{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,4]],"date-time":"2026-09-04T01:09:34Z","timestamp":1788484174486,"version":"build-2803163510"},"reference-count":40,"publisher":"SAGE Publications","issue":"5","license":[{"start":{"date-parts":[[2024,5,1]],"date-time":"2024-05-01T00:00:00Z","timestamp":1714521600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Microbial Drug Resistance"],"published-print":{"date-parts":[[2024,5]]},"abstract":"<jats:p>\n                    <jats:italic toggle=\"yes\">Klebsiella pneumoniae<\/jats:italic>\n                    stands out as a major opportunistic pathogen responsible for both hospital- and community-acquired bacterial infections. This study comprehensively assesses the antibiotic resistance, amikacin persistent patterns, and biofilm-forming ability of 247 isolates of\n                    <jats:italic toggle=\"yes\">K. pneumoniae<\/jats:italic>\n                    obtained from an intensive care unit of a tertiary hospital in Vietnam. Microdilution assays, conducted on a 96-well plate, determined the minimum inhibitory concentrations (MICs) of amikacin. Susceptibility data for other antibiotics were gathered from the antibiogram profile. Stationary-phase bacteria were exposed to 50\u2009\u00d7\u2009MIC, and viable bacteria counts were measured to determine amikacin persistence. Biofilm forming capacity on 96-well polystyrene surfaces was assessed by biomass and viable bacteria. The prevalence of resistance was notably high across most antibiotics, with 64.8% classified as carbapenem-resistant\n                    <jats:italic toggle=\"yes\">K. pneumoniae<\/jats:italic>\n                    and 81.4% as multidrug resistant. Amikacin, however, exhibited a relatively low rate of resistance. Of the isolates, 58.2% demonstrated a moderate to strong biofilm formation capacity, and these were found to be poorly responsive to amikacin.\n                    <jats:italic toggle=\"yes\">K. pneumoniae<\/jats:italic>\n                    reveals a significant inclination for amikacin persistence, with \u223c45% of isolates displaying an antibiotic antibiotic-survival ratio exceeding 10%. The study sheds light on challenges in treating of\n                    <jats:italic toggle=\"yes\">K. pneumoniae<\/jats:italic>\n                    infection in Vietnam, encompassing a high prevalence of antibiotic resistance, a substantial ability to form biofilm, and a notable rate of antibiotic persistence.\n                  <\/jats:p>","DOI":"10.1089\/mdr.2023.0267","type":"journal-article","created":{"date-parts":[[2024,3,21]],"date-time":"2024-03-21T11:12:02Z","timestamp":1711019522000},"page":"203-209","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":5,"title":["Antibiotic Resistance, Biofilm Formation, and Persistent Phenotype of\n                    <i>Klebsiella pneumoniae<\/i>\n                    in a Vietnamese Tertiary Hospital: A Focus on Amikacin"],"prefix":"10.1177","volume":"30","author":[{"given":"Hong Nhung","family":"Pham","sequence":"first","affiliation":[{"name":"Department Microbiology, Hanoi Medical University, Hanoi, Vietnam."},{"name":"Department of Microbiology, Bach Mai Hospital, Hanoi, Vietnam."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thi Dung Nhi","family":"Than","sequence":"additional","affiliation":[{"name":"Department Microbiology, Hanoi Medical University, Hanoi, Vietnam."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hoang Anh","family":"Nguyen","sequence":"additional","affiliation":[{"name":"National Drug Information and Adverse Drug Reactions Monitoring Centre, Hanoi University of Pharmacy, Hanoi, Vietnam."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dinh Hoa","family":"Vu","sequence":"additional","affiliation":[{"name":"National Drug Information and Adverse Drug Reactions Monitoring Centre, Hanoi University of Pharmacy, Hanoi, Vietnam."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thanh Huong","family":"Phung","sequence":"additional","affiliation":[{"name":"Faculty of Biotechnology, Hanoi University of Pharmacy, Hanoi, Vietnam."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-2610-8420","authenticated-orcid":false,"given":"Tiep Khac","family":"Nguyen","sequence":"additional","affiliation":[{"name":"Faculty of Biotechnology, Hanoi University of Pharmacy, Hanoi, Vietnam."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2024,5,23]]},"reference":[{"issue":"2","key":"e_1_3_5_2_2","first-page":"234","article-title":"Carbapenem-resistant Klebsiella pneumoniae: Virulence factors, molecular epidemiology and latest updates in treatment options","volume":"12","author":"Karampatakis T","year":"2023","unstructured":"Karampatakis T, , Tsergouli K, , Behzadi P. Carbapenem-resistant Klebsiella pneumoniae: Virulence factors, molecular epidemiology and latest updates in treatment options. Antibiot Basel Switz, 2023; 12(2):234; doi: 10.3390\/antibiotics12020234","journal-title":"Antibiot Basel Switz"},{"key":"e_1_3_5_3_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2021.750662"},{"issue":"4","key":"e_1_3_5_4_2","doi-asserted-by":"crossref","first-page":"e255","DOI":"10.1016\/S2666-5247(22)00338-X","article-title":"Evidence of widespread endemic populations of highly multidrug resistant Klebsiella pneumoniae in hospital settings in Hanoi, Vietnam: A prospective cohort study","volume":"4","author":"Pham MH","year":"2023","unstructured":"Pham MH, , Hoi LT, , Beale MA, et al. Evidence of widespread endemic populations of highly multidrug resistant Klebsiella pneumoniae in hospital settings in Hanoi, Vietnam: A prospective cohort study. Lancet Microbe, 2023; 4(4):e255\u2013e263; doi: 10.1016\/S2666-5247(22)00338-X","journal-title":"Lancet Microbe"},{"issue":"11","key":"e_1_3_5_5_2","doi-asserted-by":"crossref","first-page":"e0032122","DOI":"10.1128\/aac.00321-22","article-title":"Optimizing meropenem in highly resistant Klebsiella pneumoniae environments: Population pharmacokinetics and dosing simulations in critically Ill patients","volume":"66","author":"Truong AQ","year":"2022","unstructured":"Truong AQ, , Dao XC, , Vu DH, et al. Optimizing meropenem in highly resistant Klebsiella pneumoniae environments: Population pharmacokinetics and dosing simulations in critically Ill patients. 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