{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T19:33:33Z","timestamp":1777923213178,"version":"3.51.4"},"reference-count":16,"publisher":"SAGE Publications","issue":"1","license":[{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Microbial Drug Resistance"],"published-print":{"date-parts":[[2025,1]]},"abstract":"<jats:p>\n                    Carbapenenemase producers, particularly the metallo-\u03b2-lactamase (MBL) types in\n                    <jats:italic toggle=\"yes\">Pseudomonas aeruginosa<\/jats:italic>\n                    , have emerged as an urgent threat in health care settings. MBLs require zinc at their catalytic site and can be inhibited by dimercaptosuccinic acid (DMSA), a metal chelator known for the treatment of lead and mercury intoxication. Isogenic strains of wild-type and OprD-deleted\n                    <jats:italic toggle=\"yes\">P. aeruginosa<\/jats:italic>\n                    PA14, were constructed, producing the MBLs VIM-2, NDM-1, SPM-1, IMP-1, and AIM-1, or the non-MBL carbapenemases, GES-5 and KPC-2. In addition, 59 previously characterized clinical isolates of\n                    <jats:italic toggle=\"yes\">P. aeruginosa<\/jats:italic>\n                    producing different \u00df-lactamases (including carbapenemases), and with known outer-membrane porin OprD status, were utilized. Minimal inhibitory concentrations values of imipenem and meropenem, and DMSA combinations were determined, and time-kill assays were performed with PA14 expressing VIM-2. Results indicated a significant additive effect of DMSA (most effective at 3 mM) and carbapenems in recombinant and clinical strains of\n                    <jats:italic toggle=\"yes\">P. aeruginosa<\/jats:italic>\n                    expressing MBLs, in particular against VIM producers, which are the most prevalent carbapenemases in\n                    <jats:italic toggle=\"yes\">P. aeruginosa<\/jats:italic>\n                    . This effect was best evidenced with meropenem and in strains without OprD modification. DMSA shows promising efficacy, particularly in combination therapy with meropenem, for treating infections caused by MBL-producing\n                    <jats:italic toggle=\"yes\">P. aeruginosa<\/jats:italic>\n                    .\n                  <\/jats:p>","DOI":"10.1089\/mdr.2024.0104","type":"journal-article","created":{"date-parts":[[2024,11,25]],"date-time":"2024-11-25T03:35:44Z","timestamp":1732505744000},"page":"16-20","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":2,"title":["<i>In-Vitro<\/i>\n                    Activity of Dimercaptosuccinic Acid in Combination with Carbapenems Against Carbapenem-Resistant\n                    <i>Pseudomonas aeruginosa<\/i>"],"prefix":"10.1177","volume":"31","author":[{"given":"Maxime","family":"Bouvier","sequence":"first","affiliation":[{"name":"Medical and Molecular Microbiology, Faculty of Science and Medicine, University of Fribourg, Fribourg, Switzerland."},{"name":"Swiss National Reference Center for Emerging Antibiotic Resistance (NARA), University of Fribourg, Fribourg, Switzerland."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Samanta","family":"Freire","sequence":"additional","affiliation":[{"name":"Medical and Molecular Microbiology, Faculty of Science and Medicine, University of Fribourg, Fribourg, Switzerland."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jacqueline","family":"Findlay","sequence":"additional","affiliation":[{"name":"Medical and Molecular Microbiology, Faculty of Science and Medicine, University of Fribourg, Fribourg, Switzerland."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Patrice","family":"Nordmann","sequence":"additional","affiliation":[{"name":"Medical and Molecular Microbiology, Faculty of Science and Medicine, University of Fribourg, Fribourg, Switzerland."