{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T19:42:25Z","timestamp":1777923745125,"version":"3.51.4"},"reference-count":24,"publisher":"SAGE Publications","issue":"5","license":[{"start":{"date-parts":[[2013,8,24]],"date-time":"2013-08-24T00:00:00Z","timestamp":1377302400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Nucleic Acid Therapeutics"],"published-print":{"date-parts":[[2013,10,1]]},"abstract":"<jats:p>One of the hallmarks of progression of influenza virus replication is the step involving the virus uncoating that occurs in the host cytoplasm. The BM2 ion channel protein of influenza B virus is highly conserved and is essentially required during the uncoating processes of virus, thus an attractive target for designing antiviral drugs. We screened several DNA enzymes (Dzs) containing the 10-23 catalytic motif against the influenza B virus BM2 RNA. Dzs directed against the predicted single-stranded bulge regions showed sequence-specific cleavage activities. The Dz209 not only showed significant intracellular reduction of BM2 gene expression in transient-expression system but also provided considerable protection against influenza B virus challenge in MDCK cells. Our findings suggest that the Dz molecule can be used as selective and effective inhibitor of viral RNA replication, and can be explored further for development of a potent therapeutic agent against influenza B virus infection.<\/jats:p>","DOI":"10.1089\/nat.2013.0432","type":"journal-article","created":{"date-parts":[[2013,8,24]],"date-time":"2013-08-24T10:00:49Z","timestamp":1377338449000},"page":"355-362","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":11,"title":["Sequence-Specific Cleavage of BM2 Gene Transcript of Influenza B Virus by 10-23 Catalytic Motif Containing DNA Enzymes Significantly Inhibits Viral RNA Translation and Replication"],"prefix":"10.1177","volume":"23","author":[{"given":"Binod","family":"Kumar","sequence":"first","affiliation":[{"name":"University of Delhi","place":["India"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Prashant","family":"Kumar","sequence":"additional","affiliation":[{"name":"University of Delhi","place":["India"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Roopali","family":"Rajput","sequence":"additional","affiliation":[{"name":"University of Delhi","place":["India"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Latika","family":"Saxena","sequence":"additional","affiliation":[{"name":"University of Delhi","place":["India"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mradul K.","family":"Daga","sequence":"additional","affiliation":[{"name":"Maulana Azad Medical College and Lok Nayak Hospital","place":["India"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Madhu","family":"Khanna","sequence":"additional","affiliation":[{"name":"University of Delhi","place":["India"]}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2013,8,24]]},"reference":[{"key":"e_1_3_3_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.mt.6300231"},{"key":"e_1_3_3_3_1","doi-asserted-by":"publisher","DOI":"10.1089\/154545704322988049"},{"key":"e_1_3_3_4_1","first-page":"97","article-title":"Recombination and complementation between orthomyxoviruses under conditions of abortive infection","volume":"23","author":"GHENKINA D.B.","year":"1979","unstructured":"GHENKINA D.B., , GHENDON Y.Z. 1979. Recombination and complementation between orthomyxoviruses under conditions of abortive infection. Acta Virol., 23:97\u2013106.","journal-title":"Acta Virol."},{"key":"e_1_3_3_5_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj3530701"},{"key":"e_1_3_3_6_1","doi-asserted-by":"publisher","DOI":"10.1089\/oli.2004.14.249"},{"key":"e_1_3_3_7_1","first-page":"396","article-title":"[Inhibition of hepatitis B virus S gene and C gene expression by different 10-23 DNAzymes substrate-recognition domains.]","volume":"45","author":"HOU W.","year":"2006","unstructured":"HOU W., , WO J.E., , LIU K., , LI M.W., , CHEN L.W., , HU Z.R, , LIU R.H., , HU M.J. 2006. [Inhibition of hepatitis B virus S gene and C gene expression by different 10-23 DNAzymes substrate-recognition domains.] Zhonghua Nei Ke Za Zhi., 45:396\u2013399.","journal-title":"Zhonghua Nei Ke Za Zhi."},{"key":"e_1_3_3_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/0166-0934(80)90020-8"},{"key":"e_1_3_3_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1742-4658.2005.04986.x"},{"key":"e_1_3_3_10_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12038-008-0066-z"},{"key":"e_1_3_3_11_1","first-page":"144","article-title":"Quantification of viral load in clinical specimens collected from different body sites of patients infected with influenza viruses","volume":"3","author":"KUMAR B.","year":"2011","unstructured":"KUMAR B., , KHANNA M., , KUMAR P., , GUPTA A., , DAGA M.K., , SARKAR M.C., , CHADHA M.S., , MISHRA A.C., , KAUR H. 2011. Quantification of viral load in clinical specimens collected from different body sites of patients infected with influenza viruses. Int. J. Med. Med. Sci., 3:144\u2013148.","journal-title":"Int. J. Med. Med. Sci."},{"key":"e_1_3_3_12_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12033-011-9437-z"},{"key":"e_1_3_3_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.antiviral.2010.05.001"},{"key":"e_1_3_3_14_1","unstructured":"LAMB R.A. KRUG R.M. 2001. Orthomyxoviridae: the viruses and their replication. In: Fields virology. 4th Knipe DM Howley PM. Philadelphia: Lippincott Williams & Wilkins 1487\u2013531."},{"key":"e_1_3_3_15_1","doi-asserted-by":"publisher","DOI":"10.1099\/0022-1317-80-10-2573"},{"key":"e_1_3_3_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0168-8278(99)80341-9"},{"key":"e_1_3_3_17_1","doi-asserted-by":"publisher","DOI":"10.1089\/nat.2012.0359"},{"key":"e_1_3_3_18_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.94.9.4262"},{"key":"e_1_3_3_19_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi9812221"},{"key":"e_1_3_3_20_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.051013898"},{"key":"e_1_3_3_21_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12038-012-9216-4"},{"key":"e_1_3_3_22_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gng038"},{"key":"e_1_3_3_23_1","doi-asserted-by":"publisher","DOI":"10.1089\/oli.2007.9998"},{"key":"e_1_3_3_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/0042-6822(91)90075-M"},{"key":"e_1_3_3_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-5793(04)00073-0"}],"container-title":["Nucleic Acid Therapeutics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/full-xml\/10.1089\/nat.2013.0432","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1089\/nat.2013.0432","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1089\/nat.2013.0432","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T20:58:44Z","timestamp":1777669124000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1089\/nat.2013.0432"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,8,24]]},"references-count":24,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2013,10,1]]}},"alternative-id":["10.1089\/nat.2013.0432"],"URL":"https:\/\/doi.org\/10.1089\/nat.2013.0432","relation":{},"ISSN":["2159-3337","2159-3345"],"issn-type":[{"value":"2159-3337","type":"print"},{"value":"2159-3345","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,8,24]]}}}