{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,6]],"date-time":"2026-05-06T01:54:14Z","timestamp":1778032454578,"version":"3.51.4"},"reference-count":21,"publisher":"SAGE Publications","issue":"4","license":[{"start":{"date-parts":[[2022,4,1]],"date-time":"2022-04-01T00:00:00Z","timestamp":1648771200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Microbial Drug Resistance"],"published-print":{"date-parts":[[2022,4,1]]},"abstract":"<jats:p>\n                    <jats:italic toggle=\"yes\">Pantoea<\/jats:italic>\n                    spp. are bacteria that are often detected in the environment and as symbionts of arthropods. They sporadically cause infections in humans and recently extended spectrum beta-lactamases (ESBL)- and carbapenemase-producing strains have started to emerge. In this study, we report the isolation and the complete genome sequence of a strain of\n                    <jats:italic toggle=\"yes\">Pantoea calida<\/jats:italic>\n                    encoding the colistin-resistance gene\n                    <jats:italic toggle=\"yes\">mcr-9<\/jats:italic>\n                    . The strain was isolated from a preterm newborn in a neonatal pathology ward. On clinical examination, his vital signs were normal and blood culture was negative. Rectal swab screening for ESBL-producing\n                    <jats:italic toggle=\"yes\">Enterobacterales<\/jats:italic>\n                    allowed to isolate the bacterium, and a complete genome was obtained using both short and long read sequencing. The\n                    <jats:italic toggle=\"yes\">mcr-9<\/jats:italic>\n                    gene was found to be encoded on a IncHI2 superplasmid, which confers resistance to six classes of antibiotics, including beta lactams (ESBL). Despite the presence of\n                    <jats:italic toggle=\"yes\">mcr-9<\/jats:italic>\n                    , the isolate retains susceptibility to colistin, which could be explained by the absence of compatible regulatory genes (\n                    <jats:italic toggle=\"yes\">qseBC)<\/jats:italic>\n                    from the genome. The presence of the resistance gene is undetectable with the routine clinical procedures, that is, phenotypic tests. This suggests that a silent spread might be ongoing in the ward. To our knowledge, this is the first description of an MDR\n                    <jats:italic toggle=\"yes\">P. calida<\/jats:italic>\n                    and of a\n                    <jats:italic toggle=\"yes\">Pantoea<\/jats:italic>\n                    spp. encoding any mobile colistin resistance gene.\n                  <\/jats:p>","DOI":"10.1089\/mdr.2021.0267","type":"journal-article","created":{"date-parts":[[2022,3,29]],"date-time":"2022-03-29T13:44:26Z","timestamp":1648561466000},"page":"408-412","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":12,"title":["Isolation of a Colistin-Susceptible MDR\n                    <i>Pantoea calida<\/i>\n                    Harboring the\n                    <i>mcr-9<\/i>\n                    Gene Suggests the Silent Spread of the Resistance Factor"],"prefix":"10.1177","volume":"28","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5847-6568","authenticated-orcid":false,"given":"Stefano","family":"Gaiarsa","sequence":"first","affiliation":[{"name":"U.O.C. Microbiologia e Virologia, Fondazione IRCCS Policlinico San Matteo, Pavia, Italy."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cristina","family":"Merla","sequence":"additional","affiliation":[{"name":"U.O.C. Microbiologia e Virologia, Fondazione IRCCS Policlinico San Matteo, Pavia, Italy."},{"name":"Scuola di Specializzazione in Microbiologia e Virologia, Universit\u00e0 degli Studi di Pavia, Pavia, Italy."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0969-6484","authenticated-orcid":false,"given":"Marta","family":"Corbella","sequence":"additional","affiliation":[{"name":"U.O.C. Microbiologia e Virologia, Fondazione IRCCS Policlinico San Matteo, Pavia, Italy."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bianca","family":"Mariani","sequence":"additional","affiliation":[{"name":"U.O.C. Microbiologia e Virologia, Fondazione IRCCS Policlinico San Matteo, Pavia, Italy."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michela","family":"Zatelli","sequence":"additional","affiliation":[{"name":"U.O.C. Microbiologia e Virologia, Fondazione IRCCS Policlinico San Matteo, Pavia, Italy."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Irene","family":"Sciabica","sequence":"additional","affiliation":[{"name":"U.O.C. Microbiologia e Virologia, Fondazione IRCCS Policlinico San Matteo, Pavia, Italy."},{"name":"Scuola di Specializzazione in Microbiologia e Virologia, Universit\u00e0 degli Studi di Pavia, Pavia, Italy."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michele","family":"Castelli","sequence":"additional","affiliation":[{"name":"Dipartimento di Biologia e Biotecnologie L. Spallanzani, Universit\u00e0 degli Studi di Pavia, Pavia, Italy."