{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T12:40:54Z","timestamp":1790253654575,"version":"4.1.0"},"reference-count":57,"publisher":"SAGE Publications","issue":"10","license":[{"start":{"date-parts":[[2013,10,1]],"date-time":"2013-10-01T00:00:00Z","timestamp":1380585600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["OMICS: A Journal of Integrative Biology"],"published-print":{"date-parts":[[2013,10]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Leptospirosis is one of the most important zoonoses.\n                    <jats:italic toggle=\"yes\">Leptospira interrogans<\/jats:italic>\n                    serovar Lai is a pathogenic spirochete that is responsible for leptospirosis. Extracellular proteins play an important role in the pathogenicity of this bacterium. In this study,\n                    <jats:italic toggle=\"yes\">L. interrogans<\/jats:italic>\n                    serovar Lai was grown in protein-free medium; the supernatant was collected and subsequently analyzed as the extracellular proteome. A total of 66 proteins with more than two unique peptides were detected by MS\/MS, and 33 of these were predicted to be extracellular proteins by a combination of bioinformatics analyses, including Psortb, cello, SoSuiGramN and SignalP. Comparisons of the transcriptional levels of these 33 genes between\n                    <jats:italic toggle=\"yes\">in vivo<\/jats:italic>\n                    and\n                    <jats:italic toggle=\"yes\">in vitro<\/jats:italic>\n                    conditions revealed that 15 genes were upregulated and two genes were downregulated\n                    <jats:italic toggle=\"yes\">in vivo<\/jats:italic>\n                    compared to\n                    <jats:italic toggle=\"yes\">in vitro<\/jats:italic>\n                    . A BLAST search for the components of secretion system at the genomic and proteomic levels revealed the presence of the complete type I secretion system and type II secretion system in this strain. Moreover, this strain also exhibits complete Sec translocase and Tat translocase systems. The extracellular proteome analysis of\n                    <jats:italic toggle=\"yes\">L. interrogans<\/jats:italic>\n                    will supplement the previously generated whole proteome data and provide more information for studying the functions of specific proteins in the infection process and for selecting candidate molecules for vaccines or diagnostic tools for leptospirosis.\n                  <\/jats:p>","DOI":"10.1089\/omi.2013.0043","type":"journal-article","created":{"date-parts":[[2013,7,29]],"date-time":"2013-07-29T09:09:31Z","timestamp":1375088971000},"page":"527-535","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":27,"title":["Extracellular Proteome Analysis of\n                    <i>Leptospira interrogans<\/i>\n                    serovar Lai"],"prefix":"10.1177","volume":"17","author":[{"given":"Lingbing","family":"Zeng","sequence":"first","affiliation":[{"name":"Shanghai Jiao Tong University School of Medicine","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yunyi","family":"Zhang","sequence":"additional","affiliation":[{"name":"Zhejiang Provincial Center for Disease Control and Prevention","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yongzhang","family":"Zhu","sequence":"additional","affiliation":[{"name":"Shanghai Jiao Tong University School of Medicine","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haidi","family":"Yin","sequence":"additional","affiliation":[{"name":"The University of Michigan Medical Center"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xuran","family":"Zhuang","sequence":"additional","affiliation":[{"name":"Shanghai Jiao Tong University School of Medicine","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weinan","family":"Zhu","sequence":"additional","affiliation":[{"name":"Shanghai Jiao Tong University School of Medicine","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaokui","family":"Guo","sequence":"additional","affiliation":[{"name":"Shanghai Jiao Tong University School of Medicine","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinhong","family":"Qin","sequence":"additional","affiliation":[{"name":"Shanghai Jiao Tong University School of Medicine","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2013,10]]},"reference":[{"key":"e_1_3_3_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2011.02.055"},{"key":"e_1_3_3_3_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2180-5-58"},{"key":"e_1_3_3_4_1","first-page":"783795","article-title":"Improved prediction of signal peptides: SignalP 3.0","volume":"340","author":"Bendtsen JD","year":"2004","unstructured":"Bendtsen JD, , Nielsen H, , Von Heijne G, , Brunak S. 2004. 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