{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,13]],"date-time":"2026-07-13T15:28:03Z","timestamp":1783956483105,"version":"3.55.0"},"reference-count":30,"publisher":"SAGE Publications","issue":"6","license":[{"start":{"date-parts":[[2022,6,1]],"date-time":"2022-06-01T00:00:00Z","timestamp":1654041600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Microbial Drug Resistance"],"published-print":{"date-parts":[[2022,6,1]]},"abstract":"<jats:sec>\n                    <jats:title>Objectives:<\/jats:title>\n                    <jats:p>\n                      To characterize the antimicrobial resistance and virulence of pathogenic\n                      <jats:italic toggle=\"yes\">Escherichia coli<\/jats:italic>\n                      isolated from diseased captive giant pandas.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods:<\/jats:title>\n                    <jats:p>Antimicrobial susceptibility and minimum inhibitory concentration (MIC) were determined by the broth dilution method. Whole-genome sequencing was used to characterize the phylogeny, serotype, virulence, resistome, plasmids, and genetic structures of the cefotaxime (CTX)-M genes.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results:<\/jats:title>\n                    <jats:p>\n                      Four extended-spectrum beta-lactamase (ESBL)-producing\n                      <jats:italic toggle=\"yes\">E. coli<\/jats:italic>\n                      strains were identified and the MICs against 11 antibiotics\n                      <jats:italic toggle=\"yes\">in vitro<\/jats:italic>\n                      were determined. All ESBL-producing\n                      <jats:italic toggle=\"yes\">E. coli<\/jats:italic>\n                      strains were resistant to more than eight antibiotics and carried the\n                      <jats:italic toggle=\"yes\">bla<\/jats:italic>\n                      <jats:sub>CTX-M-55<\/jats:sub>\n                      or\n                      <jats:italic toggle=\"yes\">bla<\/jats:italic>\n                      <jats:sub>CTX-M-105<\/jats:sub>\n                      gene in different sizes of replicon-type plasmids (pAMSH1-IncHI2, 257\u2009kb; pAMPD2-IncFII, 89\u2009kb; pAMPD02-IncFIB, 129\u2009kb; and pAMSC4-IncN, 47\u2009kb). Distinct insertional sequences and transposases were identified up-\/downstream of\n                      <jats:italic toggle=\"yes\">bla<\/jats:italic>\n                      <jats:sub>CTX-Ms<\/jats:sub>\n                      , including IS\n                      <jats:italic toggle=\"yes\">26<\/jats:italic>\n                      , IS\n                      <jats:italic toggle=\"yes\">Ecp1<\/jats:italic>\n                      , IS\n                      <jats:italic toggle=\"yes\">Kpn72<\/jats:italic>\n                      , IS\n                      <jats:italic toggle=\"yes\">903B<\/jats:italic>\n                      , and Tn\n                      <jats:italic toggle=\"yes\">2<\/jats:italic>\n                      . These strains also possessed at least three virulence genes of pathogenic\n                      <jats:italic toggle=\"yes\">E. coli<\/jats:italic>\n                      and originated in four different evolutionary branches. One strain carried the complete locus of the enterocyte effacement pathogenicity island, but lacked the virulence genes\n                      <jats:italic toggle=\"yes\">stx<\/jats:italic>\n                      and\n                      <jats:italic toggle=\"yes\">bfpA<\/jats:italic>\n                      , indicating atypical enteropathogenic\n                      <jats:italic toggle=\"yes\">E. coli<\/jats:italic>\n                      , whereas the other strains were considered to be extraintestinal pathogenic\n                      <jats:italic toggle=\"yes\">E. coli<\/jats:italic>\n                      .\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions:<\/jats:title>\n                    <jats:p>\n                      The emergence of ESBL-producing pathogenic\n                      <jats:italic toggle=\"yes\">E. coli<\/jats:italic>\n                      strains from diseased captive giant pandas warrants greater attention. The findings of this study will help to prevent the spread of these strains among captive giant pandas as well as from wild animals to humans.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1089\/mdr.2021.0298","type":"journal-article","created":{"date-parts":[[2022,5,31]],"date-time":"2022-05-31T11:35:02Z","timestamp":1653996902000},"page":"750-757","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":9,"title":["Molecular Characteristics of Extended-Spectrum Beta-Lactamase-Producing\n                    <i>Escherichia coli<\/i>\n                    Strains Isolated from Diseased Captive Giant Pandas (\n                    <i>Ailuropoda melanoleuca<\/i>\n                    ) in China"],"prefix":"10.1177","volume":"28","author":[{"given":"Xue","family":"Ji","sequence":"first","affiliation":[{"name":"Changchun Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Changchun, China."},{"name":"Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Changchun, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jun","family":"Liu","sequence":"additional","affiliation":[{"name":"Changchun Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Changchun, China."},{"name":"Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Changchun, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bing","family":"Liang","sequence":"additional","affiliation":[{"name":"Changchun Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Changchun, China."},{"name":"Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Changchun, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shiwen","family":"Sun","sequence":"additional","affiliation":[{"name":"Changchun Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Changchun, China."},{"name":"Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Changchun, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lingwei","family":"Zhu","sequence":"additional","affiliation":[{"name":"Changchun Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Changchun, China."},{"name":"Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Changchun, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei","family":"Zhou","sequence":"additional","affiliation":[{"name":"Animal Disease Control Center of Erdos, Erdos, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xuejun","family":"Guo","sequence":"additional","affiliation":[{"name":"Changchun Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Changchun, China."},{"name":"Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Changchun, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yang","family":"Sun","sequence":"additional","affiliation":[{"name":"Changchun Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Changchun, China."},{"name":"Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Changchun, China."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2022,6,10]]},"reference":[{"key":"e_1_3_4_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-3207(02)00255-0"},{"key":"e_1_3_4_3_2","volume-title":"Proc. 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