{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T19:33:43Z","timestamp":1777923223226,"version":"3.51.4"},"reference-count":40,"publisher":"SAGE Publications","issue":"10","license":[{"start":{"date-parts":[[2024,10,1]],"date-time":"2024-10-01T00:00:00Z","timestamp":1727740800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Microbial Drug Resistance"],"published-print":{"date-parts":[[2024,10,1]]},"abstract":"<jats:p>\n                    Carbapenem resistance in\n                    <jats:italic toggle=\"yes\">Acinetobacter baumannii<\/jats:italic>\n                    is a critical global health threat attributed to transferrable carbapenemase genes. Carbapenemase genotyping using polymerase chain reaction (PCR) presents a challenge in resource-limited settings because of its technical requirements. This study designed new loop-mediated isothermal amplification (LAMP) primers using multiple sequence alignment-based workflows, validated the primer performance against multiple target variants\n                    <jats:italic toggle=\"yes\">in silico<\/jats:italic>\n                    , and developed novel LAMP assays (LAntRN-OXA23 and LAntRN-ISAba1) to detect the transferable\n                    <jats:italic toggle=\"yes\">bla<\/jats:italic>\n                    <jats:sub>OXA-23-like<\/jats:sub>\n                    carbapenemase genes and\n                    <jats:italic toggle=\"yes\">ISAba1<\/jats:italic>\n                    elements in pure cultures and\n                    <jats:italic toggle=\"yes\">A. baumannii<\/jats:italic>\n                    -spiked serum samples. The designed LAMP primers bind to the conserved regions of their highly polymorphic targets, with their\n                    <jats:italic toggle=\"yes\">in silico<\/jats:italic>\n                    performance comparable with other published primers. The\n                    <jats:italic toggle=\"yes\">in vitro<\/jats:italic>\n                    LAMP assays (using 30 PCR-profiled\n                    <jats:italic toggle=\"yes\">A. baumannii<\/jats:italic>\n                    and 10 standard multidrug-resistant gram-negative isolates) have 100% concordance with the PCR-positive clinical samples, limits of detection as low as 1 pg\/\u00b5L (200 copies\/\u00b5L), and specificities of 57.89\u2013100%. Both assays produced positive results when testing DNA samples (extracted using a commercial kit) from\n                    <jats:italic toggle=\"yes\">bla<\/jats:italic>\n                    <jats:sub>OXA-23-like<\/jats:sub>\n                    and\n                    <jats:italic toggle=\"yes\">ISAba1-bla<\/jats:italic>\n                    <jats:sub>OXA-51-like<\/jats:sub>\n                    PCR-positive\n                    <jats:italic toggle=\"yes\">A. baumannii<\/jats:italic>\n                    -spiked normal human sera (five set-ups per target). In summary, the LAMP assays accurately detected the target genes and have applications in infection management, control, and point-of-care testing in resource-limited healthcare settings.\n                  <\/jats:p>","DOI":"10.1089\/mdr.2024.0075","type":"journal-article","created":{"date-parts":[[2024,8,28]],"date-time":"2024-08-28T04:16:14Z","timestamp":1724818574000},"page":"432-441","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":2,"title":["Colorimetric Loop-Mediated Isothermal Amplification Assays Accurately Detect\n                    <i>bla<\/i>\n                    <sub>OXA-23-like<\/sub>\n                    and\n                    <i>ISAba1<\/i>\n                    Genes from\n                    <i>Acinetobacter baumannii<\/i>\n                    in Pure Cultures and Spiked Human Sera"],"prefix":"10.1177","volume":"30","author":[{"given":"Mark B.","family":"Carascal","sequence":"first","affiliation":[{"name":"Pathogen-Host-Environment Interactions Research Laboratory, Institute of Biology, College of Science, University of the Philippines, Diliman, Quezon City, Philippines."},{"name":"Clinical and Translational Research Institute, The Medical City, Pasig City, Philippines."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Raul V.","family":"Destura","sequence":"additional","affiliation":[{"name":"Clinical and Translational Research Institute, The Medical City, Pasig City, Philippines."},{"name":"National Training Center for Biosafety and Biosecurity, National Institutes of Health, University of the Philippines, Manila, Philippines."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4659-6132","authenticated-orcid":false,"given":"Windell L.","family":"Rivera","sequence":"additional","affiliation":[{"name":"Pathogen-Host-Environment Interactions Research Laboratory, Institute of Biology, College of Science, University of the Philippines, Diliman, Quezon City, Philippines."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2024,10,4]]},"reference":[{"key":"e_1_3_6_2_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2022.1045206"},{"key":"e_1_3_6_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/aac.01975-21"},{"key":"e_1_3_6_4_2","doi-asserted-by":"publisher","DOI":"10.2147\/IDR.S277364"},{"issue":"10","key":"e_1_3_6_5_2","first-page":"e000306","article-title":"Emergence, molecular mechanisms and global spread of carbapenem-resistant Acinetobacter baumannii","volume":"5","author":"Hamidian M","year":"2019","unstructured":"Hamidian M, , Nigro SJ. 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