{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T19:33:17Z","timestamp":1777923197077,"version":"3.51.4"},"reference-count":46,"publisher":"SAGE Publications","issue":"3","license":[{"start":{"date-parts":[[2022,3,1]],"date-time":"2022-03-01T00:00:00Z","timestamp":1646092800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Microbial Drug Resistance"],"published-print":{"date-parts":[[2022,3,1]]},"abstract":"<jats:sec>\n                    <jats:title>Objective:<\/jats:title>\n                    <jats:p>\n                      This study aimed to reveal the prevalence and fitness of\n                      <jats:italic toggle=\"yes\">qnrS1<\/jats:italic>\n                      -carrying plasmids in hypervirulent\n                      <jats:italic toggle=\"yes\">Klebsiella pneumoniae<\/jats:italic>\n                      (hvKP) isolates.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Materials and Methods:<\/jats:title>\n                    <jats:p>\n                      Two hundred ninety-nine hvKP strains carrying qnrS1 were collected and screened for resistance genes using PCR and sequencing. The location of\n                      <jats:italic toggle=\"yes\">qnrS1<\/jats:italic>\n                      and\n                      <jats:italic toggle=\"yes\">rmpA2<\/jats:italic>\n                      was identified by Southern blotting. The transferability and fitness of\n                      <jats:italic toggle=\"yes\">qnrS1<\/jats:italic>\n                      -carrying plasmids were analyzed by conjugation experiments and plasmid stability assay.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Result:<\/jats:title>\n                    <jats:p>\n                      In 299 hvKP isolates, the most frequently detected capsular serotype was K64 (81.9%, 245\/299), followed by K1 (4.7%, 14\/299) and K2 (3.7%, 11\/299). All K64-hvKP were sequence type (ST) 11. The\n                      <jats:italic toggle=\"yes\">qnrS1<\/jats:italic>\n                      and\n                      <jats:italic toggle=\"yes\">rmpA2<\/jats:italic>\n                      gene mainly was located on the \u223c70\u2013210\u2009kb IncFrepB and \u223c170\u2013220\u2009kb IncFIB plasmid, respectively. QnrS1-carrying plasmids could be transferred into\n                      <jats:italic toggle=\"yes\">Escherichia coli<\/jats:italic>\n                      J53. However, the plasmid was transferred at a low rate of 13.4% (40\/299). The 40 donor isolates belong to 4 STs\u2014ST11, ST700, ST592, and ST86, and none contains the CRISPR-Cas loci. CRISPR-Cas loci were mainly found in ST23\n                      <jats:italic toggle=\"yes\">K. pneumoniae<\/jats:italic>\n                      . The relative fitness (RF) of\n                      <jats:italic toggle=\"yes\">qnrS1<\/jats:italic>\n                      -carrying plasmids in ST86 and ST11 (cotransfer with\n                      <jats:italic toggle=\"yes\">bla<\/jats:italic>\n                      <jats:sub>TEM-1<\/jats:sub>\n                      genes) was more than one and enhanced during cultivation, especially in ST86. However, the RF of\n                      <jats:italic toggle=\"yes\">qnrS1<\/jats:italic>\n                      -carrying plasmids in ST592 and ST700 showed a high fitness cost. Whole-genome sequencing showed that the\n                      <jats:italic toggle=\"yes\">qnrS1<\/jats:italic>\n                      -carrying plasmids in ST86 harbored more maintenance modules (SOS inhibitor protein\n                      <jats:italic toggle=\"yes\">psiB<\/jats:italic>\n                      ,\n                      <jats:italic toggle=\"yes\">parA<\/jats:italic>\n                      , and\n                      <jats:italic toggle=\"yes\">parB<\/jats:italic>\n                      partition systems) and insertion sequence (IS) elements (IS91, IS481-like, IS1380), indicating that the\n                      <jats:italic toggle=\"yes\">qnrS1<\/jats:italic>\n                      -carrying plasmid in ST86 is more stable than the other types of\n                      <jats:italic toggle=\"yes\">qnrS1<\/jats:italic>\n                      -carrying plasmids.