{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T16:52:13Z","timestamp":1790009533379,"version":"4.0.1"},"reference-count":29,"publisher":"SAGE Publications","issue":"10","license":[{"start":{"date-parts":[[2024,10,1]],"date-time":"2024-10-01T00:00:00Z","timestamp":1727740800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2024,10,1]],"date-time":"2024-10-01T00:00:00Z","timestamp":1727740800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Microbial Drug Resistance"],"published-print":{"date-parts":[[2024,10,1]]},"abstract":"<jats:sec>\n                    <jats:title>Objective:<\/jats:title>\n                    <jats:p>\n                      Characterization of the multidrug resistance (MDR) region in\n                      <jats:italic toggle=\"yes\">P. aeruginosa<\/jats:italic>\n                      strain PA59 revealed the presence of antibiotic resistance genes, including\n                      <jats:italic toggle=\"yes\">bla<\/jats:italic>\n                      <jats:sub>IMP-45<\/jats:sub>\n                      and\n                      <jats:italic toggle=\"yes\">bla<\/jats:italic>\n                      <jats:sub>VIM-2<\/jats:sub>\n                      , within a complex genetic landscape of mobile genetic elements.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods:<\/jats:title>\n                    <jats:p>\n                      Carbapenem-resistant\n                      <jats:italic toggle=\"yes\">Pseudomonas aeruginosa<\/jats:italic>\n                      (CRPA) strains were isolated from Shanghai Changhai Hospital. Polymerase chain reaction (PCR) was used to detect the\n                      <jats:italic toggle=\"yes\">\u03b2<\/jats:italic>\n                      -lactamase genes in the isolated strains. Strains carrying two or more genes were subjected to whole-genome sequencing (WGS) and in-depth bioinformatics analysis.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results:<\/jats:title>\n                    <jats:p>\n                      A total of 94 CRPA strains were isolated, among which PA59 was determined to carry\n                      <jats:italic toggle=\"yes\">bla<\/jats:italic>\n                      <jats:sub>IMP-45<\/jats:sub>\n                      and\n                      <jats:italic toggle=\"yes\">bla<\/jats:italic>\n                      <jats:sub>VIM-2<\/jats:sub>\n                      genes. Compared with single-gene positive or other\n                      <jats:italic toggle=\"yes\">bla<\/jats:italic>\n                      <jats:sub>IMP<\/jats:sub>\n                      and\n                      <jats:italic toggle=\"yes\">bla<\/jats:italic>\n                      <jats:sub>VIM<\/jats:sub>\n                      dual-gene positive strains reported, PA59 exhibited a broader range of drug resistance. We discovered a multidrug resistant (MDR)\u2013related region composed of various mobile elements in the PA59 chromosome. This region carried many resistance genes, including the target genes\n                      <jats:italic toggle=\"yes\">bla<\/jats:italic>\n                      <jats:sub>IMP-45<\/jats:sub>\n                      and\n                      <jats:italic toggle=\"yes\">bla<\/jats:italic>\n                      <jats:sub>VIM-2<\/jats:sub>\n                      . By further comparing the mobile elements GI13 and Ph08, we speculated that this integron structure carrying\n                      <jats:italic toggle=\"yes\">bla<\/jats:italic>\n                      <jats:sub>IMP-45<\/jats:sub>\n                      and\n                      <jats:italic toggle=\"yes\">bla<\/jats:italic>\n                      <jats:sub>VIM-2<\/jats:sub>\n                      was initially integrated into the genomic island or prophage, forming a more complex genetic structure, and then further integrated into the PA59 chromosome through plasmids. Phylogenetic tree analysis showed limited sequence similarity between PA59 and other CRPA strains.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions:<\/jats:title>\n                    <jats:p>\n                      This study identified PA59 as the first reported\n                      <jats:italic toggle=\"yes\">P. aeruginosa<\/jats:italic>\n                      strain carrying both\n                      <jats:italic toggle=\"yes\">bla<\/jats:italic>\n                      <jats:sub>IMP-45<\/jats:sub>\n                      and\n                      <jats:italic toggle=\"yes\">bla<\/jats:italic>\n                      <jats:sub>VIM-2<\/jats:sub>\n                      on the chromosome. The assembly and annotation of the PA59 genome provide valuable insights into the genomic diversity and gene content of this clinically important pathogen, aiding the development of effective strategies against antibiotic resistance.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1089\/mdr.2024.0059","type":"journal-article","created":{"date-parts":[[2024,9,5]],"date-time":"2024-09-05T05:23:00Z","timestamp":1725513780000},"page":"422-431","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":9,"title":["Characterization of the Chromosomally Located Metallo-\n                    <i>\u03b2<\/i>\n                    -Lactamase Genes\n                    <i>bla<\/i>\n                    <sub>IMP-45<\/sub>\n                    and\n                    <i>bla<\/i>\n                    <sub>VIM-2<\/sub>\n                    in a Carbapenem-Resistant\n                    <i>Pseudomonas aeruginosa<\/i>\n                    Clinical Isolate"],"prefix":"10.1177","volume":"30","author":[{"given":"Wei","family":"Ma","sequence":"first","affiliation":[{"name":"Department of Laboratory Diagnostics, Changhai Hospital, Naval Medical University, Shanghai, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jie","family":"Guo","sequence":"additional","affiliation":[{"name":"Department of Laboratory Diagnostics, Changhai Hospital, Naval Medical University, Shanghai, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Changzi","family":"Deng","sequence":"additional","affiliation":[{"name":"Department of Laboratory Diagnostics, Changhai Hospital, Naval Medical University, Shanghai, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaochun","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Laboratory Diagnostics, Changhai Hospital, Naval Medical University, Shanghai, China."},{"name":"Department of Clinical Laboratory, The Second Affiliated Hospital of Soochow University, Suzhou, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yukai","family":"Sun","sequence":"additional","affiliation":[{"name":"Department of Laboratory Diagnostics, Changhai Hospital, Naval Medical University, Shanghai, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li","family":"Xu","sequence":"additional","affiliation":[{"name":"Department of Laboratory Diagnostics, Changhai Hospital, Naval Medical University, Shanghai, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qin","family":"Qin","sequence":"additional","affiliation":[{"name":"Department of Laboratory Diagnostics, Changhai Hospital, Naval Medical University, Shanghai, China."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2024,10,4]]},"reference":[{"key":"e_1_3_5_2_2","doi-asserted-by":"publisher","DOI":"10.1080\/22221751.2022.2048972"},{"key":"e_1_3_5_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmi.2021.11.012"},{"key":"e_1_3_5_4_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749-6632.2012.06796.x"},{"key":"e_1_3_5_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jgar.2020.10.016"},{"key":"e_1_3_5_6_2","doi-asserted-by":"publisher","DOI":"10.3947\/ic.2015.47.2.81"},{"key":"e_1_3_5_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/spectrum.02035-22"},{"key":"e_1_3_5_8_2","doi-asserted-by":"publisher","DOI":"10.1590\/0037-8682-0044-2018"},{"key":"e_1_3_5_9_2","doi-asserted-by":"publisher","DOI":"10.1099\/jmm.0.081802-0"},{"key":"e_1_3_5_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jgar.2021.08.009"},{"issue":"10","key":"e_1_3_5_11_2","first-page":"1373","article-title":"Epidemiology and high incidence of metallo-\u03b2-lactamase and AmpC-\u03b2-lactamases in nosocomial Pseudomonas aeruginosa","volume":"24","author":"Muddassir M","year":"2021","unstructured":"Muddassir M, , Munir S, , Raza A, et al. 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