{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T19:32:43Z","timestamp":1777923163343,"version":"3.51.4"},"reference-count":21,"publisher":"SAGE Publications","issue":"9","license":[{"start":{"date-parts":[[2023,8,28]],"date-time":"2023-08-28T00:00:00Z","timestamp":1693180800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Microbial Drug Resistance"],"published-print":{"date-parts":[[2023,9]]},"abstract":"\n To investigate the occurrence of oxazolidinone resistance genes, 18 florfenicol-resistant enterococci were isolated from 66 fecal samples collected from several cattle farms in central Italy. The PCR screening indicated that only a bovine florfenicol-resistant isolate,\n Enterococcus faecium<\/jats:italic>\n 249031-C, was positive for the presence of\n optrA<\/jats:italic>\n and\n poxtA<\/jats:italic>\n genes. The strain was tested for its susceptibility to florfenicol, chloramphenicol, linezolid, tedizolid, tetracycline, erythromycin, and vancomycin. Whole Genome Sequencing analysis showed that\n E. faecium<\/jats:italic>\n 249031-C, belonging to the ST22 lineage, harbored two plasmids: the\n optrA<\/jats:italic>\n -carrying p249031-S (179\u2009kb) and the\n poxtA<\/jats:italic>\n -carrying p1818-c (23\u2009kb). p249031-S, containing a new\n optrA<\/jats:italic>\n -carrying Tn\n 7695<\/jats:italic>\n transposon, was closely related to the plasmid pF88_1 of\n E. faecium<\/jats:italic>\n F88, whereas p1818-c had already been detected in a human\n E. faecium<\/jats:italic>\n , both enterococci were from Switzerland. The linezolid resistance genes were cotransferred to the\n E. faecium<\/jats:italic>\n 64\/3 recipient. Circular forms from both\n optrA<\/jats:italic>\n - and\n poxtA<\/jats:italic>\n -carrying genetic contexts were obtained. The occurrence of oxazolidinone resistance genes in a bovine\n E. faecium<\/jats:italic>\n isolate and their localization on conjugative and mobilizable plasmids pose a risk for public health.\n <\/jats:p>","DOI":"10.1089\/mdr.2023.0055","type":"journal-article","created":{"date-parts":[[2023,7,31]],"date-time":"2023-07-31T12:46:31Z","timestamp":1690807591000},"page":"438-442","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":4,"title":["An\n Enterococcus faecium<\/i>\n Isolated from Bovine Feces in Italy Shares\n optrA<\/i>\n - and\n poxtA<\/i>\n -Carrying Plasmids with Enterococci from Switzerland"],"prefix":"10.1177","volume":"29","author":[{"given":"Marzia","family":"Cinthi","sequence":"first","affiliation":[{"name":"Department of Life and Environmental Sciences and Polytechnic University of Marche, Ancona, Italy."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sonia Nina","family":"Coccitto","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences and Public Health, Polytechnic University of Marche, Ancona, Italy."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Serena","family":"Simoni","sequence":"additional","affiliation":[{"name":"Department of Life and Environmental Sciences and Polytechnic University of Marche, Ancona, Italy."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carla","family":"Vignaroli","sequence":"additional","affiliation":[{"name":"Department of Life and Environmental Sciences and Polytechnic University of Marche, Ancona, Italy."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andrea","family":"Brenciani","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences and Public Health, Polytechnic University of Marche, Ancona, Italy."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eleonora","family":"Giovanetti","sequence":"additional","affiliation":[{"name":"Department of Life and Environmental Sciences and Polytechnic University of Marche, Ancona, Italy."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2023,8,28]]},"reference":[{"issue":"36","key":"e_1_3_5_2_2","doi-asserted-by":"crossref","first-page":"13339","DOI":"10.1073\/pnas.0804276105","article-title":"The oxazolidinone antibiotics perturb the ribosomal peptidyl-transferase center and effect tRNA positioning","volume":"105","author":"Wilson DN","year":"2008","unstructured":"Wilson DN, , Schluenzen F, , Harms JM, et al. The oxazolidinone antibiotics perturb the ribosomal peptidyl-transferase center and effect tRNA positioning. Proc Natl Acad Sci USA, 2008; 105(36):13339\u201313344; doi: 10.1073\/pnas.0804276105","journal-title":"Proc Natl Acad Sci USA"},{"issue":"10","key":"e_1_3_5_3_2","doi-asserted-by":"crossref","first-page":"2596","DOI":"10.1093\/jac\/dkac263","article-title":"Oxazolidinones: Mechanisms of resistance and mobile genetic elements involved","volume":"77","author":"Brenciani A","year":"2022","unstructured":"Brenciani A, , Morroni G, , Schwarz S, et al. Oxazolidinones: Mechanisms of resistance and mobile genetic elements involved. 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Microorganisms, 2020; 8(12):2021; doi: 10.3390\/microorganisms8122021","journal-title":"Microorganisms"},{"issue":"3","key":"e_1_3_5_7_2","doi-asserted-by":"crossref","first-page":"e0018820","DOI":"10.1128\/CMR.00188-20","article-title":"Mobile oxazolidinone resistance genes in Gram-positive and Gram-negative bacteria","volume":"34","author":"Schwarz S","year":"2021","unstructured":"Schwarz S, , Zhang W, , Du XD, et al. Mobile oxazolidinone resistance genes in Gram-positive and Gram-negative bacteria. Clin Microbiol Rev, 2021; 34(3):e0018820; doi: 10.1128\/CMR.00188-20","journal-title":"Clin Microbiol Rev"},{"issue":"9","key":"e_1_3_5_8_2","doi-asserted-by":"crossref","first-page":"e02958","DOI":"10.1128\/AEM.02958-20","article-title":"Linezolid resistance genes in enterococci isolated from sediment and zooplankton in two Italian coastal areas","volume":"87","author":"Fioriti S","year":"2021","unstructured":"Fioriti S, , Coccitto SN, , Cedraro N, et al. 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