Japan."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Saori","family":"Horiuchi","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Infectious Diseases, Nara Medical University, Kashihara, Japan."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kousuke","family":"Tsubaki","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Infectious Diseases, Nara Medical University, Kashihara, Japan."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tadashi","family":"Kitahara","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology-Head and Neck Surgery, Nara Medical University, Kashihara, Japan."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hisakazu","family":"Yano","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Infectious Diseases, Nara Medical University, Kashihara, Japan."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2022,3,4]]},"reference":[{"key":"e_1_3_4_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(08)70041-0"},{"key":"e_1_3_4_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhin.2009.02.021"},{"key":"e_1_3_4_4_2","first-page":"1897","volume-title":"Arch. 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