{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T16:57:45Z","timestamp":1791219465846,"version":"4.1.0"},"reference-count":16,"publisher":"SAGE Publications","issue":"10","license":[{"start":{"date-parts":[[2022,10,1]],"date-time":"2022-10-01T00:00:00Z","timestamp":1664582400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Microbial Drug Resistance"],"published-print":{"date-parts":[[2022,10]]},"abstract":"\n An unusual biotype of KPC-2-producing\n Klebsiella pneumoniae<\/jats:italic>\n (KPC-Kpn) isolates was detected in Corrientes, Argentina, which, to their isolation date, had been free of KPC-Kpn outbreaks. Our aim was to describe the clinical epidemiology focused on genomic characterization of atypical urease-negative KPC-Kpn clinical isolates belonging to the high-risk hospital-associated clonal lineage ST340\/CC258. Thirteen isolates were recovered, all of them from inpatients with KPC-Kpn infection (August 2015 to January 2016). These isolates displayed identical enterobacterial repetitive intergenic consensus-PCR electropherotype belonging to a single clonal sequence type ST340. Whole genome sequencing was performed on two KPC-Kpn and the resistome analyses revealed the following acquired resistance genes:\n bla<\/jats:italic>\n KPC-2<\/jats:sub>\n ,\n bla<\/jats:italic>\n CTX-M-15,<\/jats:sub>\n bla<\/jats:italic>\n OXA-1,<\/jats:sub>\n bla<\/jats:italic>\n SHV-11,<\/jats:sub>\n aac(3)-IId<\/jats:italic>\n ,\n aph(3\u2032)-Ia<\/jats:italic>\n ,\n aac(6\u2032)-Ib-cr, sul1<\/jats:italic>\n ,\n dfrA14, catB3, fosA<\/jats:italic>\n , and\n arr-3.<\/jats:italic>\n Mutations in GyrA (S83I) and ParC (S80I) were also identified. Among the virulence determinants, yersiniabactin was detected in both strains, specifically the\n ybt9<\/jats:italic>\n locus located in ICE\n Kp3<\/jats:italic>\n . Five plasmid incompatibility groups were observed in this clone and an unusual IncP6 plasmid bearing\n bla<\/jats:italic>\n KPC-2<\/jats:sub>\n gene (named pKpn3KP) was fully characterized. In this study, we present the first draft genome sequences of two clinical isolates of KPC-2\/CTX-M-15-producing\n K. pneumoniae<\/jats:italic>\n belonging to the high-risk clonal lineage ST340\/CC258 associated with nosocomial outbreaks in Argentina.\n <\/jats:p>","DOI":"10.1089\/mdr.2021.0466","type":"journal-article","created":{"date-parts":[[2022,8,19]],"date-time":"2022-08-19T16:03:36Z","timestamp":1660925016000},"page":"957-961","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":3,"title":["Emergence of Urease-Negative\n Klebsiella pneumoniae<\/i>\n ST340 Carrying an IncP6 Plasmid-Mediated\n bla<\/i>\n KPC-2<\/sub>\n Gene"],"prefix":"10.1177","volume":"28","author":[{"given":"Jos\u00e9","family":"Di Conza","sequence":"first","affiliation":[{"name":"Instituto de Investigaciones en Bacteriolog\u00eda y Virolog\u00eda Molecular (IBaViM), Facultad de Farmacia y Bioqu\u00edmica, Universidad de Buenos Aires, Buenos Aires, Argentina."},{"name":"Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas (CONICET), Buenos Aires, Argentina."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria E.","family":"Badaracco","sequence":"additional","affiliation":[{"name":"Instituto Cardiologico de Corrientes \u201cJuana Francisca Cabral,\u201d Corrientes, Argentina."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanina","family":"Calza","sequence":"additional","affiliation":[{"name":"Instituto Cardiologico de Corrientes \u201cJuana Francisca Cabral,\u201d Corrientes, Argentina."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Herrison","family":"Fontana","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Instituto de Ci\u00eancias Biom\u00e9dicas, Universidade de S\u00e3o Paulo, Sao Paulo, Brazil."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nilton","family":"Lincopan","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Instituto de Ci\u00eancias Biom\u00e9dicas, Universidade de S\u00e3o Paulo, Sao Paulo, Brazil."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura","family":"Pe\u00f1a","sequence":"additional","affiliation":[{"name":"Instituto Cardiologico de Corrientes \u201cJuana Francisca Cabral,\u201d Corrientes, Argentina."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gabriel","family":"Gutkind","sequence":"additional","affiliation":[{"name":"Instituto de Investigaciones en Bacteriolog\u00eda y Virolog\u00eda Molecular (IBaViM), Facultad de Farmacia y Bioqu\u00edmica, Universidad de Buenos Aires, Buenos Aires, Argentina."},{"name":"Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas (CONICET), Buenos Aires, Argentina."