},{"name":"Institute for Microbiology, University of Lausanne and University Hospital Centre, Lausanne, Switzerland."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2025,1,8]]},"reference":[{"key":"e_1_3_5_2_2","unstructured":"World Health Organization (WHO). Global priority list of antibiotic-resistant bacteria to guide research discovery and development of new antibiotics. 2017. Available from: http:\/\/remed.org\/wp-content\/uploads\/2017\/03\/lobal-priority-list-of-antibiotic-resistant-bacteria-2017.pdf [Last accessed: March28 2024]"},{"issue":"7","key":"e_1_3_5_3_2","doi-asserted-by":"crossref","first-page":"S521","DOI":"10.1093\/cid\/ciz824","article-title":"Epidemiology and diagnostics of carbapenem resistance in Gram-negative Bacteria","volume":"69","author":"Nordmann P","year":"2019","unstructured":"Nordmann P, , Poirel L. Epidemiology and diagnostics of carbapenem resistance in Gram-negative Bacteria. Clin Infect Dis., 2019; 69(Suppl 7):S521\u2013S528; doi: 10.1093\/cid\/ciz824","journal-title":"Clin Infect Dis."},{"issue":"1","key":"e_1_3_5_4_2","doi-asserted-by":"crossref","first-page":"350","DOI":"10.1016\/j.drudis.2018.07.003","article-title":"Updates on the pathogenicity status of Pseudomonas aeruginosa","volume":"24","author":"Azam MW","year":"2019","unstructured":"Azam MW, , Khan AU. Updates on the pathogenicity status of Pseudomonas aeruginosa. Drug Discov Today., 2019; 24(1):350\u2013359; doi: 10.1016\/j.drudis.2018.07.003","journal-title":"Drug Discov Today."},{"issue":"2","key":"e_1_3_5_5_2","doi-asserted-by":"crossref","first-page":"81","DOI":"10.3947\/ic.2015.47.2.81","article-title":"Epidemiology and characteristics of metallo-\u03b2-lactamase-producing Pseudomonas aeruginosa","volume":"47","author":"Hong DJ","year":"2015","unstructured":"Hong DJ, , Bae IK, , Jang IH, et al. Epidemiology and characteristics of metallo-\u03b2-lactamase-producing Pseudomonas aeruginosa. Infect Chemother., 2015; 47(2):81\u201397; doi: 10.3947\/ic.2015.47.2.81","journal-title":"Infect Chemother."},{"issue":"4","key":"e_1_3_5_6_2","first-page":"507","article-title":"The intriguing carbapenemases of Pseudomonas aeruginosa: Current status, genetic profile, and global epidemiology","volume":"95","author":"Hammoudi Halat D","year":"2022","unstructured":"Hammoudi Halat D, , Ayoub Moubareck C. The intriguing carbapenemases of Pseudomonas aeruginosa: Current status, genetic profile, and global epidemiology. Yale J Biol Med., 2022; 95(4):507\u2013515.","journal-title":"Yale J Biol Med."},{"key":"e_1_3_5_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00031-19"},{"key":"e_1_3_5_8_2","doi-asserted-by":"crossref","first-page":"1060736","DOI":"10.3389\/fgene.2022.1060736","article-title":"Biochemical exploration of \u03b2-lactamase inhibitors","volume":"13","author":"Arer V","year":"2022","unstructured":"Arer V, , Kar D. Biochemical exploration of \u03b2-lactamase inhibitors. Front Genet, 2022; 13:1060736; doi: 10.3389\/fgene.2022.1060736","journal-title":"Front Genet"},{"issue":"9","key":"e_1_3_5_9_2","doi-asserted-by":"crossref","first-page":"2382","DOI":"10.1093\/jac\/dkad210","article-title":"Wide dissemination of Gram-negative bacteria producing the taniborbactam-resistant NDM-9 variant: A one health concern","volume":"78","author":"Le Terrier C","year":"2023","unstructured":"Le Terrier C, , Nordmann P, , Buchs C, et al. Wide dissemination of Gram-negative bacteria producing the taniborbactam-resistant NDM-9 variant: A one health concern. J Antimicrob Chemother., 2023; 78(9):2382\u20132384; doi: 10.1093\/jac\/dkad210","journal-title":"J Antimicrob Chemother."