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion:<\/jats:title>\n                    <jats:p>\n                      <jats:italic toggle=\"yes\">QnrS1<\/jats:italic>\n                      -carrying IncFrepB plasmids were highly prevalent and show polymorphism in hvKP strains. The\n                      <jats:italic toggle=\"yes\">qnrS1<\/jats:italic>\n                      -carrying IncFrepB plasmid in ST86 hvKP should be highlighted due to its remarkable adaptability advantages.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1089\/mdr.2021.0241","type":"journal-article","created":{"date-parts":[[2022,1,12]],"date-time":"2022-01-12T23:59:04Z","timestamp":1642031944000},"page":"361-369","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":3,"title":["High Prevalence and Fitness of IncFrepB Carrying\n                    <i>qnrS1<\/i>\n                    in Hypervirulent\n                    <i>Klebsiella pneumoniae<\/i>\n                    Isolates"],"prefix":"10.1177","volume":"28","author":[{"given":"Si","family":"Chen","sequence":"first","affiliation":[{"name":"Department of Bacteriology, The First Affiliated Hospital of Nanchang University, Nanchang, P.R. China."},{"name":"School of Public Health, Nanchang University, Nanchang, P.R. China."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lagen","family":"Wan","sequence":"additional","affiliation":[{"name":"Department of Bacteriology, The First Affiliated Hospital of Nanchang University, Nanchang, P.R. China."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dan-Dan","family":"Wei","sequence":"additional","affiliation":[{"name":"Department of Bacteriology, The First Affiliated Hospital of Nanchang University, Nanchang, P.R. China."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fanglin","family":"Du","sequence":"additional","affiliation":[{"name":"Department of Bacteriology, The First Affiliated Hospital of Nanchang University, Nanchang, P.R. China."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qi-Seng","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Bacteriology, The First Affiliated Hospital of Nanchang University, Nanchang, P.R. China."},{"name":"School of Public Health, Nanchang University, Nanchang, P.R. China."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yang","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Bacteriology, The First Affiliated Hospital of Nanchang University, Nanchang, P.R. China."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2022,3,4]]},"reference":[{"key":"e_1_3_5_2_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2017.01576"},{"key":"e_1_3_5_3_2","doi-asserted-by":"publisher","DOI":"10.2147\/IDR.S161075"},{"key":"e_1_3_5_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01127-16"},{"key":"e_1_3_5_5_2","doi-asserted-by":"publisher","DOI":"10.4161\/viru.22718"},{"key":"e_1_3_5_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijid.2015.10.013"},{"key":"e_1_3_5_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijid.2020.09.1485"},{"key":"e_1_3_5_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.02173-15"},{"key":"e_1_3_5_9_2","doi-asserted-by":"publisher","DOI":"10.1089\/mdr.2018.0173"},{"key":"e_1_3_5_10_2","doi-asserted-by":"publisher","DOI":"10.1017\/ice.2019.155"},{"key":"e_1_3_5_11_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0156690"},{"key":"e_1_3_5_12_2","doi-asserted-by":"publisher","DOI":"10.1186\/s13756-019-0552-0"},{"key":"e_1_3_5_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jgar.2017.10.023"},{"key":"e_1_3_5_14_2","doi-asserted-by":"publisher","DOI":"10.1007\/s11033-019-05220-9"},{"key":"e_1_3_5_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.meegid.2018.02.003"},{"key":"e_1_3_5_16_2","first-page":"1387","article-title":"Correlation of quinolone-resistance, qnr genes and integron carriage in multidrug-resistant community isolates of Klebsiella spp","volume":"22","author":"Malek Jamshidi M.R.","year":"2019","unstructured":"Malek Jamshidi M.R., , Zandi H., , and Eftekhar F.. 2019. 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