{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T20:41:15Z","timestamp":1777927275322,"version":"3.51.4"},"reference-count":40,"publisher":"SAGE Publications","issue":"7-8","license":[{"start":{"date-parts":[[2022,3,23]],"date-time":"2022-03-23T00:00:00Z","timestamp":1647993600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Journal of Neurotrauma"],"published-print":{"date-parts":[[2022,4]]},"abstract":"<jats:p>\n                    RNA-binding motif 5 (RBM5) is a pro-death tumor suppressor gene in cancer cells. It remains to be determined if it is neurotoxic in the brain or rather if it plays a fundamentally different role in the central nervous system (CNS). Brain-specific RBM5 knockout (KO) mice were given a controlled cortical impact (CCI) traumatic brain injury (TBI). Markers of acute cellular damage and repair were measured in hippocampal homogenates 48\u2009h post-CCI. Hippocampal CA1\/CA3 cell counts were assessed 7 days post-CCI to determine if early changes in injury markers were associated with histological outcome. No genotype-dependent differences were found in the levels of apoptotic markers (caspase 3, caspase 6, and caspase 9). However, KO females had a paradoxical increase in markers of pro-death calpain activation (145\/150-spectrin and breakdown products [SBDP]) and in DNA repair\/survival markers. (pH2A.x and pCREB). CCI-injured male KOs had a significant increase in phosphorylated calcium\/calmodulin-dependent protein kinase II (pCaMKII). Despite sex\/genotype-dependent differences in KOs in the levels of acute cell signaling targets involved in cell death pathways, 7 day hippocampal neuronal survival did not differ from that of wild types (WTs). Similarly, no differences in astrogliosis were observed. Finally, gene analysis revealed increased estrogen receptor \u03b1 (ER\u03b1) levels in the KO hippocampus in females and may suggest a novel mechanism to explain sex-dimorphic effects on cell signaling. In summary, RBM5 inhibition did not affect hippocampal survival after a TBI\n                    <jats:italic toggle=\"yes\">in vivo<\/jats:italic>\n                    but did modify targets involved in neural signal transduction\/Ca\n                    <jats:sup>2+<\/jats:sup>\n                    signaling pathways. Findings here support the view that RBM5 may serve a purpose in the CNS that is dissimilar from its traditional pro-death role in cancer.\n                  <\/jats:p>","DOI":"10.1089\/neu.2021.0362","type":"journal-article","created":{"date-parts":[[2022,2,12]],"date-time":"2022-02-12T07:30:08Z","timestamp":1644651008000},"page":"577-589","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":3,"title":["RNA Binding Motif 5 Gene Deletion Modulates Cell Signaling in a Sex-Dependent Manner but Not Hippocampal Cell Death"],"prefix":"10.1177","volume":"39","author":[{"given":"Jeffrey","family":"Farooq","sequence":"first","affiliation":[{"name":"USF Health Heart Institute, University of South Florida, Morsani College of Medicine, Tampa, Florida, USA."},{"name":"Department of Molecular Pharmacology and Physiology, University of South Florida, Morsani College of Medicine, Tampa, Florida, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kara","family":"Snyder","sequence":"additional","affiliation":[{"name":"USF Health Heart Institute, University of South Florida, Morsani College of Medicine, Tampa, Florida, USA."},{"name":"Department of Molecular Pharmacology and Physiology, University of South Florida, Morsani College of Medicine, Tampa, Florida, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Keri","family":"Janesko-Feldman","sequence":"additional","affiliation":[{"name":"Safar Center for Resuscitation Research, UPMC Children's Hospital of Pittsburgh, Rangos Research Center, Pittsburgh, Pennsylvania, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kiersten","family":"Gorse","sequence":"additional","affiliation":[{"name":"USF Health Heart Institute, University of South Florida, Morsani College of Medicine, Tampa, Florida, USA."},{"name":"Department of Molecular Pharmacology and Physiology, University of South Florida, Morsani College of Medicine, Tampa, Florida, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vincent A.","family":"Vagni","sequence":"additional","affiliation":[{"name":"Safar Center for Resuscitation Research, UPMC Children's Hospital of Pittsburgh, Rangos Research Center, Pittsburgh, Pennsylvania, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Patrick M.","family":"Kochanek","sequence":"additional","affiliation":[{"name":"Safar Center for Resuscitation Research, UPMC Children's Hospital of Pittsburgh, Rangos Research Center, Pittsburgh, Pennsylvania, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Travis C.","family":"Jackson","sequence":"additional","affiliation":[{"name":"USF Health Heart Institute, University of South Florida, Morsani College of Medicine, Tampa, Florida, USA."},{"name":"Department of Molecular Pharmacology and Physiology, University of South Florida, Morsani College of Medicine, Tampa, Florida, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2022,3,23]]},"reference":[{"key":"e_1_3_6_2_2","doi-asserted-by":"publisher","DOI":"10.3389\/fnmol.2020.00126"},{"key":"e_1_3_6_3_2","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-05-1667"},{"issue":"15","key":"e_1_3_6_4_2","first-page":"708","article-title":"Up-regulation of the proapoptotic caspase 2 splicing isoform by a candidate tumor suppressor, RBM5","volume":"105","author":"Fushimi K.","year":"2008","unstructured":"Fushimi K., , Ray P., , Kar A., , Wang L., , Sutherland L.C., , and Wu J.Y. 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