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Protein phosphorylation has long been relevant to sperm physiology, from acquisition of motility in the epididymis to capacitation in the female reproductive tract. While the precise kinases involved in the regulation of sperm phosphorylation have been studied for decades, the PPs have only recently received research interest. Tyrosine phosphorylation was first implicated in the regulation of several sperm-related functions, from capacitation to oocyte binding. Only afterwards, in 1996, the inhibition of the serine\/threonine-PP phosphoprotein phosphatase 1 (PPP1) by okadaic acid and calyculin-A was shown to initiate motility in caput epididymal sperm. Today, the current mechanisms of sperm motility acquisition based on PPP1 and its regulators are still far from being fully understood. PPP1CC2, specifically expressed in mammalian sperm, has been considered to be the only sperm-specific serine\/threonine-PP, while other PPP1 isoforms were thought to be absent from sperm. This article examines the \u201cOmics\u201d of human sperm, and reports, for the first time, the identification of three new serine\/threonine-protein PPs, PPP1CB, PPP4C, and PPP6C, in human sperm, together with two tyrosine-PPs, MKP1 and PTP1C. We specifically localized in sperm PPP1CB and PPP1CC2 from the PPP1 subfamily, and PPP2CA, PPP4C, and PPP6C from the PPP2 subfamily of the serine\/threonine-PPs. A semi-quantitative analysis was performed to determine the various PPs' differential expression in sperm head and tail. These findings contribute to a comprehensive understanding of human sperm PPs, and warrant further research for their clinical and therapeutic significance.<\/jats:p>","DOI":"10.1089\/omi.2012.0119","type":"journal-article","created":{"date-parts":[[2013,7,29]],"date-time":"2013-07-29T09:09:31Z","timestamp":1375088971000},"page":"460-472","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":21,"title":["\u201cOMICS\u201d of Human Sperm: Profiling Protein Phosphatases"],"prefix":"10.1177","volume":"17","author":[{"given":"Margarida","family":"Fardilha","sequence":"first","affiliation":[{"name":"Universidade de Aveiro","place":["Portugal"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M\u00f3nica","family":"Ferreira","sequence":"additional","affiliation":[{"name":"Universidade de Aveiro","place":["Portugal"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Steven","family":"Pelech","sequence":"additional","affiliation":[{"name":"Kinexus Bioinformatics Corporation","place":["Canada"]},{"name":"University of British Columbia","place":["Canada"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sandra","family":"Vieira","sequence":"additional","affiliation":[{"name":"Universidade de Aveiro","place":["Portugal"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sandra","family":"Rebelo","sequence":"additional","affiliation":[{"name":"Universidade de Aveiro","place":["Portugal"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lu\u00eds","family":"Korrodi-Gregorio","sequence":"additional","affiliation":[{"name":"Universidade de Aveiro","place":["Portugal"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M\u00e1rio","family":"Sousa","sequence":"additional","affiliation":[{"name":"Instituto de Ci\u00eancias Biom\u00e9dicas Abel Salazar","place":["Portugal"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alberto","family":"Barros","sequence":"additional","affiliation":[{"name":"Universidade do Porto","place":["Portugal"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vladimiro","family":"Silva","sequence":"additional","affiliation":[{"name":"Centro de Estudos de Fertilidade de Coimbra","place":["Portugal"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Odete A. 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