{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,3]],"date-time":"2026-08-03T22:55:58Z","timestamp":1785797758599,"version":"3.56.0"},"reference-count":78,"publisher":"SAGE Publications","issue":"4","license":[{"start":{"date-parts":[[2022,4,1]],"date-time":"2022-04-01T00:00:00Z","timestamp":1648771200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Microbial Drug Resistance"],"published-print":{"date-parts":[[2022,4,1]]},"abstract":"<jats:p>\n                    The emergence of vancomycin-resistant\n                    <jats:italic toggle=\"yes\">Enterococcus faecium<\/jats:italic>\n                    (Efm) harboring\n                    <jats:italic toggle=\"yes\">vanA<\/jats:italic>\n                    gene and multidrug-resistant determinants is a relevant public health concern. It is an opportunistic pathogen responsible for nosocomial infections widely distributed in the environment, including wastewater treatment plants (WWTPs). Our study addresses a genomic investigation of\n                    <jats:italic toggle=\"yes\">vanA<\/jats:italic>\n                    -carrying Efm from WWTPs in Brazil. Samples from five WWTPs supplied with sewage from different sources were evaluated. Here we present whole-genome sequencing of eight\n                    <jats:italic toggle=\"yes\">vanA<\/jats:italic>\n                    -Efm isolates performed on Illumina MiSeq platform. All these isolates presented multidrug-resistant profile, and five strains were from treated wastewater. Multiple antimicrobial resistance genes (ARGs) were found, such as\n                    <jats:italic toggle=\"yes\">aph(3\u2032)-IIIa<\/jats:italic>\n                    ,\n                    <jats:italic toggle=\"yes\">ant(6\u2032)-Ia<\/jats:italic>\n                    ,\n                    <jats:italic toggle=\"yes\">erm(B)<\/jats:italic>\n                    , and\n                    <jats:italic toggle=\"yes\">msrC<\/jats:italic>\n                    , some of them being allocated in plasmids. The virulence profile was predominantly constituted by\n                    <jats:italic toggle=\"yes\">efaAfm<\/jats:italic>\n                    and\n                    <jats:italic toggle=\"yes\">acm<\/jats:italic>\n                    genes and all isolates, except for one, were predicted as human pathogens. Multilocus sequence typing analysis revealed a new allele and five different STs, three previously described (ST32, ST168, and ST253) and two novel ones (ST1893 and ST1894). Six strains belonged to CC17, often associated with hospital outbreaks. As far as our knowledge, no genomic studies of\n                    <jats:italic toggle=\"yes\">vanA<\/jats:italic>\n                    -Efm recovered from WWTPs revealed isolates belonging to CC17 in Brazil. Therefore, our findings point to the environmental spread of Efm carrying multiple ARGs.\n                  <\/jats:p>","DOI":"10.1089\/mdr.2021.0254","type":"journal-article","created":{"date-parts":[[2022,2,16]],"date-time":"2022-02-16T18:07:20Z","timestamp":1645034840000},"page":"444-452","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":13,"title":["Genomic Analysis of Multidrug-Resistant\n                    <i>Enterococcus faecium<\/i>\n                    Harboring\n                    <i>vanA<\/i>\n                    Gene from Wastewater Treatment Plants"],"prefix":"10.1177","volume":"28","author":[{"given":"Beatriz Oliveira de","family":"Farias","sequence":"first","affiliation":[{"name":"Funda\u00e7\u00e3o Oswaldo Cruz, Instituto Nacional de Controle de Qualidade em Sa\u00fade\u2014Avenida Brasil, Rio de Janeiro, Rio de Janeiro, Brasil."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7520-4097","authenticated-orcid":false,"given":"Kayo","family":"Bianco","sequence":"additional","affiliation":[{"name":"Funda\u00e7\u00e3o Oswaldo Cruz, Instituto Nacional de Controle de Qualidade em Sa\u00fade\u2014Avenida Brasil, Rio de Janeiro, Rio de Janeiro, Brasil."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana Paula Alves","family":"Nascimento","sequence":"additional","affiliation":[{"name":"Funda\u00e7\u00e3o Oswaldo Cruz, Instituto Nacional de Controle de Qualidade em Sa\u00fade\u2014Avenida Brasil, Rio de Janeiro, Rio de Janeiro, Brasil."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andressa Silva","family":"Gon\u00e7alves de Brito","sequence":"additional","affiliation":[{"name":"Funda\u00e7\u00e3o Oswaldo Cruz, Instituto Nacional de Controle de Qualidade em Sa\u00fade\u2014Avenida Brasil, Rio de Janeiro, Rio de Janeiro, Brasil."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thais Costa","family":"Moreira","sequence":"additional","affiliation":[{"name":"Funda\u00e7\u00e3o Oswaldo Cruz, Instituto Nacional de Controle de Qualidade em Sa\u00fade\u2014Avenida Brasil, Rio de Janeiro, Rio de Janeiro, Brasil."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6320-712X","authenticated-orcid":false,"given":"Maysa Mandetta","family":"Clementino","sequence":"additional","affiliation":[{"name":"Funda\u00e7\u00e3o Oswaldo Cruz, Instituto Nacional de Controle de Qualidade em Sa\u00fade\u2014Avenida Brasil, Rio de Janeiro, Rio de Janeiro, Brasil."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2022,4,18]]},"reference":[{"key":"e_1_3_4_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jgar.2018.08.016"},{"key":"e_1_3_4_3_2","first-page":"137275","article-title":"Comparative genomics of vancomycin-resistant Enterococcus spp","volume":"719","author":"Ekwanzala M.D.","year":"2020","unstructured":"Ekwanzala M.D., , Dewar J.B, , Kamika I., , and Momba M.N.B. 2020. Comparative genomics of vancomycin-resistant Enterococcus spp. revealed common resistome determinants from hospital wastewater to aquatic environments. Sci. Total Environ. 719:137275.","journal-title":"revealed common resistome determinants from hospital wastewater to aquatic environments. Sci. Total Environ"},{"key":"e_1_3_4_4_2","doi-asserted-by":"publisher","DOI":"10.3390\/ijerph120404231"},{"key":"e_1_3_4_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ccc.2013.06.006"},{"key":"e_1_3_4_6_2","first-page":"237","article-title":"Clinical management of non-faecium non-faecalis vancomycin-resistant enterococci infection","volume":"24","author":"Monticelli J.","year":"2018","unstructured":"Monticelli J., , Knezevich A., , Luzzati R., , and Di Bella S.. 2018. Clinical management of non-faecium non-faecalis vancomycin-resistant enterococci infection. Focus on Enterococcus gallinarum and Enterococcus casseliflavus\/flavescens. J. Infect. 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