{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T17:51:48Z","timestamp":1792000308245,"version":"4.4.0"},"reference-count":21,"publisher":"SAGE Publications","issue":"3","license":[{"start":{"date-parts":[[2024,3,1]],"date-time":"2024-03-01T00:00:00Z","timestamp":1709251200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Microbial Drug Resistance"],"published-print":{"date-parts":[[2024,3]]},"abstract":"<jats:p>\n                    Multidrug-resistant\n                    <jats:italic toggle=\"yes\">Neisseria gonorrhoeae<\/jats:italic>\n                    is a serious concern worldwide. Resistance to \u03b2-lactam antibiotics occurs through mutations in penicillin-binding proteins (PBPs), acquisition of \u03b2-lactamases, and alteration of antibiotic penetration. Mosaic structures of\n                    <jats:italic toggle=\"yes\">penA<\/jats:italic>\n                    , which encodes PBP2, play a major role in resistance to \u03b2-lactams, especially cephalosporins. Ceftriaxone (CRO) is recognized as the only satisfiable antibiotic for the treatment of gonococcal infections; however, CRO-resistant isolates have emerged in the community. Here, we examined the affinity of \u03b2-lactam antibiotics for recombinant PBP2 in a competition assay using fluorescence-labeled penicillin. We found no or little difference in the affinities of penicillins and meropenem (MEM) for PBP2 from cefixime (CFM)-reduced-susceptible strain and cephalosporin-resistant strain. However, the affinity of cephalosporins, including CRO, for PBP2 from the cephalosporin-resistant strain was markedly lower than that for PBP2 from the CFM-reduced-susceptible-resistant strain. Notably, piperacillin (PIP) showed almost the same affinity for PBP2 from penicillin-susceptible, CFM-reduced-susceptible, and cephalosporin (including CRO)-resistant strains. Thus, PIP\/tazobactam and MEM are candidate antibiotics for the treatment of CRO-resistant\/multidrug-resistant\n                    <jats:italic toggle=\"yes\">N. gonorrhoeae<\/jats:italic>\n                    .\n                  <\/jats:p>","DOI":"10.1089\/mdr.2023.0256","type":"journal-article","created":{"date-parts":[[2024,1,12]],"date-time":"2024-01-12T14:35:47Z","timestamp":1705070147000},"page":"141-146","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":4,"title":["Affinity of \u03b2-Lactam Antibiotics for\n                    <i>Neisseria gonorrhoeae<\/i>\n                    Penicillin-Binding Protein 2 Having Wild, Cefixime-Reduced-Susceptible, and Cephalosporin (Ceftriaxone)-Resistant\n                    <i>penA<\/i>\n                    Alleles"],"prefix":"10.1177","volume":"30","author":[{"given":"Yoshiki","family":"Hiyama","sequence":"first","affiliation":[{"name":"Department of Urology, Sapporo Medical University School of Medicine, Sapporo, Japan."},{"name":"Department of Urology, NTT Medical Center Sapporo, Sapporo, Japan."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Soh","family":"Yamamoto","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Sapporo Medical University School of Medicine, Sapporo, Japan."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Toyotaka","family":"Sato","sequence":"additional","affiliation":[{"name":"Laboratory of Veterinary Hygiene, Faculty of Veterinary Medicine, Hokkaido University, Sapporo, Japan."},{"name":"Graduate School of Infectious Diseases, Hokkaido University, Sapporo, Japan."},{"name":"One Health Research Center, Hokkaido University, Sapporo, Japan."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Noriko","family":"Ogasawara","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Sapporo Medical University School of Medicine, Sapporo, Japan."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Naoya","family":"Masumori","sequence":"additional","affiliation":[{"name":"Department of Urology, Sapporo Medical University School of Medicine, Sapporo, Japan."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Satoshi","family":"Takahashi","sequence":"additional","affiliation":[{"name":"Department of Infection Control and Laboratory Medicine, Sapporo Medical University School of Medicine, Sapporo, Japan."},{"name":"Division of Laboratory Medicine, Sapporo Medical University Hospital, Sapporo, Japan."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shin-ichi","family":"Yokota","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Sapporo Medical University School of Medicine, Sapporo, Japan."