{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,4]],"date-time":"2026-09-04T23:02:24Z","timestamp":1788562944172,"version":"build-2803163510"},"reference-count":38,"publisher":"SAGE Publications","issue":"5","license":[{"start":{"date-parts":[[2022,5,1]],"date-time":"2022-05-01T00:00:00Z","timestamp":1651363200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Microbial Drug Resistance"],"published-print":{"date-parts":[[2022,5,1]]},"abstract":"<jats:p>\n                    We characterized plasmids carrying\n                    <jats:italic toggle=\"yes\">bla<\/jats:italic>\n                    <jats:sub>NDM-5<\/jats:sub>\n                    detected in\n                    <jats:italic toggle=\"yes\">Escherichia coli<\/jats:italic>\n                    isolated from the infection site and stool sample of a Japanese patient, with no international travel history, by whole-genome sequencing (WGS). WGS was performed using MiSeq and MinlON sequencer followed by hybrid\n                    <jats:italic toggle=\"yes\">de novo<\/jats:italic>\n                    assembly.\n                    <jats:italic toggle=\"yes\">bla<\/jats:italic>\n                    <jats:sub>NDM-5<\/jats:sub>\n                    was detected on IncX3 (\n                    <jats:italic toggle=\"yes\">bla<\/jats:italic>\n                    <jats:sub>NDM-5<\/jats:sub>\n                    \/IncX3) plasmids; pMTY18530-4_IncX3 in\n                    <jats:italic toggle=\"yes\">E. coli<\/jats:italic>\n                    TUM18530 isolated from a wound above the pubis; pMTY18780-5_IncX3 and pMTY18781-1_IncX3 in\n                    <jats:italic toggle=\"yes\">E. coli<\/jats:italic>\n                    TUM18780 and TUM18781, respectively, isolated from stool. These three plasmids resembled each other and pGSH8M-2-4, previously detected in\n                    <jats:italic toggle=\"yes\">E. coli<\/jats:italic>\n                    isolated from a Tokyo Bay water sample.\n                    <jats:italic toggle=\"yes\">E. coli<\/jats:italic>\n                    TUM18530 and TUM18780 belonged to sequence type (ST) 1011 and had only two single nucleotide polymorphisms on the core-genome, whereas TUM18781 belonged to ST2040. Three\n                    <jats:italic toggle=\"yes\">bla<\/jats:italic>\n                    <jats:sub>NDM-5<\/jats:sub>\n                    \/IncX3 plasmids (pMTY18530-4_IncX3, pMTY18780-5_IncX3, and pMTY18781-1_IncX3) exhibited conjugative transfer\n                    <jats:italic toggle=\"yes\">in vitro<\/jats:italic>\n                    at an average frequency of 1.71\u2009\u00d7\u200910\n                    <jats:sup>\u22123<\/jats:sup>\n                    per donor cell. The transconjugant was resistant to only \u03b2-lactams, including carbapenem, except aztreonam. Similarity of the\n                    <jats:italic toggle=\"yes\">bla<\/jats:italic>\n                    <jats:sub>NDM-5<\/jats:sub>\n                    \/IncX3 plasmids isolated from our patient compared with that isolated from the Tokyo bay water sample suggested that the plasmids may have already spread throughout the Japanese community. The\n                    <jats:italic toggle=\"yes\">bla<\/jats:italic>\n                    <jats:sub>NDM-5<\/jats:sub>\n                    \/IncX3 plasmid exhibited potential for easy transmission to different strains in the patient's intestine.\n                  <\/jats:p>","DOI":"10.1089\/mdr.2021.0197","type":"journal-article","created":{"date-parts":[[2022,5,11]],"date-time":"2022-05-11T14:58:20Z","timestamp":1652281100000},"page":"539-544","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":11,"title":["Whole-Genome Sequencing Analysis of\n                    <i>bla<\/i>\n                    <sub>NDM-5<\/sub>\n                    \/IncX3 Plasmid Estimated to Be Conjugative-Transferred in the Gut"],"prefix":"10.1177","volume":"28","author":[{"given":"Yukitaka","family":"Ito","sequence":"first","affiliation":[{"name":"Division of Clinical Microbiology Laboratory, Toho University Ohashi Medical Center, Meguro, Tokyo, Japan."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kotaro","family":"Aoki","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Infectious Diseases, Toho University School of Medicine, Ota, Tokyo, Japan."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoshikazu","family":"Ishii","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Infectious Diseases, Toho University School of Medicine, Ota, Tokyo, Japan."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haruo","family":"Nakayama","sequence":"additional","affiliation":[{"name":"Department of Infection and Prevention, Toho University Ohashi Medical Center, Meguro, Tokyo, Japan."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masanobu","family":"Otsuka","sequence":"additional","affiliation":[{"name":"Division of Clinical Microbiology Laboratory, Toho University Ohashi Medical Center, Meguro, Tokyo, Japan."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Naomi","family":"Kaneko","sequence":"additional","affiliation":[{"name":"Division of Clinical Microbiology Laboratory, Toho University Ohashi Medical Center, Meguro, Tokyo, Japan."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mieko","family":"Yoshida","sequence":"additional","affiliation":[{"name":"Division of Clinical Microbiology Laboratory, Toho University Ohashi Medical Center, Meguro, Tokyo, Japan."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kazuhiro","family":"Tateda","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Infectious Diseases, Toho University School of Medicine, Ota, Tokyo, Japan."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiroto","family":"Matsuse","sequence":"additional","affiliation":[{"name":"Department of Infection and Prevention, Toho University Ohashi Medical Center, Meguro, Tokyo, Japan."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2022,5,17]]},"reference":[{"key":"e_1_3_5_2_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/jiw282"},{"key":"e_1_3_5_3_2","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/ciq054"},{"key":"e_1_3_5_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.04265-14"},{"key":"e_1_3_5_5_2","doi-asserted-by":"publisher","DOI":"10.1099\/jmm.0.000793"},{"key":"e_1_3_5_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijantimicag.2017.01.003"},{"key":"e_1_3_5_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jgar.2019.07.001"},{"key":"e_1_3_5_8_2","doi-asserted-by":"publisher","DOI":"10.3390\/pathogens10080954"},{"key":"e_1_3_5_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijantimicag.2018.07.007"},{"key":"e_1_3_5_10_2","doi-asserted-by":"publisher","DOI":"10.1089\/mdr.2019.0382"},{"key":"e_1_3_5_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.envpol.2020.116370"},{"key":"e_1_3_5_12_2","doi-asserted-by":"publisher","DOI":"10.2147\/IDR.S215273"},{"key":"e_1_3_5_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.02238-20"},{"key":"e_1_3_5_14_2","first-page":"424","article-title":"Further spread of bla NDM-5 in Enterobacteriaceae via IncX3 plasmids in Shanghai, China","volume":"7","author":"Zhang F.","year":"2016","unstructured":"Zhang F., , Xie L., , Wang X., et al. 2016. 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