{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T19:32:29Z","timestamp":1777923149541,"version":"3.51.4"},"reference-count":23,"publisher":"SAGE Publications","issue":"11","license":[{"start":{"date-parts":[[2022,11,1]],"date-time":"2022-11-01T00:00:00Z","timestamp":1667260800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Microbial Drug Resistance"],"published-print":{"date-parts":[[2022,11]]},"abstract":"<jats:p>\n                    Typing carbapenem-resistant\n                    <jats:italic toggle=\"yes\">Klebsiella pneumoniae<\/jats:italic>\n                    (CR-KPN) is crucial in controlling their dissemination and solving outbreaks. In this context, we searched for an effective, faster, and cheaper alternative technique to type KPN by analyzing the\n                    <jats:italic toggle=\"yes\">fosA<\/jats:italic>\n                    <jats:sup>KP<\/jats:sup>\n                    sequence. We analyzed the nucleotide sequences of chromosomal\n                    <jats:italic toggle=\"yes\">fosA<\/jats:italic>\n                    <jats:sup>KP<\/jats:sup>\n                    gene in 350 KPN genomes (70 per sequence type [ST] or clonal complex [CC]). Assembly genomes were randomly downloaded from NCBI and annotated using RAST in PATRIC platform. The isolate STs were verified using multilocus sequence typing 2.0 by the Center for Genomic Epidemiology. Chromosomally encoded\n                    <jats:italic toggle=\"yes\">fosA<\/jats:italic>\n                    <jats:sup>KP<\/jats:sup>\n                    was confirmed in MLplasmid, and the sequence alignments were performed in Clustal Omega. The amino acid sequences were analyzed using SNAP2 and SMART platforms. Out of the 70 genomes analyzed for each ST\/CC, we observed 100%\n                    <jats:italic toggle=\"yes\">fosA<\/jats:italic>\n                    sequence identity for CC258\/11, ST15, ST307, and ST101. For ST16, only two\n                    <jats:italic toggle=\"yes\">fosA<\/jats:italic>\n                    sequences were different from each other. We observed differences in amino acid sequences at positions 25 and 79 (ST16) and 86 (ST16, ST101). The C-terminal (amino acid 138, 139, 140) was different for each cluster. None of these polymorphisms is related to the protein active site. Moreover, L25Q (ST16) polymorphism was predicted to probably affect the protein function. We observed that chromosomal\n                    <jats:italic toggle=\"yes\">fosA<\/jats:italic>\n                    <jats:sup>KP<\/jats:sup>\n                    sequences from KPN are highly conserved in ST15, ST307, ST16, ST101, and CC258\/11, suggesting\n                    <jats:italic toggle=\"yes\">fosA<\/jats:italic>\n                    <jats:sup>KP<\/jats:sup>\n                    sequencing as an alternative, easier, faster, and less expensive technique in identifying epidemiological STs for KPN, and discriminating them from CC258\/11.\n                  <\/jats:p>","DOI":"10.1089\/mdr.2022.0081","type":"journal-article","created":{"date-parts":[[2022,11,1]],"date-time":"2022-11-01T17:19:55Z","timestamp":1667323195000},"page":"1037-1042","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":1,"title":["Sequencing of\n                    <i>fosA<\/i>\n                    : A Rapid and Inexpensive Method for Discriminating\n                    <i>Klebsiella pneumoniae<\/i>\n                    CC258 from Other Clones"],"prefix":"10.1177","volume":"28","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4997-0505","authenticated-orcid":false,"given":"\u00c1ghata Cardoso da Silva","family":"Ribeiro","sequence":"first","affiliation":[{"name":"Laborat\u00f3rio Alerta, Division of Infectious Diseases, Department of Internal Medicine, Escola Paulista de Medicina\/Universidade Federal de S\u00e3o Paulo (UNIFESP), S\u00e3o Paulo, S\u00e3o Paulo, Brazil."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fernanda Fernandes","family":"Santos","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio Alerta, Division of Infectious Diseases, Department of Internal Medicine, Escola Paulista de Medicina\/Universidade Federal de S\u00e3o Paulo (UNIFESP), S\u00e3o Paulo, S\u00e3o Paulo, Brazil."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ikechukwu Benjamin","family":"Moses","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio Alerta, Division of Infectious Diseases, Department of Internal Medicine, Escola Paulista de Medicina\/Universidade Federal de S\u00e3o Paulo (UNIFESP), S\u00e3o Paulo, S\u00e3o Paulo, Brazil."},{"name":"Department of Applied Microbiology, Faculty of Science, Ebonyi State University, Abakaliki, Nigeria."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luciene Andrade da Rocha","family":"Minarini","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio Multidisciplinar em Sa\u00fade e Meio Ambiente, Departamento de Ci\u00eancias Farmac\u00eauticas, Instituto de Ci\u00eancias Ambientais, Qu\u00edmicas e Farmac\u00eauticas, Universidade Federal de S\u00e3o Paulo (UNIFESP), Diadema, S\u00e3o Paulo, Brazil."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0913-768X","authenticated-orcid":false,"given":"Ana Cristina","family":"Gales","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio Alerta, Division of Infectious Diseases, Department of Internal Medicine, Escola Paulista de Medicina\/Universidade Federal de S\u00e3o Paulo (UNIFESP), S\u00e3o Paulo, S\u00e3o Paulo, Brazil."