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2024,3,6]]},"reference":[{"issue":"11","key":"e_1_3_5_2_2","doi-asserted-by":"crossref","first-page":"e627","DOI":"10.1016\/S2666-5247(21)00171-3","article-title":"WHO global antimicrobial resistance surveillance for Neisseria gonorrhoeae 2017\u201318: A retrospective observational study","volume":"2","author":"Unemo M","year":"2021","unstructured":"Unemo M, , Lahra MM, , Escher M, et al. WHO global antimicrobial resistance surveillance for Neisseria gonorrhoeae 2017\u201318: A retrospective observational study. Lancet Microbe, 2021; 2(11):e627\u2013e636; doi: 10.1016\/S2666-5247(21)00171-3","journal-title":"Lancet Microbe"},{"issue":"4","key":"e_1_3_5_3_2","doi-asserted-by":"crossref","DOI":"10.1128\/JCM.01461-19","article-title":"laboratory capacity for Neisseria gonorrhoeae Antimicrobial susceptibility testing and whole-genome sequencing through the CDC's Antibiotic Resistance Laboratory Network","volume":"58","author":"Kersh EN","year":"2020","unstructured":"Kersh EN, , Pham CD, , Papp JR, et al. Expanding U.S. laboratory capacity for Neisseria gonorrhoeae Antimicrobial susceptibility testing and whole-genome sequencing through the CDC's Antibiotic Resistance Laboratory Network. J Clin Microbiol, 2020; 58(4); doi: 10.1128\/JCM.01461-19","journal-title":"J Clin Microbiol"},{"issue":"3","key":"e_1_3_5_4_2","doi-asserted-by":"crossref","first-page":"149","DOI":"10.1097\/OLQ.0000000000000556","article-title":"Antimicrobial susceptibility of Neisseria gonorrhoeae in Japan from 2000 to 2015","volume":"44","author":"Yasuda M","year":"2017","unstructured":"Yasuda M, , Hatazaki K, , Ito S, et al. Antimicrobial susceptibility of Neisseria gonorrhoeae in Japan from 2000 to 2015. Sex Transm Dis, 2017; 44(3):149\u2013153; doi: 10.1097\/OLQ.0000000000000556","journal-title":"Sex Transm Dis"},{"key":"e_1_3_5_5_2","doi-asserted-by":"publisher","DOI":"10.1093\/clinids\/20.Supplement_1.S47"},{"key":"e_1_3_5_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jiac.2019.12.001"},{"key":"e_1_3_5_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00325-11"},{"key":"e_1_3_5_8_2","doi-asserted-by":"crossref","first-page":"896607","DOI":"10.3389\/fmicb.2022.896607","article-title":"In vitro susceptibility to \u03b2-lactam antibiotics and viability of Neisseria gonorrhoeae strains producing plasmid-mediated broad- and extended-spectrum \u03b2-lactamases","volume":"13","author":"Kandinov I","year":"2022","unstructured":"Kandinov I, , Gryadunov D, , Vinokurova A, et al. In vitro susceptibility to \u03b2-lactam antibiotics and viability of Neisseria gonorrhoeae strains producing plasmid-mediated broad- and extended-spectrum \u03b2-lactamases. Front Microbiol, 2022; 13:896607; doi: 10.3389\/fmicb.2022.896607","journal-title":"Front Microbiol"},{"key":"e_1_3_5_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00010-14"},{"issue":"4","key":"e_1_3_5_10_2","doi-asserted-by":"crossref","first-page":"571","DOI":"10.1007\/s10156-013-0637-2","article-title":"Nationwide surveillance of the antimicrobial susceptibility of Neisseria gonorrhoeae from male urethritis in Japan","volume":"19","author":"Hamasuna R","year":"2013","unstructured":"Hamasuna R, , Yasuda M, , Ishikawa K, et al. Nationwide surveillance of the antimicrobial susceptibility of Neisseria gonorrhoeae from male urethritis in Japan. 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J Infect Chemother, 2015; 21(5):340\u2013345; doi: 10.1016\/j.jiac.2015.01.010","journal-title":"J Infect Chemother"},{"key":"e_1_3_5_12_2","doi-asserted-by":"publisher","DOI":"10.1089\/mdr.2018.0263"},{"issue":"7","key":"e_1_3_5_13_2","doi-asserted-by":"crossref","first-page":"1769","DOI":"10.1093\/jac\/dkab104","article-title":"Molecular characterization of Neisseria gonorrhoeae isolates collected through a national surveillance programme in Japan, 2013: Evidence of the emergence of a ceftriaxone-resistant strain from a ceftriaxone-susceptible lineage","volume":"76","author":"Hanao M","year":"2021","unstructured":"Hanao M, , Aoki K, , Ishii Y, et al. Molecular characterization of Neisseria gonorrhoeae isolates collected through a national surveillance programme in Japan, 2013: Evidence of the emergence of a ceftriaxone-resistant strain from a ceftriaxone-susceptible lineage. J Antimicrob Chemother, 2021; 76(7):1769\u20131775; doi: 10.1093\/jac\/dkab104","journal-title":"J Antimicrob Chemother"},{"issue":"7","key":"e_1_3_5_14_2","doi-asserted-by":"crossref","first-page":"3029","DOI":"10.1128\/AAC.00093-13","article-title":"Identification of amino acids conferring high-level resistance to expanded-spectrum cephalosporins in the penA gene from Neisseria gonorrhoeae strain H041","volume":"57","author":"Tomberg J","year":"2013","unstructured":"Tomberg J, , Unemo M, , Ohnishi M, et al. Identification of amino acids conferring high-level resistance to expanded-spectrum cephalosporins in the penA gene from Neisseria gonorrhoeae strain H041. 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Intensive Care Med, 1994; 20(Suppl. 3):S14\u2013S20; doi: 10.1007\/BF01745246","journal-title":"Intensive Care Med"}],"container-title":["Microbial Drug Resistance"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/full-xml\/10.1089\/mdr.2023.0256","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1089\/mdr.2023.0256","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1089\/mdr.2023.0256","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T20:41:21Z","timestamp":1777668081000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/full\/10.1089\/mdr.2023.0256"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,3]]},"references-count":21,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2024,3]]}},"alternative-id":["10.1089\/mdr.2023.0256"],"URL":"https:\/\/doi.org\/10.1089\/mdr.2023.0256","relation":{},"ISSN":["1076-6294","1931-8448"],"issn-type":[{"value":"1076-6294","type":"print"},{"value":"1931-8448","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,3]]}}}