{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,7]],"date-time":"2026-06-07T01:35:37Z","timestamp":1780796137916,"version":"3.54.1"},"reference-count":38,"publisher":"SAGE Publications","issue":"7","license":[{"start":{"date-parts":[[2022,7,1]],"date-time":"2022-07-01T00:00:00Z","timestamp":1656633600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Microbial Drug Resistance"],"published-print":{"date-parts":[[2022,7,1]]},"abstract":"<jats:p>\n                    Dispersion of transferable oxazolidinone resistance genes among enterococci poses a serious problem to human health. Prompt detection of bacteria carrying these genes is crucial to avoid their spread to multidrug-resistant bacteria. The aim of the study was to describe the presence of\n                    <jats:italic toggle=\"yes\">optrA<\/jats:italic>\n                    -positive isolates among enterococci in a Spanish hospital, and to determine their genetic context and location through whole genome sequencing. All enterococci recovered in a Spanish hospital (Hospital El Bierzo; HEB) from February to December 2018 (\n                    <jats:italic toggle=\"yes\">n<\/jats:italic>\n                    \u2009=\u2009443), with minimal inhibitory concentrations (MICs) to linezolid (LZD) \u22654\u2009mg\/L, were tested by polymerase chain reaction for the presence of\n                    <jats:italic toggle=\"yes\">cfr<\/jats:italic>\n                    ,\n                    <jats:italic toggle=\"yes\">optrA<\/jats:italic>\n                    , and\n                    <jats:italic toggle=\"yes\">poxtA<\/jats:italic>\n                    transferable genes. Only four\n                    <jats:italic toggle=\"yes\">Enterococcus faecalis<\/jats:italic>\n                    isolates (0.9%) had LZD MICs \u22654\u2009mg\/L and none of them was positive for\n                    <jats:italic toggle=\"yes\">cfr<\/jats:italic>\n                    or\n                    <jats:italic toggle=\"yes\">poxtA<\/jats:italic>\n                    genes. However, the\n                    <jats:italic toggle=\"yes\">optrA<\/jats:italic>\n                    gene was detected in three isolates collected from urine samples of community patients, whose genomes were sequenced and subjected to bioinformatics analysis. These isolates belonged to different clones: ST7, ST480, and ST585. In these three isolates, the\n                    <jats:italic toggle=\"yes\">optrA<\/jats:italic>\n                    gene was located on plasmids, associated with IS\n                    <jats:italic toggle=\"yes\">1216<\/jats:italic>\n                    in different arrays. In one isolate, the\n                    <jats:italic toggle=\"yes\">optrA<\/jats:italic>\n                    plasmid coexists with a second plasmid, which carried multiple resistance genes for different classes of antibiotics. Detection of\n                    <jats:italic toggle=\"yes\">optrA<\/jats:italic>\n                    -positive\n                    <jats:italic toggle=\"yes\">E. faecalis<\/jats:italic>\n                    isolates in the community is a matter of concern. The spread of these bacteria into hospital settings, particularly in those, such as the HEB, where vancomycin-resistant enterococci are endemic, should be avoided, to preserve the efficacy of the last-resort oxazolidinones.\n                  <\/jats:p>","DOI":"10.1089\/mdr.2021.0402","type":"journal-article","created":{"date-parts":[[2022,6,21]],"date-time":"2022-06-21T09:18:54Z","timestamp":1655803134000},"page":"773-779","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":15,"title":["Detection of the\n                    <i>optrA<\/i>\n                    Gene Among Polyclonal Linezolid-Susceptible Isolates of\n                    <i>Enterococcus faecalis<\/i>\n                    Recovered from Community Patients"],"prefix":"10.1177","volume":"28","author":[{"given":"Carlos","family":"Rodr\u00edguez-Lucas","sequence":"first","affiliation":[{"name":"Servicio de Microbiolog\u00eda, Hospital Universitario de Cabue\u00f1es, Gij\u00f3n, Spain."