{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T19:45:05Z","timestamp":1777923905693,"version":"3.51.4"},"reference-count":38,"publisher":"SAGE Publications","issue":"4","license":[{"start":{"date-parts":[[2011,8,1]],"date-time":"2011-08-01T00:00:00Z","timestamp":1312156800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Nucleic Acid Therapeutics"],"published-print":{"date-parts":[[2011,8]]},"abstract":"<jats:p>\n                    Patients with sickle cell disease (SCD) often suffer painful vaso-occlusive episodes caused in part by the adhesion of sickle erythrocytes (SS-RBC) to the vascular endothelium. To investigate inhibition of SS-RBC adhesion as a possible treatment for vaso-occlusion, 2 adhesion molecules, \u03b1\n                    <jats:sub>v<\/jats:sub>\n                    \u03b2\n                    <jats:sub>3<\/jats:sub>\n                    and P-selectin, were targeted by high-affinity RNA aptamers. An\n                    <jats:italic toggle=\"yes\">in vitro<\/jats:italic>\n                    flow chamber assay was used to test the antiadhesion activity of \u03b1\n                    <jats:sub>v<\/jats:sub>\n                    \u03b2\n                    <jats:sub>3<\/jats:sub>\n                    aptamer clone 17.16. Human SS-RBC were passed across a confluent monolayer of thrombin-stimulated human umbilical vein endothelial cells (HUVEC) at a constant rate. \u03b1\n                    <jats:sub>v<\/jats:sub>\n                    \u03b2\n                    <jats:sub>3<\/jats:sub>\n                    aptamer reduced SS-RBC adhesion to activated endothelial cells to the level seen with untreated HUVEC. An aptamer reactive with complement component 8 was used as a negative control and exerted no inhibition, confirming the specificity of \u03b1\n                    <jats:sub>v<\/jats:sub>\n                    \u03b2\n                    <jats:sub>3<\/jats:sub>\n                    aptamer (\n                    <jats:italic toggle=\"yes\">P<\/jats:italic>\n                    =0.04). At 2\u2009dyn\/cm\n                    <jats:sup>2<\/jats:sup>\n                    shear stress, 30\u2009nM \u03b1\n                    <jats:sub>v<\/jats:sub>\n                    \u03b2\n                    <jats:sub>3<\/jats:sub>\n                    aptamer showed maximal effect in decreasing SS-RBC adhesion to HUVEC. The antiadhesive activity of the P-selectin aptamer clone PF377 was also tested using HUVEC pretreated with IL-13 to upregulate expression of P-selectin as seen in activated endothelial cells. At 1\u2009dyn\/cm\n                    <jats:sup>2<\/jats:sup>\n                    shear stress, 60\u2009nM of P-selectin aptamer had antiadhesion activity similar to heparin, a known inhibitor of SS-RBC adhesion to P-selectin. A negative control did not prevent adhesion (\n                    <jats:italic toggle=\"yes\">P<\/jats:italic>\n                    =0.05). These data show the potential utility of aptamers to block endothelial adhesion molecules to prevent or treat vaso-occlusion in SCD.\n                  <\/jats:p>","DOI":"10.1089\/nat.2010.0270","type":"journal-article","created":{"date-parts":[[2011,7,28]],"date-time":"2011-07-28T05:11:08Z","timestamp":1311829868000},"page":"275-283","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":15,"title":["RNA Aptamer Therapy for Vaso-Occlusion in Sickle Cell Disease"],"prefix":"10.1177","volume":"21","author":[{"given":"Angela D.","family":"Burnette","sequence":"first","affiliation":[{"name":"Duke University Medical Center"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shahid M.","family":"Nimjee","sequence":"additional","affiliation":[{"name":"Duke University Medical Center"},{"name":"Duke University Medical Center"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Milena","family":"Batchvarova","sequence":"additional","affiliation":[{"name":"Duke University Medical Center"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rahima","family":"Zennadi","sequence":"additional","affiliation":[{"name":"Duke University Medical Center"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marilyn J.","family":"Telen","sequence":"additional","affiliation":[{"name":"Duke University Medical Center"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jun-Ichi","family":"Nishimura","sequence":"additional","affiliation":[{"name":"Osaka University Graduate School of Medicine","place":["Japan"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bruce A.","family":"Sullenger","sequence":"additional","affiliation":[{"name":"Duke University Medical Center"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2011,8]]},"reference":[{"key":"e_1_3_3_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0162-3109(99)00020-X"},{"key":"e_1_3_3_3_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V81.8.2137.2137"},{"key":"e_1_3_3_4_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI10817"},{"key":"e_1_3_3_5_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.143.7.2081"},{"key":"e_1_3_3_6_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V97.11.3401"},{"key":"e_1_3_3_7_1","doi-asserted-by":"publisher","DOI":"10.1055\/s-0037-1616241"},{"key":"e_1_3_3_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/0891-5849(93)90004-E"},{"key":"e_1_3_3_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/346818a0"},{"key":"e_1_3_3_10_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2004-02-0713"},{"key":"e_1_3_3_11_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.01054.2006"},{"key":"e_1_3_3_12_1","doi-asserted-by":"publisher","DOI":"10.1097\/00062752-200203000-00003"},{"key":"e_1_3_3_13_1","doi-asserted-by":"publisher","DOI":"10.1037\/0278-6133.23.3.267"},{"key":"e_1_3_3_14_1","first-page":"236","article-title":"Prostaglandin E2 induces expression of P-selectin (CD62P) on cultured human umbilical vein endothelial cells and enhances endothelial binding of CD4-T-cells","volume":"70","author":"HAILER N.P.","year":"2000","unstructured":"HAILER N.P., , OPPERMANN E., , LECKEL K., , CINATL J., , MARKUS B.H., , BLAHET A.R.A. 2000. 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