{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T19:32:31Z","timestamp":1777923151895,"version":"3.51.4"},"reference-count":25,"publisher":"SAGE Publications","issue":"9","license":[{"start":{"date-parts":[[2022,9,1]],"date-time":"2022-09-01T00:00:00Z","timestamp":1661990400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Microbial Drug Resistance"],"published-print":{"date-parts":[[2022,9]]},"abstract":"<jats:p>\n                    Macrolides are commonly used to control respiratory tract infections in ruminants, but the susceptibility of\n                    <jats:italic toggle=\"yes\">Pasteurella multocida<\/jats:italic>\n                    strains has shown a decrease to macrolide antibiotics in the last decade. In this work we assessed the prevalence of macrolide resistance of 100\n                    <jats:italic toggle=\"yes\">P. multocida<\/jats:italic>\n                    isolates from ruminant hosts and studied the resistance genotypes with newly designed PCRs. Susceptibility to erythromycin and tilmicosin was tested using minimal inhibitory concentration (MIC) test strips. A newly designed PCR was used for the detection of\n                    <jats:italic toggle=\"yes\">macAB<\/jats:italic>\n                    genes, and a PCR plus restriction enzyme-based technique was developed for detecting a 23S rRNA gene mutation at position 2059. Five bovine isolates with notably increased MICs (\u2265256\u2009\u03bcg\/mL for erythromycin and \u226532\u2009\u03bcg\/mL for tilmicosin) carried resistance genes\n                    <jats:italic toggle=\"yes\">msr<\/jats:italic>\n                    (E) and\n                    <jats:italic toggle=\"yes\">mph<\/jats:italic>\n                    (E) or the A2059G point mutation in the 23S rRNA gene. Over 73% strains from small ruminants and all bovine isolates were MacAB PCR positive. Bovine strains were less sensitive to macrolide antibiotics than isolates from small ruminants, and an increase in the prevalence of macrolide resistance in bovine\n                    <jats:italic toggle=\"yes\">P. multocida<\/jats:italic>\n                    isolates has also been observed over time.\n                  <\/jats:p>","DOI":"10.1089\/mdr.2022.0010","type":"journal-article","created":{"date-parts":[[2022,8,15]],"date-time":"2022-08-15T11:16:56Z","timestamp":1660562216000},"page":"941-947","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":3,"title":["Investigation of Macrolide Resistance Genotypes of\n                    <i>Pasteurella multocida<\/i>\n                    Isolates from Cattle and Small Ruminants"],"prefix":"10.1177","volume":"28","author":[{"given":"Barbara","family":"Ujv\u00e1ri","sequence":"first","affiliation":[{"name":"Veterinary Medical Research Institute, Budapest, Hungary."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1632-8874","authenticated-orcid":false,"given":"Tibor","family":"Magyar","sequence":"additional","affiliation":[{"name":"Veterinary Medical Research Institute, Budapest, Hungary."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2022,9,15]]},"reference":[{"key":"e_1_3_6_2_2","doi-asserted-by":"crossref","unstructured":"Wilkie IW Harper M Boyce JD et al. Pasteurella multocida: Diseases and pathogenesis. Curr Top Microbiol Immunol 2012;361:1\u201322; doi: 10.1007\/82_2012_216.","DOI":"10.1007\/82_2012_216"},{"key":"e_1_3_6_3_2","doi-asserted-by":"crossref","unstructured":"Dabo SM Taylor JD Confer AW. Pasteurella multocida and bovine respiratory disease. Anim Health Res Rev 2007;8(2):129\u2013150; doi: 10.1017\/S1466252307001399.","DOI":"10.1017\/S1466252307001399"},{"key":"e_1_3_6_4_2","doi-asserted-by":"crossref","unstructured":"Garc\u00eda-Alvarez A Vela AI San Mart\u00edn E et al. Characterization of Pasteurella multocida associated with ovine pneumonia using multi-locus sequence typing (MLST) and virulence-associated gene profile analysis and comparison with porcine isolates. Vet Microbiol 