{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T06:30:49Z","timestamp":1787121049579,"version":"build-2736575974"},"reference-count":37,"publisher":"SAGE Publications","issue":"23-24","license":[{"start":{"date-parts":[[2025,5,28]],"date-time":"2025-05-28T00:00:00Z","timestamp":1748390400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Journal of Neurotrauma"],"published-print":{"date-parts":[[2025,12,1]]},"abstract":"<jats:p>Respiratory failure is one of the greatest causes of morbidity and mortality after cervical lesions, the most common type of spinal cord injury (SCI). Fortunately, several pre-clinical and clinical studies have shown spontaneous, but limited, respiratory recovery after injury. However, there are still many unanswered questions about what is driving this recovery, so there is a growing need to further elucidate the neuroplastic potential of the phrenic network. Here, we investigated the structural plasticity of the right and left phrenic networks by analyzing perineuronal net (PNN) changes after a C2 hemisection (C2Hx) in mice. For this purpose, the right and left phrenic systems were traced with a pseudorabies virus, a trans-synaptic retrograde tracer applied to the diaphragm muscle, labeling the entire phrenic motor network. We found most PNN-bearing neurons within the ventral horn in na\u00efve animals, specifically around phrenic motoneurons (PhMNs), but not phrenic spinal interneurons. Right, but not left, C2Hx resulted in a significant increase in PNNs and glutamatergic synapses around ipsilateral PhMNs, suggesting that the right C2Hx requires greater neuroplasticity to overcome respiratory dysfunction. The results from this study uncover profound anatomical and functional asymmetries in left- and right-sided phrenic networks, underlying the complex nature of the spinal respiratory system, and contribute to a more advanced understanding of how the phrenic network adapts to trauma. Overall, this work underscores the importance of studying neuroplasticity and how it holds the potential to help improve outcomes for individuals living with SCI.<\/jats:p>","DOI":"10.1089\/neu.2024.0597","type":"journal-article","created":{"date-parts":[[2025,5,28]],"date-time":"2025-05-28T18:06:12Z","timestamp":1748455572000},"page":"2204-2216","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":4,"title":["Perineuronal Net Changes Reveal a Distinct Right and Left Spinal Phrenic Circuit"],"prefix":"10.1177","volume":"42","author":[{"given":"Judith","family":"S\u00e1nchez-Ventura","sequence":"first","affiliation":[{"name":"Department Cell Biology, Physiology and Immunology, Institute of Neuroscience, Universitat Aut\u00f2noma de Barcelona, and Centro de Investigaci\u00f3n Biom\u00e9dica en Red sobre Enfermedades Neurodegenerativas (CIBERNED), Bellaterra, Spain."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kayla Anne","family":"Schardien","sequence":"additional","affiliation":[{"name":"Department of Neurobiology and Anatomy, College of Medicine, Drexel University, Philadelphia, Pennsylvania, USA."},{"name":"The Marion Murray Spinal Cord Research Center, College of Medicine, Drexel University, Philadelphia, Pennsylvania, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tara","family":"Fortino","sequence":"additional","affiliation":[{"name":"Department of Neurobiology and Anatomy, College of Medicine, Drexel University, Philadelphia, Pennsylvania, USA."},{"name":"The Marion Murray Spinal Cord Research Center, College of Medicine, Drexel University, Philadelphia, Pennsylvania, USA."},{"name":"International Collaboration on Repair Discoveries, University of British Columbia, Vancouver, Canada."},{"name":"Centre for Chronic Disease Prevention and Management, University of British Columbia, Kelowna, Canada."},{"name":"Faculty of Medicine, Department of Cellular and Physiological Sciences, University of British Columbia, Vancouver, Canada."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4995-8466","authenticated-orcid":false,"given":"Lana V.","family":"Zholudeva","sequence":"additional","affiliation":[{"name":"Gladstone Institute of Cardiovascular Disease, Gladstone Institutes, San Francisco, California, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1375-6483","authenticated-orcid":false,"given":"Michael A.","family":"Lane","sequence":"additional","affiliation":[{"name":"Department of Neurobiology and Anatomy, College of Medicine, Drexel University, Philadelphia, Pennsylvania, USA."},{"name":"The Marion Murray Spinal Cord Research Center, College of Medicine, Drexel University, Philadelphia, Pennsylvania, 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