{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T19:33:29Z","timestamp":1777923209944,"version":"3.51.4"},"reference-count":25,"publisher":"SAGE Publications","issue":"6","license":[{"start":{"date-parts":[[2024,6,1]],"date-time":"2024-06-01T00:00:00Z","timestamp":1717200000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Microbial Drug Resistance"],"published-print":{"date-parts":[[2024,6]]},"abstract":"\n This study examines the genetic contexts and evolutionary steps responsible for the formation of the widely spread transposon Tn\n 6925<\/jats:italic>\n carrying\n bla<\/jats:italic>\n TEM<\/jats:sub>\n and\n aacC2e,<\/jats:italic>\n which confers resistance to beta-lactam and aminoglycoside antibiotics in Gram-negative bacteria. The\n bla<\/jats:italic>\n TEM-1<\/jats:sub>\n and\n aacC2e<\/jats:italic>\n genes were found in several transposons. They were first observed within an IS\n 26<\/jats:italic>\n bounded 3.7\u2009kb transposon (Tn\n 6925)<\/jats:italic>\n on several\n Acinetobacter baumannii<\/jats:italic>\n plasmids located within a 4.7\u2009kb\n dif<\/jats:italic>\n module. Truncated and expanded variations of Tn\n 6925<\/jats:italic>\n were found across other\n A. baumannii<\/jats:italic>\n plasmids, as well as in other Gram-negative bacteria (including\n Vibrio cholerae<\/jats:italic>\n ). Moreover,\n bla<\/jats:italic>\n TEM-1<\/jats:sub>\n and\n aacC2e<\/jats:italic>\n were in much larger resistance-heavy transposons including the ISAba1-bounded 24.6\u2009kb (here called Tn\n 6927<\/jats:italic>\n ), found in an\n A. baumannii<\/jats:italic>\n chromosome. A novel ISKpn12-bounded transposon was also observed to contain\n bla<\/jats:italic>\n TEM<\/jats:sub>\n and\n aacC2e<\/jats:italic>\n which was found interrupting Tn\n 5393<\/jats:italic>\n along with an IS\n 26<\/jats:italic>\n pseudo-compound transposon to form a 24.9\u2009kb resistance island in an\n Acinetobacter pittii<\/jats:italic>\n plasmid. Multiple mobile genetic elements are involved in the formation of transposon structures that circulate\n bla<\/jats:italic>\n TEM<\/jats:sub>\n and\n aacC2e<\/jats:italic>\n . Among these, IS\n 26<\/jats:italic>\n and ISAba1 appear to have played a major role in the formation and spread of these elements in the\n Acinetobacter<\/jats:italic>\n species.\n <\/jats:p>","DOI":"10.1089\/mdr.2023.0299","type":"journal-article","created":{"date-parts":[[2024,4,9]],"date-time":"2024-04-09T17:01:30Z","timestamp":1712682090000},"page":"273-278","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":2,"title":["Transposons Carrying the\n aacC2e<\/i>\n Aminoglycoside and\n bla<\/i>\n TEM<\/sub>\n Beta-Lactam Resistance Genes in\n Acinetobacter<\/i>"],"prefix":"10.1177","volume":"30","author":[{"given":"Liam A.","family":"Tobin","sequence":"first","affiliation":[{"name":"Australian Institute for Microbiology and Infection, University of Technology Sydney, Ultimo, Australia."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Amy K.","family":"Cain","sequence":"additional","affiliation":[{"name":"ARC Centre of Excellence in Synthetic Biology, School of Natural Sciences, Macquarie University, Sydney, Australia."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Steven P.","family":"Djordjevic","sequence":"additional","affiliation":[{"name":"Australian Institute for Microbiology and Infection, University of Technology Sydney, Ultimo, Australia."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3614-7261","authenticated-orcid":false,"given":"Mehrad","family":"Hamidian","sequence":"additional","affiliation":[{"name":"Australian Institute for Microbiology and Infection, University of Technology Sydney, Ultimo, Australia."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2024,6,14]]},"reference":[{"issue":"8","key":"e_1_3_3_2_2","doi-asserted-by":"crossref","first-page":"e1011520","DOI":"10.1371\/journal.ppat.1011520","article-title":"Portrait of a killer: Uncovering resistance mechanisms and global spread of Acinetobacter baumannii","volume":"19","author":"Cain AK","year":"2023","unstructured":"Cain AK, , Hamidian M. 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