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aurora","family":"Piazza","sequence":"additional","affiliation":[{"name":"Dipartimento di Scienze Clinico-Chirurgiche, Diagnostiche e Pediatriche\u2014Universit\u00e0 degli Studi di Pavia, Pavia, Italy."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marco","family":"Zecca","sequence":"additional","affiliation":[{"name":"U.O.C. Neonatologia e Terapia intensiva neonatale, Fondazione IRCCS Policlinico San Matteo, Pavia, Italy."},{"name":"U.O.C. Ematologia ed Oncologia Pediatrica, Fondazione IRCCS Policlinico San Matteo, Pavia, Italy."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Davide","family":"Sassera","sequence":"additional","affiliation":[{"name":"Dipartimento di Biologia e Biotecnologie L. Spallanzani, Universit\u00e0 degli Studi di Pavia, Pavia, Italy."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Patrizia","family":"Cambieri","sequence":"additional","affiliation":[{"name":"U.O.C. Microbiologia e Virologia, Fondazione IRCCS Policlinico San Matteo, Pavia, Italy."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2022,4,18]]},"reference":[{"key":"e_1_3_6_2_2","doi-asserted-by":"publisher","DOI":"10.1111\/1462-2920.15169"},{"key":"e_1_3_6_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jiph.2017.07.020"},{"key":"e_1_3_6_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhin.2016.09.009"},{"key":"e_1_3_6_5_2","volume-title":"Pantoea agglomerans","author":"Hussein A.H.","unstructured":"Hussein A.H., , Hussein N.H, , Taha B.M, , Rasool K.H, , and Al-Kadmy I.. 2020. Distribution of Pantoea agglomerans. isolates and molecular detection of blaPER-1 ES\u03b2L gene: a statistical study. Microb. Pathog. 147:10, 4374."},{"key":"e_1_3_6_6_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmed.2017.00183"},{"key":"e_1_3_6_7_2","doi-asserted-by":"publisher","DOI":"10.1186\/1752-1947-8-195"},{"key":"e_1_3_6_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jiac.2017.01.001"},{"key":"e_1_3_6_9_2","first-page":"e00965","article-title":"mcr-9, an inducible gene encoding an acquired phosphoethanolamine transferase in Escherichia coli, and its origin","volume":"63","author":"Kieffer N.","year":"2019","unstructured":"Kieffer N., , Royer G., , Decousser J.W, et al. 2019. mcr-9, an inducible gene encoding an acquired phosphoethanolamine transferase in Escherichia coli, and its origin. Antimicrob. Agents Chemother. 63:e00965-19.","journal-title":"Antimicrob. Agents Chemother"},{"key":"e_1_3_6_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(15)00424-7"},{"key":"e_1_3_6_11_2","first-page":"e00853","volume":"10","author":"Carroll L.M.","year":"2019","unstructured":"Carroll L.M., , Gaballa A., , Guldimann C., , Sullivan G., , Henderson L.O, , and Wiedmann M.. 2019. Identification of novel mobilized colistin resistance gene in a multidrug-resistant, colistin-susceptible Salmonella enterica Serotype Typhimurium isolate. mBio. 10:e00853-19.","journal-title":"Identification of novel mobilized colistin resistance gene in a multidrug-resistant, colistin-susceptible Salmonella enterica Serotype Typhimurium isolate. mBio"},{"key":"e_1_3_6_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00573-20"},{"key":"e_1_3_6_13_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2020.00080"},{"key":"e_1_3_6_14_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pcbi.1005595"},{"key":"e_1_3_6_15_2","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btu153"},{"key":"e_1_3_6_16_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.02412-14"},{"key":"e_1_3_6_17_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.03617-13"},{"key":"e_1_3_6_18_2","doi-asserted-by":"publisher","DOI":"10.1186\/s13059-016-0997-x"},{"key":"e_1_3_6_19_2","doi-asserted-by":"publisher","DOI":"10.1101\/gr.2289704"},{"key":"e_1_3_6_20_2","doi-asserted-by":"publisher","DOI":"10.21105\/joss.01762"},{"key":"e_1_3_6_21_2","doi-asserted-by":"crossref","unstructured":"Macesic N. Blakeway L.V Stewart J.D et al. 2021. Silent spread of mobile colistin resistance gene mcr-9.1 on IncHI2 \u2018superplasmids' in clinical carbapenem-resistant Enterobacterales. Clin. Microbiol. Infect. 27:1856.e7\u20131856.e13.","DOI":"10.1016\/j.cmi.2021.04.020"},{"key":"e_1_3_6_22_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-020-65106-w"}],"container-title":["Microbial Drug Resistance"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/full-xml\/10.1089\/mdr.2021.0267","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1089\/mdr.2021.0267","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1089\/mdr.2021.0267","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T20:39:52Z","timestamp":1777667992000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1089\/mdr.2021.0267"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,4,1]]},"references-count":21,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2022,4,1]]}},"alternative-id":["10.1089\/mdr.2021.0267"],"URL":"https:\/\/doi.org\/10.1089\/mdr.2021.0267","relation":{},"ISSN":["1076-6294","1931-8448"],"issn-type":[{"value":"1076-6294","type":"print"},{"value":"1931-8448","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,4,1]]}}}