},{"name":"Grupo de Microbiolog\u00eda Traslacional. Instituto de Investigaci\u00f3n Sanitaria del Principado de Asturias (ISPA), Oviedo, Spain."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Javier","family":"Fern\u00e1ndez","sequence":"additional","affiliation":[{"name":"Grupo de Microbiolog\u00eda Traslacional. Instituto de Investigaci\u00f3n Sanitaria del Principado de Asturias (ISPA), Oviedo, Spain."},{"name":"Servicio de Microbiolog\u00eda, Hospital Universitario Central de Asturias (HUCA), Oviedo, Spain."},{"name":"Research &amp; Innovation, Artificial Intelligence and Statistical Department, Pragmatech AI Solutions, Oviedo, Spain."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xenia","family":"V\u00e1zquez","sequence":"additional","affiliation":[{"name":"Grupo de Microbiolog\u00eda Traslacional. Instituto de Investigaci\u00f3n Sanitaria del Principado de Asturias (ISPA), Oviedo, Spain."},{"name":"\u00c1rea de Microbiolog\u00eda, Departamento de Biolog\u00eda Funcional, Universidad de Oviedo, Oviedo, Spain."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mar\u00eda","family":"de Toro","sequence":"additional","affiliation":[{"name":"Plataforma de Gen\u00f3mica y Bioinform\u00e1tica, Centro de Investigaci\u00f3n Biom\u00e9dica de La Rioja (CIBIR), Logro\u00f1o, Spain."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"V\u00edctor","family":"Ladero","sequence":"additional","affiliation":[{"name":"Insituto de Productos L\u00e1cteos de Asturias (IPLA-CSIC), Villaviciosa, Spain."},{"name":"Grupo de Microbiolog\u00eda Molecular, Instituto de Investigaci\u00f3n Sanitaria del Principado de Asturias (ISPA), Oviedo, Spain."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carlos","family":"Fuster","sequence":"additional","affiliation":[{"name":"Unidad de Microbiolog\u00eda, Hospital El Bierzo (HEB), Ponferrada, Spain."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rosaura","family":"Rodicio","sequence":"additional","affiliation":[{"name":"Grupo de Microbiolog\u00eda Traslacional. Instituto de Investigaci\u00f3n Sanitaria del Principado de Asturias (ISPA), Oviedo, Spain."},{"name":"Departamento de Bioqu\u00edmica y Biolog\u00eda Molecular, Universidad de Oviedo, Oviedo, Spain."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mar\u00eda Rosario","family":"Rodicio","sequence":"additional","affiliation":[{"name":"Grupo de Microbiolog\u00eda Traslacional. Instituto de Investigaci\u00f3n Sanitaria del Principado de Asturias (ISPA), Oviedo, Spain."},{"name":"\u00c1rea de Microbiolog\u00eda, Departamento de Biolog\u00eda Funcional, Universidad de Oviedo, Oviedo, Spain."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2022,7,13]]},"reference":[{"key":"e_1_3_6_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijantimicag.2003.11.003"},{"key":"e_1_3_6_3_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dky099"},{"key":"e_1_3_6_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.44.9.2530-2533.2000"},{"key":"e_1_3_6_5_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dky088"},{"key":"e_1_3_6_6_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkv116"},{"key":"e_1_3_6_7_2","doi-asserted-by":"publisher","DOI":"10.3390\/microorganisms8081155"},{"key":"e_1_3_6_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01074-19"},{"key":"e_1_3_6_9_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dky188"},{"key":"e_1_3_6_10_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10096-019-03504-3"},{"key":"e_1_3_6_11_2","doi-asserted-by":"publisher","DOI":"10.1089\/mdr.2018.0028"},{"key":"e_1_3_6_12_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkz263"},{"key":"e_1_3_6_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01256-16"},{"key":"e_1_3_6_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijantimicag.2020.105977"},{"key":"e_1_3_6_15_2","doi-asserted-by":"publisher","DOI":"10.1089\/mdr.2021.0171"},{"key":"e_1_3_6_16_2","unstructured":"Clinical and Laboratory Standards Institute (CLSI). 2019. 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