{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T20:39:50Z","timestamp":1777927190199,"version":"3.51.4"},"reference-count":63,"publisher":"SAGE Publications","issue":"6","license":[{"start":{"date-parts":[[2021,2,26]],"date-time":"2021-02-26T00:00:00Z","timestamp":1614297600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Journal of Neurotrauma"],"published-print":{"date-parts":[[2021,3]]},"abstract":"<jats:p>\n                    Spinal cord injury (SCI) leads to extensive bone loss and high incidence of low-energy fractures. Pulsed electromagnetic fields (PEMF) treatment, as a non-invasive biophysical technique, has proven to be efficient in promoting osteogenesis. The potential osteoprotective effect and mechanism of PEMF on SCI-related bone deterioration, however, remain unknown. The spinal cord of rats was transected at vertebral level T12 to induce SCI. Thirty rats were assigned to the control, SCI, and SCI+PEMF groups (\n                    <jats:italic toggle=\"yes\">n<\/jats:italic>\n                    \u2009=\u200910). One week after surgery, the SCI+PEMF rats were subjected to PEMF (2.0 mT, 15\u2009Hz, 2\u2009h\/day) for eight weeks. Micro-computed tomography results showed that PEMF significantly ameliorated trabecular and cortical bone microarchitecture deterioration induced by SCI. Three-point bending and nanoindentation assays revealed that PEMF significantly improved bone mechanical properties in SCI rats. Serum biomarker and bone histomorphometric analyses demonstrated that PEMF enhanced bone formation, as evidenced by significant increase in serum osteocalcin and P1NP, mineral apposition rate, and osteoblast number on bone surface. The PEMF had no impact, however, on serum bone-resorbing cytokines (TRACP 5b and CTX-1) or osteoclast number on bone surface. The PEMF also attenuated SCI-induced negative changes in osteocyte morphology and osteocyte survival. Moreover, PEMF significantly increased skeletal expression of canonical Wnt ligands (Wnt1 and Wnt10b) and stimulated their downstream p-GSK3\u03b2 and \u03b2-catenin expression in SCI rats. This study demonstrates that PEMF can mitigate the detrimental consequence of SCI on bone quantity\/quality, which might be associated with canonical Wnt signaling-mediated bone formation, and reveals that PEMF may be a promising biophysical approach for resisting osteopenia\/osteoporosis after SCI in clinics.\n                  <\/jats:p>","DOI":"10.1089\/neu.2020.7296","type":"journal-article","created":{"date-parts":[[2020,10,28]],"date-time":"2020-10-28T02:27:40Z","timestamp":1603852060000},"page":"765-776","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":11,"title":["Pulsed Electromagnetic Fields Ameliorate Skeletal Deterioration in Bone Mass, Microarchitecture, and Strength by Enhancing Canonical Wnt Signaling-Mediated Bone Formation in Rats with Spinal Cord Injury"],"prefix":"10.1177","volume":"38","author":[{"given":"Xi","family":"Shao","sequence":"first","affiliation":[{"name":"Department of Biomedical Engineering, Fourth Military Medical University, Xi'an, China."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zedong","family":"Yan","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering, Fourth Military Medical University, Xi'an, China."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dan","family":"Wang","sequence":"additional","affiliation":[{"name":"Lab of Tissue Engineering, Faculty of Life Sciences, Northwest University, Xi'an, China."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yongqing","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering, Fourth Military Medical University, Xi'an, China."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuanjun","family":"Ding","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering, Fourth Military Medical University, Xi'an, China."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Erping","family":"Luo","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering, Fourth Military Medical University, Xi'an, China."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Da","family":"Jing","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering, Fourth Military Medical University, Xi'an, China."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jing","family":"Cai","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering, Fourth Military Medical University, Xi'an, China."},{"name":"College of Basic Medicine, Shaanxi University of Chinese Medicine, Xianyang, China."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2021,2,26]]},"reference":[{"key":"e_1_3_4_2_2","unstructured":"World Health Organization and International Spinal Cord Society (2013). 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