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2022,10,14]]},"reference":[{"key":"e_1_3_6_2_2","doi-asserted-by":"publisher","DOI":"10.1093\/femsle\/fnw219"},{"key":"e_1_3_6_3_2","doi-asserted-by":"crossref","first-page":"238","DOI":"10.1016\/j.jgar.2019.06.005","article-title":"Changing epidemiology of KPC-producing Klebsiella pneumoniae in Argentina: emergence of hypermucoviscous ST25 and high-risk clone ST307","volume":"18","author":"Cejas D.","year":"2019","unstructured":"Cejas D., , Elena A., , Guevara Nu\u00f1ez D., et al. 2019. Changing epidemiology of KPC-producing Klebsiella pneumoniae in Argentina: emergence of hypermucoviscous ST25 and high-risk clone ST307. J. Glob. Antimicrob. Resist., 18:238\u2013242.","journal-title":"J. Glob. Antimicrob. Resist."},{"key":"e_1_3_6_4_2","doi-asserted-by":"crossref","first-page":"1520","DOI":"10.1111\/j.1469-0691.2011.03600.x","article-title":"Clonal dissemination of Klebsiella pneumoniae ST258 harbouring KPC-2 in Argentina","volume":"17","author":"Gomez S.A.","year":"2011","unstructured":"Gomez S.A., , Pasteran F.G., , Faccone D., et al. 2011. Clonal dissemination of Klebsiella pneumoniae ST258 harbouring KPC-2 in Argentina. Clin. Microbiol. Infect., 17:1520\u20131524.","journal-title":"Clin. Microbiol. Infect."},{"key":"e_1_3_6_5_2","doi-asserted-by":"crossref","first-page":"499","DOI":"10.1016\/j.meegid.2011.09.018","article-title":"Hyperendemic clone of KPC-producing Klebsiella pneumoniae ST 258 in Buenos Aires hospitals","volume":"12","author":"Cejas D.","year":"2012","unstructured":"Cejas D., , Fernandez Canigia L., , Nastro M., et al. 2012. Hyperendemic clone of KPC-producing Klebsiella pneumoniae ST 258 in Buenos Aires hospitals. Infect. Genet. Evol., 12:499\u2013501.","journal-title":"Infect. Genet. Evol."},{"key":"e_1_3_6_6_2","doi-asserted-by":"publisher","DOI":"10.1111\/nyas.14223"},{"key":"e_1_3_6_7_2","unstructured":"Clinical and Laboratory Standards Institute (CLSI). 2016. Performance Standards for Antimicrobial Susceptibility Testing. 26th ed. CLSI Supplement M100S. Clinical and Laboratory Standards Institute Wayne PA."},{"key":"e_1_3_6_8_2","doi-asserted-by":"crossref","first-page":"114912","DOI":"10.1016\/j.diagmicrobio.2019.114912","article-title":"Performance of distinct phenotypic methods for carbapenemase detection: the influence of culture media","volume":"96","author":"Cordeiro-Moura J.R.","year":"2020","unstructured":"Cordeiro-Moura J.R., , Fehlberg L.C.C., , Nodari C.S., et al. 2020. Performance of distinct phenotypic methods for carbapenemase detection: the influence of culture media. Diagn. Microbiol. Infect. Dis., 96:114912.","journal-title":"Diagn. Microbiol. Infect. Dis."},{"key":"e_1_3_6_9_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/19.24.6823"},{"key":"e_1_3_6_10_2","first-page":"e000196","article-title":"Genetic diversity, mobilisation and spread of the yersiniabactin-encoding mobile element ICEKp in Klebsiella pneumoniae populations","volume":"4","author":"Lam M.M.C.","year":"2018","unstructured":"Lam M.M.C., , Wick R.R, , Wyres K.L, et al. 2018. Genetic diversity, mobilisation and spread of the yersiniabactin-encoding mobile element ICEKp in Klebsiella pneumoniae populations. Microb. Genomics. 4:e000196.","journal-title":"Microb. Genomics"},{"key":"e_1_3_6_11_2","first-page":"310","article-title":"The IncP-6 plasmid p10265-KPC from Pseudomonas aeruginosa carries a novel \u0394ISEc33-associated bla\n KPC-2 gene cluster","volume":"7","author":"Dai X.","year":"2016","unstructured":"Dai X., , Zhou D., , Xiong W., et al. 2016. The IncP-6 plasmid p10265-KPC from Pseudomonas aeruginosa carries a novel \u0394ISEc33-associated blaKPC-2 gene cluster. Front. Microbiol. 7:310.","journal-title":"Front. Microbiol"},{"key":"e_1_3_6_12_2","doi-asserted-by":"crossref","unstructured":"Ghiglione B. Haim M.S Penzotti P. et al. 2021. Characterization of emerging pathogens carrying blaKPC-2 gene in IncP-6 plasmids isolated from urban sewage in Argentina. Front. Cell. Infect. Microbiol. 11:722536.","DOI":"10.3389\/fcimb.2021.722536"},{"key":"e_1_3_6_13_2","doi-asserted-by":"crossref","first-page":"1309","DOI":"10.1590\/S1517-83822012000400010","article-title":"Mutations in the quinolone resistance-determining regions of gyrA and parC in Enterobacteriaceae isolates from Brazil","volume":"43","author":"Minarini L.A.R.","year":"2012","unstructured":"Minarini L.A.R., , and Darini A.L.C. 2012. Mutations in the quinolone resistance-determining regions of gyrA and parC in Enterobacteriaceae isolates from Brazil. Brazilian J. Microbiol. 43:1309\u20131314.","journal-title":"Brazilian J. Microbiol"},{"key":"e_1_3_6_14_2","doi-asserted-by":"publisher","DOI":"10.1089\/mdr.2018.0006"},{"key":"e_1_3_6_15_2","doi-asserted-by":"crossref","first-page":"375","DOI":"10.1016\/j.diagmicrobio.2015.09.003","article-title":"Coproduction of KPC-2 and QnrB19 in Klebsiella pneumoniae ST340 isolate in Brazil","volume":"83","author":"Almeida A.C.S","year":"2015","unstructured":"Martins, W.M.B.S., Almeida A.C.S, , Nicoletti A.G, et al. 2015. Coproduction of KPC-2 and QnrB19 in Klebsiella pneumoniae ST340 isolate in Brazil. Diagn. Microbiol. Infect. Dis. 83:375\u2013376.","journal-title":"Diagn. Microbiol. Infect. 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