2017;204:180\u2013187; doi: 10.1016\/j.vetmic.2017.04.015.","DOI":"10.1016\/j.vetmic.2017.04.015"},{"key":"e_1_3_6_5_2","doi-asserted-by":"crossref","unstructured":"Hernando-Amado S Coque TM Baquero F et al. Defining and combating antibiotic resistance from One Health and Global Health perspectives. Nat Microbiol 2019;4:1432\u20131442; doi: 10.1038\/s41564-019-0503-9.","DOI":"10.1038\/s41564-019-0503-9"},{"key":"e_1_3_6_6_2","doi-asserted-by":"crossref","unstructured":"Py\u00f6r\u00e4l\u00e4 S Baptiste KE Catry B et al. Macrolides and lincosamides in cattle and pigs: Use and development of antimicrobial resistance. Vet J 2014;200(2):230\u2013239; doi: 10.1016\/j.tvjl.2014.02.028.","DOI":"10.1016\/j.tvjl.2014.02.028"},{"key":"e_1_3_6_7_2","doi-asserted-by":"crossref","unstructured":"Desmolaize B Rose S Warrass R et al. A novel Erm monomethyltransferase in antibiotic-resistant isolates of Mannheimia haemolytica and Pasteurella multocida. Mol Microbiol 2011;80(1):184\u2013194; doi: 10.1111\/j.1365-2958.2011.07567.x.","DOI":"10.1111\/j.1365-2958.2011.07567.x"},{"key":"e_1_3_6_8_2","doi-asserted-by":"crossref","unstructured":"Desmolaize B Rose S Wilhelm C et al. Combinations of macrolide resistance determinants in field isolates of Mannheimia haemolytica and Pasteurella multocida. Antimicrob Agents Chemother 2011;55(9):4128\u20134133; doi: 10.1128\/AAC.00450-11.","DOI":"10.1128\/AAC.00450-11"},{"key":"e_1_3_6_9_2","doi-asserted-by":"crossref","unstructured":"Michael GB Eidam C Kadlec K et al. Increased MICs of gamithromycin and tildipirosin in the presence of the genes erm(42) and msr(E)-mph(E) for bovine Pasteurella multocida and Mannheimia haemolytica. J Antimicrob Chemother 2012;67(6):1555\u20131557; doi: 10.1093\/jac\/dks076.","DOI":"10.1093\/jac\/dks076"},{"key":"e_1_3_6_10_2","doi-asserted-by":"crossref","unstructured":"Kadlec K Brenner Michael G Sweeney MT et al. Molecular basis of macrolide triamilide and lincosamide resistance in Pasteurella multocida from bovine respiratory disease. Antimicrob Agents Chemother 2011;55(5):2475\u20132477; doi: 10.1128\/AAC.00092-11.","DOI":"10.1128\/AAC.00092-11"},{"key":"e_1_3_6_11_2","doi-asserted-by":"crossref","unstructured":"Rose S Desmolaize B Jaju P et al. Multiplex PCR to identify macrolide resistance determinants in Mannheimia haemolytica and Pasteurella multocida. Antimicrob Agents Chemother 2012;56(7):3664\u20133669; doi: 10.1128\/AAC.00266-12.","DOI":"10.1128\/AAC.00266-12"},{"key":"e_1_3_6_12_2","doi-asserted-by":"crossref","unstructured":"Alhamami T Chowdhury PR Gomes N et al. First emergence of resistance to macrolides and tetracycline identified in Mannheimia haemolytica and Pasteurella multocida isolates from beef feedlots in Australia. Microorganisms 2021;9(6):1322; doi: 10.3390\/microorganisms9061322.","DOI":"10.3390\/microorganisms9061322"},{"key":"e_1_3_6_13_2","doi-asserted-by":"crossref","unstructured":"Olsen AS Warrass R Douthwaite S. Macrolide resistance conferred by rRNA mutations in field isolates of Mannheimia haemolytica and Pasteurella multocida. J Antimicrob Chemother 2015;70(2):420\u2013423; doi: 10.1093\/jac\/dku385.","DOI":"10.1093\/jac\/dku385"},{"key":"e_1_3_6_14_2","doi-asserted-by":"crossref","unstructured":"Dayao DAE Seddon JM Gibson JS et al. Whole genome sequence analysis of pig respiratory bacterial pathogens with elevated minimum inhibitory concentrations for macrolides. Microb Drug Resist 2016;22(7):531\u2013537; doi: 10.1089\/mdr.2015.0214.","DOI":"10.1089\/mdr.2015.0214"},{"key":"e_1_3_6_15_2","doi-asserted-by":"crossref","unstructured":"Kobayashi N Nishino K Yamaguchi A. Novel macrolide-specific ABC-type efflux transporter in Escherichia coli. J Bacteriol 2001;183(19):5639\u20135644; doi: 10.1128\/JB.183.19.5639-5644.2001.","DOI":"10.1128\/JB.183.19.5639-5644.2001"},{"key":"e_1_3_6_16_2","doi-asserted-by":"crossref","unstructured":"Okada U Yamashita E Neuberger A et al. 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