{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T19:32:30Z","timestamp":1777923150789,"version":"3.51.4"},"reference-count":32,"publisher":"SAGE Publications","issue":"3","license":[{"start":{"date-parts":[[2023,3,1]],"date-time":"2023-03-01T00:00:00Z","timestamp":1677628800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Microbial Drug Resistance"],"published-print":{"date-parts":[[2023,3,1]]},"abstract":"<jats:p>\n                    A real-time PCR assay based on probe fluorescence quenching by a primer was developed and validated for detection of macrolide resistance (MR)-associated mutations in the 23S rRNA gene of\n                    <jats:italic toggle=\"yes\">Mycoplasma genitalium.<\/jats:italic>\n                    The assay involves identification of any nucleotide substitutions at positions 2058, 2059, and 2611 of 23S rRNA (\n                    <jats:italic toggle=\"yes\">Escherichia coli<\/jats:italic>\n                    numbering) by the probe-based melting curve analysis immediately after amplification and was capable of detecting target mutations in clinical specimens and spiked samples with 92% sensitivity and 100% specificity. We applied this new assay to assess the prevalence of MR-associated mutations in 949 nonduplicate urogenital samples positive for\n                    <jats:italic toggle=\"yes\">M. genitalium<\/jats:italic>\n                    by routine diagnostic PCR, which were collected from symptomatic patients in five cities in the European part of Russia during the period 2009\u20132019. Forty-three (4.92%) samples revealed the presence of MR mutations, and no trend toward an increase in resistance prevalence was observed over the 10-year period of the study. The most commonly detected mutations were A2058G (26\/43; 60.47%) and A2059G (13\/43; 30.23%), while other mutations were rare: A2058T (3\/43; 6.98%) and C2611T (1\/43; 2.33%). The data obtained underline the need for regular epidemiological monitoring to ensure effective patient management, rational use of antibiotics, and prevention of further spread of resistance.\n                  <\/jats:p>","DOI":"10.1089\/mdr.2022.0131","type":"journal-article","created":{"date-parts":[[2023,3,17]],"date-time":"2023-03-17T11:44:26Z","timestamp":1679053466000},"page":"69-77","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":5,"title":["Development of a Real-Time PCR Assay for Detection of Macrolide Resistance Mutations in\n                    <i>Mycoplasma genitalium<\/i>\n                    and Its Application for Epidemiological Surveillance in Russia"],"prefix":"10.1177","volume":"29","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3720-1004","authenticated-orcid":false,"given":"Inna Alexandrovna","family":"Edelstein","sequence":"first","affiliation":[{"name":"Laboratory of Molecular Diagnostics, Institute of Antimicrobial Chemotherapy, Federal State Budgetary Educational Institution of Higher Education \u201cSmolensk State Medical University\u201d of the Ministry of Health of the Russian Federation, Smolensk, Russia."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2223-193X","authenticated-orcid":false,"given":"Andrey Vyacheslavovich","family":"Romanov","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Diagnostics, Institute of Antimicrobial Chemotherapy, Federal State Budgetary Educational Institution of Higher Education \u201cSmolensk State Medical University\u201d of the Ministry of Health of the Russian Federation, Smolensk, Russia."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8728-1113","authenticated-orcid":false,"given":"Roman Sergeevich","family":"Kozlov","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Diagnostics, Institute of Antimicrobial Chemotherapy, Federal State Budgetary Educational Institution of Higher Education \u201cSmolensk State Medical University\u201d of the Ministry of Health of the Russian Federation, Smolensk, Russia."