{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,13]],"date-time":"2026-05-13T07:12:31Z","timestamp":1778656351497,"version":"3.51.4"},"reference-count":19,"publisher":"SAGE Publications","issue":"4","license":[{"start":{"date-parts":[[2012,7,25]],"date-time":"2012-07-25T00:00:00Z","timestamp":1343174400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Nucleic Acid Therapeutics"],"published-print":{"date-parts":[[2012,8,1]]},"abstract":"<jats:p>MicroRNAs (miRNAs) might behave as tumor suppressors and for that they are under consideration as novel therapeutic drugs. We tested the tumor suppressor activity of miRNA-34a (miR-34a) by measuring cell proliferation of the follicular lymphoma cell line DoHH2 transfected with this miRNA. We report that miR-34a did not inhibit cell proliferation notwithstanding a marked down-regulation of c-MYC. Interestingly, DoHH2 transfected cells showed a significant p53 down-regulation, suggesting that c-MYC positively controls p53 and the failed inhibition of cell proliferation is probably due to the down-regulation of the c-MYC\/p53 axis. In keeping with this, c-MYC silencing also down-regulated p53 and had no effect on cell proliferation. In accordance with this hypothesis, etoposide or nutlin-3 treatment or a small interfering RNA (siRNA) against BCL6 (B-cell lymphoma 6) inhibited the proliferation of DoHH2 cells by up-regulating p53 without affecting either miR-34a or c-MYC levels. These results indicate that the proliferation is controlled by the regulatory axis c-MYC\/p53 and suggest that paradoxically miR-34a behaves as a pro-proliferative rather than an anti-proliferative miRNA in DoHH2 cells.<\/jats:p>","DOI":"10.1089\/nat.2012.0343","type":"journal-article","created":{"date-parts":[[2018,8,9]],"date-time":"2018-08-09T22:54:02Z","timestamp":1533855242000},"page":"283-288","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":9,"title":["The Over-Expression of miR-34a Fails to Block DoHH2 Lymphoma Cell Proliferation by Reducing p53 via c-MYC Down-Regulation"],"prefix":"10.1177","volume":"22","author":[{"given":"Milena","family":"Rizzo","sequence":"first","affiliation":[{"name":"Institute of Clinical Physiology","place":["Italy"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Laura","family":"Mariani","sequence":"additional","affiliation":[{"name":"Institute of Clinical Physiology","place":["Italy"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sara","family":"Cavallini","sequence":"additional","affiliation":[{"name":"Institute of Clinical Physiology","place":["Italy"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marcella","family":"Simili","sequence":"additional","affiliation":[{"name":"Institute of Clinical Physiology","place":["Italy"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Giuseppe","family":"Rainaldi","sequence":"additional","affiliation":[{"name":"Institute of Clinical Physiology","place":["Italy"]},{"name":"Istituto Toscano Tumori","place":["Italy"]}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2012,7,25]]},"reference":[{"key":"e_1_3_3_2_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-09-2598"},{"key":"e_1_3_3_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/cdd.2009.109"},{"key":"e_1_3_3_4_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0511155103"},{"key":"e_1_3_3_5_1","first-page":"1198","article-title":"Concurrent activation of MYC and BCL2 in B cell non-Hodgkin lymphoma cell lines by translocation of both oncogenes to the same immunoglobulin heavy chain locus","volume":"10","author":"DYER M.J.","year":"1996","unstructured":"DYER M.J., , LILLINGTON D.M., , BASTARD C., , TILLY H., , LENS D., , HEWARD J.M., , STRANKS G., , MORILLA R., , MONRAD S., , GUGLIELMI P. et al. 1996. Concurrent activation of MYC and BCL2 in B cell non-Hodgkin lymphoma cell lines by translocation of both oncogenes to the same immunoglobulin heavy chain locus. 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