{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T19:31:52Z","timestamp":1777923112610,"version":"3.51.4"},"reference-count":40,"publisher":"SAGE Publications","issue":"5","license":[{"start":{"date-parts":[[2022,5,1]],"date-time":"2022-05-01T00:00:00Z","timestamp":1651363200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Microbial Drug Resistance"],"published-print":{"date-parts":[[2022,5,1]]},"abstract":"<jats:p>\n                    <jats:italic toggle=\"yes\">Streptococcus agalactiae<\/jats:italic>\n                    (Group B\n                    <jats:italic toggle=\"yes\">Streptococcus<\/jats:italic>\n                    , GBS) is a major agent of perinatal infections. Biofilms have been associated with GBS colonization and disease, as well as with infection persistence and recurrence. Although GBS remains susceptible to beta-lactams, it is still unknown how sessile cells respond to these antibiotics. Here, we evaluated the effect of different concentrations of penicillin (3\u201348\u2009mg\/L) on\n                    <jats:italic toggle=\"yes\">in vitro<\/jats:italic>\n                    biofilm formation by four GBS strains belonging to serotype Ia\/clonal complexes23 that were recovered from the oropharynx or urine of pregnant women and were previously characterized as strong biofilm producers. All four GBS strains were fully susceptible to penicillin (minimum inhibitory concentration\u2009=\u20090.023\u2009mg\/L), but penicillin was not able to fully prevent biofilm formation by these GBS strains. Biofilms formed in the presence of penicillin had reduced biomasses and thickness, but they were still classified as strong. Penicillin significantly reduced the density of live cells, but higher penicillin concentrations did not lead to improved prevention of biofilm formation. Biofilms formed in the presence of penicillin had no channels or long cocci chains observed in penicillin-free biofilms. Overall, results highlight the concerning possible impacts of biofilm formation in penicillin-based treatment and preventive strategies of GBS infections, even when the bacterial strain involved is fully antibiotic-susceptible.\n                  <\/jats:p>","DOI":"10.1089\/mdr.2021.0238","type":"journal-article","created":{"date-parts":[[2022,3,9]],"date-time":"2022-03-09T15:26:36Z","timestamp":1646839596000},"page":"517-524","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":2,"title":["Influence of Penicillin on Biofilm Formation by\n                    <i>Streptococcus agalactiae<\/i>\n                    Serotype Ia\/CC23"],"prefix":"10.1177","volume":"28","author":[{"given":"Danielle Cristina Santos Silva","family":"Alvim","sequence":"first","affiliation":[{"name":"Instituto de Microbiologia Paulo de G\u00f3es, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Laura Maria Andrade","family":"Oliveira","sequence":"additional","affiliation":[{"name":"Instituto de Microbiologia Paulo de G\u00f3es, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Leandro Corr\u00eaa","family":"Sim\u00f5es","sequence":"additional","affiliation":[{"name":"Instituto de Microbiologia Paulo de G\u00f3es, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Natalia Silva","family":"Costa","sequence":"additional","affiliation":[{"name":"Instituto de Microbiologia Paulo de G\u00f3es, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sergio Eduardo Longo","family":"Fracalanzza","sequence":"additional","affiliation":[{"name":"Instituto de Microbiologia Paulo de G\u00f3es, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lucia Martins","family":"Teixeira","sequence":"additional","affiliation":[{"name":"Instituto de Microbiologia Paulo de G\u00f3es, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rosana Barreto Rocha","family":"Ferreira","sequence":"additional","affiliation":[{"name":"Instituto de Microbiologia Paulo de G\u00f3es, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7878-9377","authenticated-orcid":false,"given":"Tatiana Castro Abreu","family":"Pinto","sequence":"additional","affiliation":[{"name":"Instituto de Microbiologia Paulo de G\u00f3es, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2022,5,17]]},"reference":[{"key":"e_1_3_5_2_2","first-page":"S100","article-title":"GBS Maternal Colonization Investigator Group","volume":"65","author":"Russell N.J.","year":"2017","unstructured":"Russell N.J., , Seale A.C, , O'Driscoll M., et al. 2017. GBS Maternal Colonization Investigator Group. Maternal colonization with group B Streptococcus and Serotype distribution worldwide: systematic review and meta-analyses. Clin. Infect. Dis. 65:S100\u2013S111.","journal-title":"Maternal colonization with group B Streptococcus and Serotype distribution worldwide: systematic review and meta-analyses. Clin. Infect. 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