{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,5]],"date-time":"2026-09-05T00:36:21Z","timestamp":1788568581529,"version":"build-2803163510"},"reference-count":17,"publisher":"SAGE Publications","issue":"5","license":[{"start":{"date-parts":[[2024,5,1]],"date-time":"2024-05-01T00:00:00Z","timestamp":1714521600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Microbial Drug Resistance"],"published-print":{"date-parts":[[2024,5]]},"abstract":"<jats:p>\n                    There is an emerging fluconazole resistance in\n                    <jats:italic toggle=\"yes\">Candida parapsilosis<\/jats:italic>\n                    in recent years. The leading mechanism causing azole resistance in\n                    <jats:italic toggle=\"yes\">C. parapsilosis<\/jats:italic>\n                    is the Y132F codon alteration in the ERG11 gene which encodes the target enzyme of azole drugs. In this study, we evaluated the sensitivity, compatibility, and specificity of a novel tetra-primer amplification refractory mutation system-polymerase chain reaction (T-ARMS-PCR) method for rapid detection of the Y132F mutation in fluconazole nonsusceptible\n                    <jats:italic toggle=\"yes\">C. parapsilosis.<\/jats:italic>\n                    Antifungal susceptibility tests for detection of fluconazole resistance were performed by broth microdilution according to the CLSI guidelines. All susceptible and nonsusceptible\n                    <jats:italic toggle=\"yes\">C. parapsilosis<\/jats:italic>\n                    isolates were analyzed for ERG11 mutations with Sanger sequencing. T-ARMS-PCR was fully concordant with the Sanger sequencing (100% of sensitivity and specificity) for detection of Y132F mutations. T-ARMS-PCR method could be a rapid, simple, accurate, and economical assay in the early detection of the most common cause of fluconazole resistance in\n                    <jats:italic toggle=\"yes\">C. parapsilosis<\/jats:italic>\n                    isolates. In routine laboratories with high\n                    <jats:italic toggle=\"yes\">C. parapsilosis<\/jats:italic>\n                    isolation rates, performing the T-ARMS-PCR for early detection of the most common reason of fluconazole resistance in\n                    <jats:italic toggle=\"yes\">C. parapsilo<\/jats:italic>\n                    sis, could be a life-saving approach for directing antifungal therapy before obtaining the definitive antifungal susceptibility tests results.\n                  <\/jats:p>","DOI":"10.1089\/mdr.2023.0198","type":"journal-article","created":{"date-parts":[[2024,2,12]],"date-time":"2024-02-12T17:03:15Z","timestamp":1707757395000},"page":"210-213","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":3,"title":["Rapid Tetra-Primer Amplification Refractory Mutation System-Polymerase Chain Reaction Protocol for Detection of Y132F Mutation in Fluconazole Resistant\n                    <i>Candida parapsilosis<\/i>"],"prefix":"10.1177","volume":"30","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9889-8039","authenticated-orcid":false,"given":"Sevgi","family":"\u00d6zt\u00fcrk","sequence":"first","affiliation":[{"name":"Koc University Is Bank Center for Infectious Disease (KUISCID), Istanbul, Turkey."},{"name":"Ko\u00e7 University, Graduate School of Health Science, Medical Microbiology Department, Istanbul, Turkey."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5052-2577","authenticated-orcid":false,"given":"K\u00fcbra","family":"\u00c7am","sequence":"additional","affiliation":[{"name":"Koc University Is Bank Center for Infectious Disease (KUISCID), Istanbul, Turkey."},{"name":"Ko\u00e7 University, Graduate School of Health Science, Medical Microbiology Department, Istanbul, Turkey."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gizem","family":"Babuccu","sequence":"additional","affiliation":[{"name":"Koc University Is Bank Center for Infectious Disease (KUISCID), Istanbul, Turkey."},{"name":"Ko\u00e7 University, Graduate School of Health Science, Medical Microbiology Department, Istanbul, Turkey."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Uzay Altay","family":"Onem","sequence":"additional","affiliation":[{"name":"School of Medicine, Koc University, Istanbul, Turkey."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Serhat","family":"Ayd\u0131n","sequence":"additional","affiliation":[{"name":"School of Medicine, Koc University, Istanbul, Turkey."