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2022,11,14]]},"reference":[{"key":"e_1_3_6_2_2","doi-asserted-by":"crossref","first-page":"420","DOI":"10.1016\/j.jgar.2020.10.016","article-title":"Global genotype distribution of human clinical isolates of New Delhi metallo-\u03b2-lactamase-producing Klebsiella pneumoniae; A systematic review","volume":"23","author":"Safavi M","year":"2020","unstructured":"Safavi M, , Bostanshirin N, , Hajikhani B, et al. Global genotype distribution of human clinical isolates of New Delhi metallo-\u03b2-lactamase-producing Klebsiella pneumoniae; A systematic review. J Glob Antimicrob Resist, 2020; 23:420\u2013429; doi:10.1016\/j.jgar.2020.10.016","journal-title":"J Glob Antimicrob Resist"},{"key":"e_1_3_6_3_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2016.00895"},{"issue":"7","key":"e_1_3_6_4_2","doi-asserted-by":"crossref","first-page":"e141","DOI":"10.1093\/cid\/ciz1095","article-title":"An emerging clone, Klebsiella pneumoniae carbapenemase 2-producing K. pneumoniae sequence type 16, associated with high mortality rates in a CC258-endemic setting","volume":"71","author":"Andrey DO","year":"2020","unstructured":"Andrey DO, , Dantas PP, , Martins WBS, et al. An emerging clone, Klebsiella pneumoniae carbapenemase 2-producing K. pneumoniae sequence type 16, associated with high mortality rates in a CC258-endemic setting. Clin Infect Dis, 2020; 71(7):e141\u2013e150; doi: 10.1093\/cid\/ciz1095","journal-title":"Clin Infect Dis"},{"issue":"3","key":"e_1_3_6_5_2","doi-asserted-by":"crossref","first-page":"e0014822","DOI":"10.1128\/spectrum.00148-22","article-title":"Extensively drug-resistant Klebsiella pneumoniae counteracts fitness and virulence costs that accompanied ceftazidime-avibactam resistance acquisition","volume":"10","author":"Eger E","year":"2022","unstructured":"Eger E, , Schwabe M, , Schulig L, et al. Extensively drug-resistant Klebsiella pneumoniae counteracts fitness and virulence costs that accompanied ceftazidime-avibactam resistance acquisition. Microbiol Spectr, 2022; 10(3):e0014822; doi:10.1128\/spectrum.00148-22","journal-title":"Microbiol Spectr"},{"key":"e_1_3_6_6_2","doi-asserted-by":"crossref","first-page":"635206","DOI":"10.3389\/fmed.2021.635206","article-title":"Genetic basis of antimicrobial resistant gram-negative bacteria isolated from bloodstream in Brazil","volume":"8","author":"Silveira MC","year":"2021","unstructured":"Silveira MC, , Rocha-de-Souza CM, , de Oliveira Santos IC, et al. Genetic basis of antimicrobial resistant gram-negative bacteria isolated from bloodstream in Brazil. Front Med, 2021; 8:635206; doi: 10.3389\/fmed.2021.635206","journal-title":"Front Med"},{"key":"e_1_3_6_7_2","doi-asserted-by":"crossref","first-page":"1563","DOI":"10.3389\/fmicb.2020.01563","article-title":"Carbapenemase-producing Klebsiella pneumoniae from transplanted patients in brazil: phylogeny, resistome, virulome and mobile genetic elements harboring blaKPC\u20132 or blaNDM\u20131","volume":"11","author":"Raro OHF","year":"2020","unstructured":"Raro OHF, , da Silva RMC, , Filho EMR, et al. Carbapenemase-producing Klebsiella pneumoniae from transplanted patients in brazil: phylogeny, resistome, virulome and mobile genetic elements harboring blaKPC\u20132 or blaNDM\u20131. Front Microbiol, 2020; 11:1563; doi:10.3389\/fmicb.2020.01563","journal-title":"Front Microbiol"},{"key":"e_1_3_6_8_2","unstructured":"PEpidemiological Alert AHO . Emergence and incrase of new combinations of carbapenemases in Enterobacterales in Latin America and Caribbean. the. 2021. Available from: https:\/\/www.pahoOrg\/En\/Documents\/Epidemiological-Alert-Emergence-and-Increase-New-Combinations-Carbapenemases [Last accessed: March 2022]."},{"issue":"4","key":"e_1_3_6_9_2","first-page":"e00749","article-title":"Widespread fosfomycin resistance in gram-negative bacteria attributable to the chromosomal fosA gene","volume":"8","author":"Ito R","year":"2017","unstructured":"Ito R, , Mustapha MM, , Tomich AD, et al. Widespread fosfomycin resistance in gram-negative bacteria attributable to the chromosomal fosA gene. MBio, 2017; 8(4):e00749-17; doi:10.1128\/mbio.00749-17","journal-title":"MBio"},{"issue":"5","key":"e_1_3_6_10_2","doi-asserted-by":"crossref","first-page":"e00150","DOI":"10.1128\/AAC.00150-20","article-title":"ISEcp1-mediated transposition leads to fosfomycin and broad-spectrum cephalosporin resistance in Klebsiella pneumoniae","volume":"64","author":"Kieffer N","year":"2020","unstructured":"Kieffer N, , Poirel L, , Mueller L, et al. ISEcp1-mediated transposition leads to fosfomycin and broad-spectrum cephalosporin resistance in Klebsiella pneumoniae. Antimicrob Agents Chemother, 2020; 64(5):e00150-20; doi:10.1128\/AAC.00150-20","journal-title":"Antimicrob Agents Chemother"},{"key":"e_1_3_6_11_2","doi-asserted-by":"crossref","first-page":"1273","DOI":"10.2147\/IDR.S251360","article-title":"Identification of FOSA10, a novel plasmid-mediated fosfomycin resistance gene of klebsiella pneumoniae origin, in Escherichia coli","volume":"13","author":"Huang Y","year":"2020","unstructured":"Huang Y, , Lin Q, , Zhou Q, et al. Identification of FOSA10, a novel plasmid-mediated fosfomycin resistance gene of klebsiella pneumoniae origin, in Escherichia coli. 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