{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T15:09:31Z","timestamp":1791212971612,"version":"4.1.0"},"reference-count":34,"publisher":"SAGE Publications","issue":"2","license":[{"start":{"date-parts":[[2013,2,1]],"date-time":"2013-02-01T00:00:00Z","timestamp":1359676800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["OMICS: A Journal of Integrative Biology"],"published-print":{"date-parts":[[2013,2]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    We have shown earlier that fluconazole (FLC) stress induces global changes in the lipidome of\n                    <jats:italic toggle=\"yes\">Candida albicans<\/jats:italic>\n                    in clinically adapted isolates. However, several laboratories have developed adapted\n                    <jats:italic toggle=\"yes\">in vitro<\/jats:italic>\n                    FLC resistant strains of\n                    <jats:italic toggle=\"yes\">C. albicans<\/jats:italic>\n                    to study azole resistance mechanisms. This study aimed to identify the lipid changes associated with FLC resistance in these\n                    <jats:italic toggle=\"yes\">in vitro<\/jats:italic>\n                    adapted isolates. Using comparative lipidomics and principal component and discriminant analyses, we observed gradual changes in several lipid classes and molecular species upon FLC exposure of\n                    <jats:italic toggle=\"yes\">in vitro<\/jats:italic>\n                    resistant\n                    <jats:italic toggle=\"yes\">C. albicans<\/jats:italic>\n                    strains. Although the lipid imprint of FLC\n                    <jats:italic toggle=\"yes\">in vitro<\/jats:italic>\n                    resistant isolates was very distinct from that of clinical isolates of\n                    <jats:italic toggle=\"yes\">C. albicans<\/jats:italic>\n                    , the overall changes in lipid class compositions were similar in both cases. For example, an increased sterol content and depleted sphingolipid levels were the salient features of FLC resistance in both conditions. Taken together, it appears that the overall cellular lipid homeostasis is a critical factor in the observed FLC resistance and in handling FLC stress in both clinical and laboratory situations. The new observations reported herein have implications for more efficacious antifungal drug development as well as understanding host\u2013infectious agent interactions in postgenomics microbiology practice.\n                  <\/jats:p>","DOI":"10.1089\/omi.2012.0075","type":"journal-article","created":{"date-parts":[[2013,2,1]],"date-time":"2013-02-01T13:32:49Z","timestamp":1359725569000},"page":"84-93","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":28,"title":["Lipidomics and\n                    <i>in Vitro<\/i>\n                    Azole Resistance in\n                    <i>Candida albicans<\/i>"],"prefix":"10.1177","volume":"17","author":[{"given":"Ashutosh","family":"Singh","sequence":"first","affiliation":[{"name":"Jawaharlal Nehru University","place":["India"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kaushal Kumar","family":"Mahto","sequence":"additional","affiliation":[{"name":"Jawaharlal Nehru University","place":["India"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rajendra","family":"Prasad","sequence":"additional","affiliation":[{"name":"Jawaharlal Nehru University","place":["India"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2013,2]]},"reference":[{"key":"e_1_3_3_2_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.40.12.2835"},{"key":"e_1_3_3_3_1","doi-asserted-by":"publisher","DOI":"10.1139\/y59-099"},{"key":"e_1_3_3_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2009.07018.x"},{"key":"e_1_3_3_5_1","doi-asserted-by":"publisher","DOI":"10.1097\/00001432-200212000-00003"},{"key":"e_1_3_3_6_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.182.6.1515-1522.2000"},{"key":"e_1_3_3_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2010.07503.x"},{"key":"e_1_3_3_8_1","doi-asserted-by":"publisher","DOI":"10.1194\/jlr.D001768"},{"key":"e_1_3_3_9_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.91.22.10635"},{"key":"e_1_3_3_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tcb.2006.08.009"},{"key":"e_1_3_3_11_1","doi-asserted-by":"publisher","DOI":"10.1042\/BJ20091882"},{"key":"e_1_3_3_12_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.46.4.1046-1052.2002"},{"key":"e_1_3_3_13_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.43.7.1621"},{"key":"e_1_3_3_14_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12281-009-0021-y"},{"key":"e_1_3_3_15_1","first-page":"123","article-title":"The extraction and analysis of sterols from yeast","volume":"53","author":"Michael AQ","year":"2008","unstructured":"Michael AQ, , Kelly SL. 2008. The extraction and analysis of sterols from yeast. Methods Mol Biol, 53:123\u2013131.","journal-title":"Methods Mol Biol"},{"key":"e_1_3_3_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0925-4439(02)00087-X"},{"key":"e_1_3_3_17_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.46.12.3695-3705.2002"},{"key":"e_1_3_3_18_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.48.5.1778-1787.2004"},{"key":"e_1_3_3_19_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00861-07"},{"key":"e_1_3_3_20_1","doi-asserted-by":"publisher","DOI":"10.1042\/BST0331219"},{"key":"e_1_3_3_21_1","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00029-06"},{"key":"e_1_3_3_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0074-7696(04)42005-1"},{"key":"e_1_3_3_23_1","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dki218"},{"key":"e_1_3_3_24_1","doi-asserted-by":"publisher","DOI":"10.1038\/nbt0308-303"},{"key":"e_1_3_3_25_1","first-page":"197","volume-title":"Molecular Principles of Fungal Pathogenesis","author":"Sanglard D","year":"2005","unstructured":"Sanglard D, , White TC. 2005. Chapter 14. Molecular Principles of Antifungal Drug Resistance. Molecular Principles of Fungal Pathogenesis. Heitman J, , Filler SG, , Edwards JE, , Mitchell AP. 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