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mert","family":"Ku\u015fkucu","sequence":"additional","affiliation":[{"name":"Koc University Is Bank Center for Infectious Disease (KUISCID), Istanbul, Turkey."},{"name":"Microbiology Department, Cerrahpasa Faculty of Medicine, Cerrahpasa University, Istanbul, Turkey."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6505-4582","authenticated-orcid":false,"given":"\u00d6zlem","family":"Do\u011fan","sequence":"additional","affiliation":[{"name":"Koc University Is Bank Center for Infectious Disease (KUISCID), Istanbul, Turkey."},{"name":"Microbiology Department, School of Medicine, Koc University, Istanbul, Turkey."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2024,5,23]]},"reference":[{"issue":"6","key":"e_1_3_4_2_2","doi-asserted-by":"crossref","first-page":"5","DOI":"10.1111\/1469-0691.12539","article-title":"Global trends in the distribution of Candida species causing candidemia","volume":"20","author":"Guinea J","year":"2014","unstructured":"Guinea J. Global trends in the distribution of Candida species causing candidemia. Clin Microbiol Infect, 2014; 20 Suppl 6:5\u201310. doi: 10.1111\/1469-0691.12539.","journal-title":"Clin Microbiol Infect"},{"issue":"1","key":"e_1_3_4_3_2","doi-asserted-by":"crossref","first-page":"S79","DOI":"10.1093\/ofid\/ofy358","article-title":"Twenty years of the SENTRY antifungal surveillance program: Results for Candida species from 1997\u20132016","volume":"6","author":"Pfaller MA","year":"2019","unstructured":"Pfaller MA, , Diekema DJ, , Turnidge JD, et al. Twenty years of the SENTRY antifungal surveillance program: Results for Candida species from 1997\u20132016. Open Forum Infect Dis, 2019; 6(Supplement_1):S79\u2013S94; doi: 10.1093\/ofid\/ofy358","journal-title":"Open Forum Infect Dis"},{"issue":"1","key":"e_1_3_4_4_2","doi-asserted-by":"crossref","first-page":"105992","DOI":"10.1016\/j.ijantimicag.2020.105992","article-title":"Effect of initial antifungal therapy on mortality among patients with bloodstream infections with different Candida species and resistance to antifungal agents: A multicentre observational study by the Turkish Fungal Infections Study Group","volume":"56","author":"Ye\u015filkaya A","year":"2020","unstructured":"Do\u011fan \u00d6, Ye\u015filkaya A, Menek\u015fe \u015e, et al. Effect of initial antifungal therapy on mortality among patients with bloodstream infections with different Candida species and resistance to antifungal agents: A multicentre observational study by the Turkish Fungal Infections Study Group. Int J Antimicrob Agents, 2020; 56(1):105992; doi: 10.1016\/j.ijantimicag.2020.105992","journal-title":"Int J Antimicrob Agents"},{"issue":"3","key":"e_1_3_4_5_2","doi-asserted-by":"crossref","first-page":"138","DOI":"10.3390\/jof6030138","article-title":"The quiet and underappreciated rise of drug-resistant invasive fungal pathogens","volume":"6","author":"Arastehfar A","year":"2020","unstructured":"Arastehfar A, , Lass-Fl\u00f6rl C, , Garcia-Rubio R, et al. The quiet and underappreciated rise of drug-resistant invasive fungal pathogens. J Fungi (Basel), 2020; 6(3):138; doi: 10.3390\/jof6030138","journal-title":"J Fungi (Basel)"},{"issue":"9","key":"e_1_3_4_6_2","doi-asserted-by":"crossref","first-page":"1566","DOI":"10.3390\/genes13091566","article-title":"Next generation and other sequencing technologies in diagnostic microbiology and infectious diseases","volume":"13","author":"Hilt EE","year":"2022","unstructured":"Hilt EE, , Ferrieri P. Next generation and other sequencing technologies in diagnostic microbiology and infectious diseases. Genes (Basel), 2022; 13(9):1566; doi: 10.3390\/genes13091566","journal-title":"Genes (Basel)"},{"key":"e_1_3_4_7_2","doi-asserted-by":"crossref","first-page":"952611","DOI":"10.3389\/fmed.2022.952611","article-title":"Appropriate empirical antifungal therapy is associated with a reduced mortality rate in intensive care unit patients with invasive fungal infection: A real-world retrospective study based on the MIMIC-IV database","volume":"9","author":"Zhang MK","year":"2022","unstructured":"Zhang MK, , Rao ZG, , Ma T, et al. Appropriate empirical antifungal therapy is associated with a reduced mortality rate in intensive care unit patients with invasive fungal infection: A real-world retrospective study based on the MIMIC-IV database. 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