{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T10:38:23Z","timestamp":1791887903193,"version":"4.3.4"},"reference-count":33,"publisher":"SAGE Publications","issue":"2","license":[{"start":{"date-parts":[[2025,2,1]],"date-time":"2025-02-01T00:00:00Z","timestamp":1738368000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Microbial Drug Resistance"],"published-print":{"date-parts":[[2025,2]]},"abstract":"<jats:p>\n                    Carbapenem and colistin-resistant\n                    <jats:italic toggle=\"yes\">Klebsiella pneumoniae<\/jats:italic>\n                    pose a significant threat to public health, particularly in intensive care units, due to high morbidity and mortality rates. This study aimed to analyze five NDM carbapenemase-producing multidrug-resistant\n                    <jats:italic toggle=\"yes\">K. pneumoniae<\/jats:italic>\n                    isolates from different hospitals. Antimicrobial susceptibility testing, hypermucoviscosity analysis, biofilm production assessment, MLST, PCR, and whole-genome sequencing were conducted. All isolates harbored NDM-5 metallo-\u03b2-lactamase, belonging to MLST 307, were biofilm producers and exhibited a stop codon (Q30) along MgrB. Genomic analysis revealed multiple-replicon plasmids carrying resistance genes, notably\n                    <jats:italic toggle=\"yes\">bla<\/jats:italic>\n                    <jats:sub>NDM-5<\/jats:sub>\n                    ,\n                    <jats:italic toggle=\"yes\">bla<\/jats:italic>\n                    <jats:sub>CTX-M-15<\/jats:sub>\n                    ,\n                    <jats:italic toggle=\"yes\">rmtB<\/jats:italic>\n                    , and\n                    <jats:italic toggle=\"yes\">qnrB1<\/jats:italic>\n                    , with complex genetic structures encoding several mobile genetic elements, including the Tn3 family and IS26. All isolates harbored\n                    <jats:italic toggle=\"yes\">wzi<\/jats:italic>\n                    173 (capsule-locus KL102),\n                    <jats:italic toggle=\"yes\">iutA<\/jats:italic>\n                    (a siderophore-associated gene), and the type 3 fimbriae\n                    <jats:italic toggle=\"yes\">mrkABCDFHIJ<\/jats:italic>\n                    operon. The core genome single nucleotide polymorphisms (SNPs) analysis suggests the circulation of two strains of ST307 clone (SNPs range differences 4\u201377). These findings highlight the potential plasticity of the high-risk ST307 clone and the urgent need for surveillance and intervention strategies to combat antimicrobial resistance. To our knowledge, this is the first report of\n                    <jats:italic toggle=\"yes\">K. pneumoniae<\/jats:italic>\n                    ST307 carrying\n                    <jats:italic toggle=\"yes\">bla<\/jats:italic>\n                    <jats:sub>NDM-5<\/jats:sub>\n                    and the first description of ST307 in Uruguay. The presence of\n                    <jats:italic toggle=\"yes\">bla<\/jats:italic>\n                    <jats:sub>NDM-5<\/jats:sub>\n                    and pan-aminoglycoside resistance\n                    <jats:italic toggle=\"yes\">rmtB<\/jats:italic>\n                    genes are identified for the first time in Uruguay.\n                  <\/jats:p>","DOI":"10.1089\/mdr.2024.0065","type":"journal-article","created":{"date-parts":[[2025,2,5]],"date-time":"2025-02-05T23:26:01Z","timestamp":1738797961000},"page":"42-51","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":3,"title":["Emergence of Multidrug-Resistant NDM-5-Producing ST307\n                    <i>Klebsiella pneumoniae<\/i>\n                    in Uruguay, 2023"],"prefix":"10.1177","volume":"31","author":[{"given":"Carmen","family":"Magallanes","sequence":"first","affiliation":[{"name":"Laboratorio de Microbiolog\u00eda Cl\u00ednica, Departamento de Bioqu\u00edmica Cl\u00ednica, Facultad de Qu\u00edmica, Universidad de la Rep\u00fablica, Montevideo, Uruguay."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eliana","family":"Eugster","sequence":"additional","affiliation":[{"name":"Laboratorio de Microbiolog\u00eda Cl\u00ednica, Departamento de Bioqu\u00edmica Cl\u00ednica, Facultad de Qu\u00edmica, Universidad de la Rep\u00fablica, Montevideo, Uruguay."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Felipe","family":"Clavijo","sequence":"additional","affiliation":[{"name":"Laboratorio de Microbiolog\u00eda Molecular, Departamento de Biociencias, Facultad de Qu\u00edmica, Universidad de la Rep\u00fablica, Montevideo, Uruguay."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mar\u00eda In\u00e9s","family":"Siri","sequence":"additional","affiliation":[{"name":"Laboratorio de Microbiolog\u00eda Molecular, Departamento de Biociencias, Facultad de Qu\u00edmica, Universidad de la Rep\u00fablica, Montevideo, Uruguay."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jorge","family":"Cantero","sequence":"additional","affiliation":[{"name":"Departamento DETEMA, Facultad de Qu\u00edmica, Universidad de la Rep\u00fablica, Montevideo, Uruguay."},{"name":"Centro de Investigaciones M\u00e9dicas, Facultad de Ciencias de la Salud, Universidad Nacional del Este, Minga Guaz\u00fa, Paraguay."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patricia","family":"Echeverr\u00eda","sequence":"additional","affiliation":[{"name":"Sociedad M\u00e9dica Universal, Montevideo, Uruguay."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Josefina","family":"Torello","sequence":"additional","affiliation":[{"name":"Laboratorio Central del HCFFAA, Montevideo, Uruguay."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mercedes","family":"Castro","sequence":"additional","affiliation":[{"name":"Laboratorio de Microbiolog\u00eda Cl\u00ednica, Departamento de Bioqu\u00edmica Cl\u00ednica, Facultad de Qu\u00edmica, Universidad de la Rep\u00fablica, Montevideo, Uruguay."},{"name":"Sociedad M\u00e9dica Universal, Montevideo, Uruguay."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carolina","family":"M\u00e1rquez","sequence":"additional","affiliation":[{"name":"Laboratorio de Microbiolog\u00eda Cl\u00ednica, Departamento de Bioqu\u00edmica Cl\u00ednica, Facultad de Qu\u00edmica, Universidad de la Rep\u00fablica, Montevideo, Uruguay."},{"name":"Instituto de Qu\u00edmica Biol\u00f3gica, Facultad de Ciencias, Universidad de la Rep\u00fablica, Montevideo, Uruguay."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2025,2,12]]},"reference":[{"key":"e_1_3_7_2_2","doi-asserted-by":"publisher","DOI":"10.1097\/SMJ.0b013e3181fd7d5a"},{"issue":"12","key":"e_1_3_7_3_2","doi-asserted-by":"crossref","first-page":"944","DOI":"10.1016\/j.tim.2016.09.007","article-title":"Klebsiella pneumoniae population genomics and antimicrobial-resistant clones","volume":"24","author":"Wyres KL","year":"2016","unstructured":"Wyres KL, , Holt KE. 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