{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T00:05:34Z","timestamp":1790899534894,"version":"4.1.0"},"reference-count":0,"publisher":"SAGE Publications","issue":"1","license":[{"start":{"date-parts":[[2005,3,1]],"date-time":"2005-03-01T00:00:00Z","timestamp":1109635200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["OMICS: A Journal of Integrative Biology"],"published-print":{"date-parts":[[2005,3]]},"abstract":"\n Eight nucleotide sequences containing a single rhodanese domain were found in the\n Acidithiobacillus ferrooxidans<\/jats:italic>\n ATCC 23270 genome:\n p11, p14, p14.3, p15, p16, p16.2, p21,<\/jats:italic>\n and\n p28<\/jats:italic>\n . Amino acids sequence comparisons allowed us to identify the potentially catalytic\nCys residues and other highly conserved rhodanese family features in all eight proteins. The\ngenomic contexts of some of the rhodanese-like genes and the determination of their expression\nat the mRNA level by using macroarrays suggested their implication in sulfur oxidation\nand metabolism, formation of Fe-S clusters or detoxification mechanisms. Several of\nthe putative rhodanese genes were successfully isolated, cloned and overexpressed in\n E. coli<\/jats:italic>\n and their thiosulfate:cyanide sulfurtransferase (TST) and 3-mercaptopyruvate\/cyanide sulfurtransferase\n(MST) activities were determined. Based on their sulfurtransferase activities\nand on structural comparisons of catalytic sites and electrostatic potentials between homology-\nmodeled\n A. ferrooxidans<\/jats:italic>\n rhodaneses and the reported crystal structures of\n E. coli<\/jats:italic>\n GlpE\n(TST) and SseA (MST) proteins, two of the rhodanese-like proteins (P15 and P16.2) could\nclearly be defined as TSTs, and P14 and P16 could possibly correspond to MSTs. Nevertheless,\nseveral of the eight\n A. ferrooxidans<\/jats:italic>\n rhodanese-like proteins may have some different\nfunctional activities yet to be discovered.\n <\/jats:p>","DOI":"10.1089\/omi.2005.9.13","type":"journal-article","created":{"date-parts":[[2005,4,1]],"date-time":"2005-04-01T09:44:09Z","timestamp":1112348649000},"page":"13-29","source":"Crossref","is-referenced-by-count":40,"title":["Identification of Putative Sulfurtransferase Genes in the Extremophilic\n Acidithiobacillus ferrooxidans<\/i>\n ATCC 23270 Genome: Structural and Functional Characterization of the Proteins"],"prefix":"10.1177","volume":"9","author":[{"given":"Mauricio","family":"Acosta","sequence":"first","affiliation":[{"name":"Laboratory of Molecular Microbiology and Biotechnology, and Millennium Institute for Advanced Studies in Cell Biology and Biotechnology (CBB), Department of Biology, Faculty of Sciences, University of Chile, Santiago, Chile."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Simon","family":"Beard","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Microbiology and Biotechnology, and Millennium Institute for Advanced Studies in Cell Biology and Biotechnology (CBB), Department of Biology, Faculty of Sciences, University of Chile, Santiago, Chile."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jose","family":"Ponce","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Microbiology and Biotechnology, and Millennium Institute for Advanced Studies in Cell Biology and Biotechnology (CBB), Department of Biology, Faculty of Sciences, University of Chile, Santiago, Chile."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mario","family":"Vera","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Microbiology and Biotechnology, and Millennium Institute for Advanced Studies in Cell Biology and Biotechnology (CBB), Department of Biology, Faculty of Sciences, University of Chile, Santiago, Chile."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juan C.","family":"Mobarec","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Microbiology and Biotechnology, and Millennium Institute for Advanced Studies in Cell Biology and Biotechnology (CBB), Department of Biology, Faculty of Sciences, University of Chile, Santiago, Chile."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carlos A.","family":"Jerez","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Microbiology and Biotechnology, and Millennium Institute for Advanced Studies in Cell Biology and Biotechnology (CBB), Department of Biology, Faculty of Sciences, University of Chile, Santiago, Chile."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2005,3]]},"container-title":["OMICS: A Journal of Integrative Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1089\/omi.2005.9.13","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1089\/omi.2005.9.13","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T22:53:03Z","timestamp":1777675983000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/full\/10.1089\/omi.2005.9.13"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2005,3]]},"references-count":0,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2005,3]]}},"alternative-id":["10.1089\/omi.2005.9.13"],"URL":"https:\/\/doi.org\/10.1089\/omi.2005.9.13","relation":{},"ISSN":["1536-2310","1557-8100"],"issn-type":[{"value":"1536-2310","type":"print"},{"value":"1557-8100","type":"electronic"}],"subject":[],"published":{"date-parts":[[2005,3]]}}}