},{"key":"e_1_3_5_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejmech.2022.114677"},{"key":"e_1_3_5_11_2","doi-asserted-by":"publisher","DOI":"10.1093\/qjmed\/hcp114"},{"issue":"1","key":"e_1_3_5_12_2","doi-asserted-by":"crossref","first-page":"11","DOI":"10.2174\/1389557043487583","article-title":"Chemical and biological considerations in the treatment of metal intoxications by chelating agents","volume":"4","author":"Andersen O","year":"2004","unstructured":"Andersen O. Chemical and biological considerations in the treatment of metal intoxications by chelating agents. Mini Rev Med Chem., 2004; 4(1):11\u201321; doi: 10.2174\/1389557043487583","journal-title":"Mini Rev Med Chem."},{"issue":"12","key":"e_1_3_5_13_2","doi-asserted-by":"crossref","first-page":"3593","DOI":"10.1093\/jac\/dkaa347","article-title":"Dimercaptosuccinic acid in combination with carbapenems against isogenic strains of Escherichia coli producing or not producing a metallo-\u03b2-lactamase in vitro and in murine peritonitis","volume":"75","author":"Cheminet G","year":"2020","unstructured":"Cheminet G, , de Lastours V, , Poirel L, et al. Dimercaptosuccinic acid in combination with carbapenems against isogenic strains of Escherichia coli producing or not producing a metallo-\u03b2-lactamase in vitro and in murine peritonitis. J Antimicrob Chemother., 2020; 75(12):3593\u20133600; doi: 10.1093\/jac\/dkaa347","journal-title":"J Antimicrob Chemother."},{"key":"e_1_3_5_14_2","doi-asserted-by":"publisher","DOI":"10.1093\/femsre\/fux020"},{"issue":"31","key":"e_1_3_5_15_2","doi-asserted-by":"crossref","first-page":"e2107644118","DOI":"10.1073\/pnas.2107644118","article-title":"Outer membrane permeability: Antimicrobials and diverse nutrients bypass porins in Pseudomonas aeruginosa","volume":"118","author":"Ude J","year":"2021","unstructured":"Ude J, , Tripathi V, , Buyck JM, et al. Outer membrane permeability: Antimicrobials and diverse nutrients bypass porins in Pseudomonas aeruginosa. Proc Natl Acad Sci U S A., 2021; 118(31):e2107644118; doi: 10.1073\/pnas.2107644118","journal-title":"Proc Natl Acad Sci U S A."},{"key":"e_1_3_5_16_2","volume-title":"Performance Standards for Antimicrobial Susceptibility Testing","author":"Clinical and Laboratory Standards Institute","year":"2023","unstructured":"Clinical and Laboratory Standards Institute. Performance Standards for Antimicrobial Susceptibility Testing, 33rd ed. M100. Clinical and Laboratory Standards Institute: Wayne, PA; 2023.","edition":"33"},{"issue":"2","key":"e_1_3_5_17_2","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1016\/j.ijmm.2011.10.001","article-title":"Structure and function of OprD protein in Pseudomonas aeruginosa: From antibiotic resistance to novel therapies","volume":"302","author":"Li H","year":"2012","unstructured":"Li H, , Luo YF, , Williams BJ, et al. Structure and function of OprD protein in Pseudomonas aeruginosa: From antibiotic resistance to novel therapies. Int J Med Microbiol., 2012; 302(2):63\u201368; doi: 10.1016\/j.ijmm.2011.10.001","journal-title":"Int J Med Microbiol."}],"container-title":["Microbial Drug Resistance"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/full-xml\/10.1089\/mdr.2024.0104","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1089\/mdr.2024.0104","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1089\/mdr.2024.0104","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T20:41:42Z","timestamp":1777668102000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/full\/10.1089\/mdr.2024.0104"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,1]]},"references-count":16,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2025,1]]}},"alternative-id":["10.1089\/mdr.2024.0104"],"URL":"https:\/\/doi.org\/10.1089\/mdr.2024.0104","relation":{},"ISSN":["1076-6294","1931-8448"],"issn-type":[{"value":"1076-6294","type":"print"},{"value":"1931-8448","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,1]]}}}