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2023,3,16]]},"reference":[{"key":"e_1_3_5_2_2","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.000830"},{"key":"e_1_3_5_3_2","doi-asserted-by":"publisher","DOI":"10.1177\/0956462414539464"},{"key":"e_1_3_5_4_2","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/ciy025"},{"key":"e_1_3_5_5_2","doi-asserted-by":"publisher","DOI":"10.1177\/0956462416648585"},{"key":"e_1_3_5_6_2","doi-asserted-by":"publisher","DOI":"10.1111\/jdv.17972"},{"key":"e_1_3_5_7_2","doi-asserted-by":"publisher","DOI":"10.2340\/00015555-0929"},{"key":"e_1_3_5_8_2","doi-asserted-by":"publisher","DOI":"10.15585\/mmwr.rr7004a1"},{"key":"e_1_3_5_9_2","unstructured":"Federal Clinical Guidelines. Russian Society of Dermatovenerologists and Cosmetologists. Skin Diseases. Sexually Transmitted Infections 5th ed. Revised and Additional. Business Express: Moscow Russia; 2016."},{"key":"e_1_3_5_10_2","doi-asserted-by":"publisher","DOI":"10.1136\/sti.79.4.318"},{"key":"e_1_3_5_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijantimicag.2020.105911"},{"key":"e_1_3_5_12_2","doi-asserted-by":"publisher","DOI":"10.1136\/bmjopen-2021-050475"},{"key":"e_1_3_5_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01084-20"},{"key":"e_1_3_5_14_2","doi-asserted-by":"publisher","DOI":"10.1177\/0956462419890737"},{"key":"e_1_3_5_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(20)30154-7"},{"key":"e_1_3_5_16_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0170773"},{"key":"e_1_3_5_17_2","doi-asserted-by":"publisher","DOI":"10.1136\/sextrans-2019-054124"},{"key":"e_1_3_5_18_2","doi-asserted-by":"publisher","DOI":"10.1111\/jdv.13849"},{"issue":"2","key":"e_1_3_5_19_2","first-page":"137","article-title":"Data handling as a basis for local antimicrobial resistance surveillance","volume":"22","author":"Vinogradova A","year":"2020","unstructured":"Vinogradova A, , Kuzmenkov A. Data handling as a basis for local antimicrobial resistance surveillance. Kliniceskaa Mikrobiologia i Antimikrobnaa Himioterapia, 2020; 22(2):137\u2013141; doi: 10.36488\/cmac.2020.2.137\u2013141","journal-title":"Kliniceskaa Mikrobiologia i Antimikrobnaa Himioterapia"},{"issue":"2","key":"e_1_3_5_20_2","first-page":"119","article-title":"AMRcloud: A new paradigm in monitoring of antibiotic resistance","volume":"21","author":"Kuzmenkov A","year":"2019","unstructured":"Kuzmenkov A, , Vinogradova A, , Trushin I, et al. AMRcloud: A new paradigm in monitoring of antibiotic resistance. Kliniceskaa Mikrobiologia i Antimikrobnaa Himioterapia, 2019; 21(2):119\u2013124; doi: 10.36488\/cmac.2019.2.119\u2013124","journal-title":"Kliniceskaa Mikrobiologia i Antimikrobnaa Himioterapia"},{"key":"e_1_3_5_21_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.42.2.683-692.2004"},{"key":"e_1_3_5_22_2","doi-asserted-by":"publisher","DOI":"10.1086\/593188"},{"key":"e_1_3_5_23_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkw584"},{"key":"e_1_3_5_24_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkv136"},{"key":"e_1_3_5_25_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dks263"},{"key":"e_1_3_5_26_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12879-018-3523-9"},{"key":"e_1_3_5_27_2","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/cit752"},{"key":"e_1_3_5_28_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00495-13"},{"key":"e_1_3_5_29_2","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/ciu217"},{"key":"e_1_3_5_30_2","volume-title":"Mycoplasma genitalium","author":"Twin J","unstructured":"Twin J, , Jensen J, , Bradshaw C, et al. Transmission and selection of macrolide resistant Mycoplasma genitalium. infections detected by rapid high resolution melt analysis. Kaltenboeck B, ed. PLoS One 2012;7(4): e35593; doi: 10.1371\/journal.pone.003, 5593"},{"key":"e_1_3_5_31_2","author":"Lewis D","year":"2015","unstructured":"Lewis D, Couldwell D. Mycoplasma genitalium infection: Current treatment options, therapeutic failure, and resistance-associated mutations. Infect Drug Resist May 2015:147; doi: 10.2147\/IDR.S48813","journal-title":"Infect Drug Resist"},{"key":"e_1_3_5_32_2","author":"Xiao L","year":"2022","unstructured":"Xiao L, , Totten A, , Crabb D, et al. Antimicrobial susceptibilities and mechanisms of resistance of commensal and invasive Mycoplasma salivarium isolates. 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