{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T20:25:39Z","timestamp":1777926339499,"version":"3.51.4"},"reference-count":25,"publisher":"SAGE Publications","issue":"1","license":[{"start":{"date-parts":[[2010,1,1]],"date-time":"2010-01-01T00:00:00Z","timestamp":1262304000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Neurotrauma"],"published-print":{"date-parts":[[2010,1]]},"abstract":"<jats:p>\n                    The efficacy of Schwann-cell cultivation can be enhanced by\n                    <jats:italic toggle=\"yes\">in vitro<\/jats:italic>\n                    predegeneration of the harvested cells compared to immediate culture. The aim of this study was to improve Schwann-cell culture efficacy by comparing three different durations of predegeneration.\n                  <\/jats:p>\n                  <jats:p>\n                    The sciatic and median nerves of 6\u20138-week-old Lewis rats were harvested and subjected to either 2-day, 7-day, or 14-day predegeneration in Dulbecco's Modified Eagle's Medium supplemented with 10% fetal calf serum and 1% Penicillin\/Streptomycin. Afterward, tissue was enzymatically dissociated and placed in a modified melanocyte growth medium. The cell count was determined immediately after dissociation while the cell purity was determined one subculture\/trypsinization cycle later after cell attachment to the culture plate by means of optical microscopy and immunocytochemistry. Particular attention was then paid to the Schwann-cell-to-fibroblast relation. The cumulative cell count in the culture was 5.8\u2009\u00d7\u200910\n                    <jats:sup>5<\/jats:sup>\n                    for 2-day, 1.12\u2009\u00d7\u200910\n                    <jats:sup>6<\/jats:sup>\n                    for 7-day, and 1.48\u2009\u00d7\u200910\n                    <jats:sup>6<\/jats:sup>\n                    for 14-day predegeneration. The culture purity was approximately equal for 2- and 7-day predegeneration (88% Schwann cells, 12% fibroblasts after 2 days; 85% Schwann cells, 15% fibroblasts after 7 days). After 14 days, however, cell cultures were significantly debased by fibroblast proliferation (57% Schwann cells, 43% fibroblasts).\n                    <jats:italic toggle=\"yes\">In vitro<\/jats:italic>\n                    predegeneration is a particularly suitable procedural method to increase the cultural Schwann-cell yield. The number of cultivated rat Schwann cells is doubled by 7-day\n                    <jats:italic toggle=\"yes\">in vitro<\/jats:italic>\n                    predegeneration in comparison to 2-day predegeneration. After 14-day predegeneration, however, the culture is significantly debased by fibroblasts. Therefore, 7-day\n                    <jats:italic toggle=\"yes\">in vitro<\/jats:italic>\n                    predegeneration is an advisable predegeneration period.\n                  <\/jats:p>","DOI":"10.1089\/neu.2009.0995","type":"journal-article","created":{"date-parts":[[2009,8,27]],"date-time":"2009-08-27T11:23:59Z","timestamp":1251372239000},"page":"197-203","source":"Crossref","is-referenced-by-count":14,"title":["Efficacy of Various Durations of\n                    <i>In Vitro<\/i>\n                    Predegeneration on the Cell Count and Purity of Rat Schwann-Cell Cultures"],"prefix":"10.1177","volume":"27","author":[{"given":"Armin","family":"Kraus","sequence":"first","affiliation":[{"name":"Eberhard Karls University","place":["Germany"]},{"name":"Eberhard Karls University","place":["Germany"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joachim","family":"T\u00e4ger","sequence":"additional","affiliation":[{"name":"Eberhard Karls University","place":["Germany"]},{"name":"Eberhard Karls University","place":["Germany"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Konrad","family":"Kohler","sequence":"additional","affiliation":[{"name":"Eberhard Karls University","place":["Germany"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Theodora","family":"Manoli","sequence":"additional","affiliation":[{"name":"Eberhard Karls University","place":["Germany"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Max","family":"Haerle","sequence":"additional","affiliation":[{"name":"Orthopaedic Hospital Markgroeningen","place":["Germany"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Frank","family":"Werdin","sequence":"additional","affiliation":[{"name":"Eberhard Karls University","place":["Germany"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J\u00fcrgen","family":"Hoffmann","sequence":"additional","affiliation":[{"name":"University Hospital","place":["Germany"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hans-Eberhard","family":"Schaller","sequence":"additional","affiliation":[{"name":"Eberhard Karls University","place":["Germany"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nektarios","family":"Sinis","sequence":"additional","affiliation":[{"name":"Eberhard Karls University","place":["Germany"]},{"name":"Eberhard Karls University","place":["Germany"]}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2010,1,1]]},"reference":[{"key":"e_1_3_3_2_1","doi-asserted-by":"publisher","DOI":"10.1179\/016164104225013798"},{"key":"e_1_3_3_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1460-9568.1995.tb00682.x"},{"key":"e_1_3_3_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1460-9568.1995.tb00683.x"},{"key":"e_1_3_3_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0142-9612(01)00190-9"},{"key":"e_1_3_3_6_1","doi-asserted-by":"publisher","DOI":"10.1006\/mcne.2000.0859"},{"key":"e_1_3_3_7_1","doi-asserted-by":"publisher","DOI":"10.3171\/jns.2006.104.5.778"},{"key":"e_1_3_3_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0165-0270(99)00034-5"},{"key":"e_1_3_3_9_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.184.6.2311"},{"key":"e_1_3_3_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/jnr.20166"},{"key":"e_1_3_3_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0094-1298(20)31826-5"},{"key":"e_1_3_3_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijom.2006.06.005"},{"key":"e_1_3_3_13_1","doi-asserted-by":"publisher","DOI":"10.1097\/00005373-199807000-00025"},{"key":"e_1_3_3_14_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.172.6.1749"},{"key":"e_1_3_3_15_1","doi-asserted-by":"publisher","DOI":"10.1088\/1741-2560\/1\/3\/004"},{"key":"e_1_3_3_16_1","first-page":"143","article-title":"The degeneration and re-innervation of grafted nerves","volume":"76","author":"Sanders F.K.","year":"1942","unstructured":"Sanders F.K., , Young J.Z. 1942. 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