{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,15]],"date-time":"2026-05-15T10:11:07Z","timestamp":1778839867782,"version":"3.51.4"},"reference-count":36,"publisher":"SAGE Publications","issue":"2","license":[{"start":{"date-parts":[[2023,10,19]],"date-time":"2023-10-19T00:00:00Z","timestamp":1697673600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Microbial Drug Resistance"],"published-print":{"date-parts":[[2026,2,1]]},"abstract":"<jats:sec>\n                    <jats:title>Aims:<\/jats:title>\n                    <jats:p>\n                      <jats:italic toggle=\"yes\">Staphylococcus pseudintermedius<\/jats:italic>\n                      is an opportunistic pathogen also indicated as one of the major causes of skin infections in dogs. This study aimed to identify\n                      <jats:italic toggle=\"yes\">S. pseudintermedius<\/jats:italic>\n                      isolated from canine skin lesions, evaluate their antibiotic resistance profile and biofilm production ability.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methodology:<\/jats:title>\n                    <jats:p>\n                      Lesions from 50 rural dogs with different skin lesions were sampled after pyoderma diagnosis by private practices. Bacterial species determination was investigated and susceptibility to nine antimicrobials were determined by means of Kirby\u2013Bauer assay. Then seven antibiotic resistance genes, including\n                      <jats:italic toggle=\"yes\">\n                        mecA, blaZ, tetK, tetM, bla\n                        <jats:sub>SHV<\/jats:sub>\n                        , bla\n                        <jats:sub>OXA-<\/jats:sub>\n                      <\/jats:italic>\n                      <jats:sub>1<\/jats:sub>\n                      , and\n                      <jats:italic toggle=\"yes\">\n                        bla\n                        <jats:sub>TEM<\/jats:sub>\n                      <\/jats:italic>\n                      were screened by PCR. Moreover, biofilm formation ability of the strains was determined using the microtiter plate assay along with the presence of\n                      <jats:italic toggle=\"yes\">icaADBC<\/jats:italic>\n                      genes.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results:<\/jats:title>\n                    <jats:p>\n                      A total of 37 (74%) isolates were identified as\n                      <jats:italic toggle=\"yes\">S. pseudintermedius.<\/jats:italic>\n                      All\n                      <jats:italic toggle=\"yes\">S. pseudintermedius<\/jats:italic>\n                      isolates were resistant to multiple drugs. Resistance to penicillin, amoxicillin\u2013clavulanic acid, and cefazolin were higher than other antimicrobials. All the beta-lactam-resistant isolates carried\n                      <jats:italic toggle=\"yes\">blaZ<\/jats:italic>\n                      , whereas\n                      <jats:italic toggle=\"yes\">mecA<\/jats:italic>\n                      was found in 6 (16.21%) of them. Among tetracycline-resistant strains, the frequency of\n                      <jats:italic toggle=\"yes\">tetK<\/jats:italic>\n                      and\n                      <jats:italic toggle=\"yes\">tetM<\/jats:italic>\n                      determinants were 19 (90.47%) and 21 (100%), respectively. Finally, most cefazolin-resistant strains 31 (91.89%) were positive for\n                      <jats:italic toggle=\"yes\">blaTEM<\/jats:italic>\n                      gene. The rate of biofilm production ability among\n                      <jats:italic toggle=\"yes\">S. pseudintermedius<\/jats:italic>\n                      isolates was 35 (94.59%). Furthermore, the presence of\n                      <jats:italic toggle=\"yes\">icaA\/D<\/jats:italic>\n                      genes was detected in 35 (100%) and 29 (82.85%) of\n                      <jats:italic toggle=\"yes\">S. pseudintermedius<\/jats:italic>\n                      strains, respectively.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion:<\/jats:title>\n                    <jats:p>\n                      The high rates of antimicrobial resistance and biofilm production ability among\n                      <jats:italic toggle=\"yes\">S. pseudintermedius<\/jats:italic>\n                      isolates, emphasize the urgent need to use more effective infection control policies and guidelines for antimicrobial use.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1089\/mdr.2022.0257","type":"journal-article","created":{"date-parts":[[2023,10,19]],"date-time":"2023-10-19T11:09:04Z","timestamp":1697713744000},"page":"41-48","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":3,"title":["Detection of Antimicrobial Resistance and Biofilm Production Among\n                    <i>Staphylococcus pseudintermedius<\/i>\n                    from Canine Skin Lesions"],"prefix":"10.1177","volume":"32","author":[{"given":"Sahar Nouri","family":"Gharajalar","sequence":"first","affiliation":[{"name":"Department of Pathobiology, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Iran."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sadegh","family":"Tanhaee","sequence":"additional","affiliation":[{"name":"Master Student of Veterinary Bacteriology, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Iran."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mahdieh","family":"Omidzadeh","sequence":"additional","affiliation":[{"name":"Master Student of Veterinary Bacteriology, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Iran."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masoud","family":"Onsori","sequence":"additional","affiliation":[{"name":"Private Veterinary Practitioner, Urmia, Iran."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2023,10,19]]},"reference":[{"key":"e_1_3_6_2_2","first-page":"1021","article-title":"Antimicrobial resistance in Staphylococcus pseudintermedius and the molecular epidemiology of methicillin-resistant S. pseudintermedius in small animals in Finland","volume":"72","author":"Grontal T","year":"2017","unstructured":"Grontal T, , Eklund M, , Thomson K, et al. 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